Pinus morrisonicola needles essential oil nanoemulsions as a novel strong antioxidant and anticancer agent

Khatamian, Niloufar; Soltani, Mohammad Reza; Shadan, Behnaz; Neamati, Ali; Tabrizi, Masoud Homayouni; Hormozi, Bahareh

doi:10.1080/24701556.2021.1892760

Cited by 17 publications

(4 citation statements)

References 59 publications

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“…CS–GA (0.500 g) was weighed and dissolved in 100 mL of deionized water to form a solution, and the supernatant was centrifuged at 2500 r/min after magnetic stirring for 2 h [ 41 ]. DHA algal oil (0.500 g) was added to the obtained supernatant, and a crude emulsion was first made using high-speed shearing; then, the crude emulsion was further processed using a high-pressure homogenizer a certain number of times to obtain a nanoemulsion.…”

Section: Methodsmentioning

confidence: 99%

Synthesis and Characterization of Emulsifiers Based on the Maillard Reaction and Its Application in Stabilized DHA Algal Oil Nanoemulsions

Yan,

Hu,

Gao

et al. 2024

Foods

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The aim of this study was to optimize