The Apoptotic, Cytotoxic, and Antiangiogenic Impact of <i>Linum usitatissimum</i> Seed Essential Oil Nanoemulsions on the Human Ovarian Cancer Cell Line A2780

Keykhasalar, Roghaye; Tabrizi, Masoud Homayouni; Ardalan, Pouran; Khatamian, Niloufar

doi:10.1080/01635581.2020.1824001

Cited by 19 publications

(7 citation statements)

References 40 publications

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“…Flavonoids, in particular, have substantial antiangiogenic effects through regulating the expression of VEGF, matrix metalloproteinases (MMPs), and EGFR, as well as inhibiting the NFB, PI3-K/Akt, and ERK1/2 signaling pathways (Rizvi, 2021).In a study published in 2020, Keykhasalar and coworkers found that flaxseed oil nanoemulsion reduced the length and number of vessels in a CAM assay, proving that flaxseed oil nanoemulsion has antiangiogenic activity. This study supports the antiangiogenic effect produced by flaxseed oil in the in vivo CAM assay in the current study (Keykhasalar et al, 2020).…”

Section: Discussionsupporting

confidence: 90%

The Antiangiogenic Activity of Flaxseed Oil Alone and Combination with Mefenamic Acid in Vivo and in Vitro Assay

Al-Zubaidy

Sahib

2022

Asian Pac J Cancer Prev

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Background: Antiangiogenic agents are commonly used for the management of many types of cancers. Flaxseed oil is a wealthy source of omega-3,omega-6, and lignans, and possesses anticancerous and antioxidant activities. Objective: To investigate the antiangiogenic activity of flaxseed oil alone and in combination with mefenamic acid in a dose-response study. Methods: Oil was extracted from flaxseeds. The ex vivo rat aorta ring assay was used to screen the flaxseed oil alone and in combination with mefenamic acid for possible antiangiogenic activity. Also, the assay was used to determine the dose-response effect.An in vivo chick chorioallantoic membrane (CAM) assay was used to quantify the zone of inhibition of blood vessel by flaxseed oil. Results: The 200,100,50,25,12.5, and 6.25µg/ ml of the flaxseed oil inhibited blood vessel growth with values of 91±0.42, 84±2.06,36±2.71, 21±2.15,23±1.56, and 5±0.93%, respectively, withan IC 50 value of 45.695 µg/ml. These concentrations showed a dose-dependent inhibition of angiogenesis. At 200, 100, and 50 µg/ml of flaxseed oil in combination with 50 µg/ml of mefenamic acid inhibited blood vessel growth with inhibition percentages of 81.48±0.82,79.63±0.75, and 77.78±1.26, respectively, with an IC 50 of 2.27 µg/ml. Also, these concentrations indicated a dose-dependent inhibition of angiogenesis. Flaxseed oil produced a significant inhibition zone of blood vessels in the CAM assay. The LD 50 for flaxseed oil was 5.656g/kg. Conclusion: Flaxseed oil alone and in combination with mefenamic acid exhibited antiangiogenic activity both in ex vivo and in vivo assays. At doses of 2.5, 1.25, 0.625, and 0.312 g/kg of flaxseed oil intraperitoneally injected into mice, no symptoms of toxicity or death of mice due to acute toxicity were observed. However, doses of 5 and 10 g/kg of oil resulted in the death of the majority of mice.

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Section: Discussionsupporting

confidence: 90%

The Antiangiogenic Activity of Flaxseed Oil Alone and Combination with Mefenamic Acid in Vivo and in Vitro Assay

Al-Zubaidy

Sahib

2022

Asian Pac J Cancer Prev

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“…35 The properties of NEs such as greater surface area, modifiable surface charge, higher circulation half-life, precise targeting, and formulation imaging capability that are necessary to create an effective therapeutic impact have drawn increased attention in the field of cancer therapy research. 45 NEs, because of their versatile characteristic features, have been a preferred choice of researchers in treating several types of cancer including colorectal, 46 breast, 47 ovarian, 48 lung, 49 brain, 50 leukemia, 51 prostate, 52 and melanoma. 53 NEs ranging in size from 20 to 100 nm can get encapsulated and accumulate in tumor tissues (Figure 2) because they are both large enough to evade rapid renal clearance and small enough to flow through blood arteries.…”

Section: Nanoemulsion: a Potential Carrier For Delivering Polyphenols...mentioning

confidence: 99%

“…NEs, because of their versatile characteristic features, have been a preferred choice of researchers in treating several types of cancer including colorectal, breast, ovarian, lung, brain, leukemia, prostate, and melanoma . NEs ranging in size from 20 to 100 nm can get encapsulated and accumulate in tumor tissues (Figure ) because they are both large enough to evade rapid renal clearance and small enough to flow through blood arteries .…”

Section: Nanoemulsion: a Potential Carrier For Delivering Polyphenols...mentioning

confidence: 99%

Therapeutic Implications of Dietary Polyphenols-Loaded Nanoemulsions in Cancer Therapy

Tomar,

Das,

Balaga

et al. 2024

ACS Appl. Bio Mater.

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Cancer is one of the major causes of death worldwide, even the second foremost cause related to non-communicable diseases. Cancer cells typically possess several cellular and biological processes including, persistence, propagation, differentiation, cellular death, and expression of cellular-type specific functions. The molecular picture of carcinogenesis and progression is unwinding, and it appears to be a tangled combination of processes occurring within and between cancer cells and their surrounding tissue matrix. Polyphenols are plant secondary metabolites abundant in fruits, vegetables, cereals, and other natural plant sources. Natural polyphenols have implicated potential anticancer activity by various mechanisms involved in their antitumor action, including modulation of signaling pathways majorly related to cellular proliferation, differentiation, relocation, angiogenesis, metastatic processes, and cell death. The applications of polyphenols have been limited due to the hydrophobic nature and lower oral bioavailability that could be possibly overcome through encapsulating them into nanocarrier-mediated delivery systems, leading to improved anticancer activity. Nanoemulsions (NEs) possess diverse feasible properties, including greater surface area, modifiable surficial charge, higher half-life, site-specific targeting, and formulation imaging capability necessary to create a practical therapeutic impact, and have drawn increased attention in cancer therapy research. This review has summarized and discussed the basic concepts, classification, delivery approaches, and anticancer mechanism of various polyphenols and polyphenols-encapsulated nanoemulsions with improved cancer therapy.

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“…Linseed (Linum usitatissimum Linn), a plant from the Linaceae family, is an example of functional food with rising demand by population, in its various forms (seed, bran, oil), because of its important nutritional components, such as essential fatty acids, fiber and proteins (Rosling, 1993;Attia et al, 2022abc;Shim et al, 2022;Sirotkin, 2023). Linseed protein have been reported to possess anti-diabetic, anti-inflammatory, antihypertensive and antioxidative properties (Dzuvor et al, 2018;Keykhasalar et al, 2021;Attia et al, 2022abc;Mueed et al, 2022ab;Sirotkin, 2023). What is not known by population, however, is that, in addition to these healthy nutritional compounds, linseed contain the cyanogenic glycosides linamarin, linustatin, neolinustatin and lotaustralin (Shim et al, 2014;Abraham, Buhrke, Lampen, 2016;Mueed et al, 2022ab), which can be harmful to human health.…”

Section: Introductionmentioning

confidence: 99%

Determination of cyanide in whole seeds and brans of linseed (Linum usitatissimum Linn) by molecular spectrophotometry

Pereira,

Souza,

Medeiros

et al. 2023

Braz. J. Pharm. Sci.

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The addition of linseed (Linum usitatissimum Linn) in the diet, as a functional food, has increased over the years. However, it possesses cyanogenic glycosides. This study aimed to quantify and compare cyanide concentration in whole seed and bran of brown and golden types to establish a safe limit of intake. Three commercial labels, from brown and golden whole seed types (Ab, Ag, Bb, Bg, Cb and Cg), and six commercial labels of brown and golden bran (1b, 2g, 3g, 4b, 5g, and 6b), were selected, totalizing twelve samples. Total cyanide concentration was quantified by a colorimetric method employing alkaline picrate, after acid hydrolysis. The whole seed cyanide values were between 348.4 and 473.20 µg/g and the bran cyanide values were between 459.53 and 639.35 μg/g. The analyzed bran presented increased cyanide concentrations than the whole seeds with no differences between brown and golden types. Food able to produce cyanide less than 90 µg/kg body weight, daily, is considered secure for consumption. Considering this limit and analyzed samples, it is safe to eat approximately two tablespoons of seeds or one tablespoon of bran. These results point out the importance of cyanide amount daily intake information to be in linseed packaging, to ensure secure consumption.

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The Apoptotic, Cytotoxic, and Antiangiogenic Impact of Linum usitatissimum Seed Essential Oil Nanoemulsions on the Human Ovarian Cancer Cell Line A2780

Cited by 19 publications

References 40 publications

The Antiangiogenic Activity of Flaxseed Oil Alone and Combination with Mefenamic Acid in Vivo and in Vitro Assay

The Antiangiogenic Activity of Flaxseed Oil Alone and Combination with Mefenamic Acid in Vivo and in Vitro Assay

Therapeutic Implications of Dietary Polyphenols-Loaded Nanoemulsions in Cancer Therapy

Determination of cyanide in whole seeds and brans of linseed (Linum usitatissimum Linn) by molecular spectrophotometry

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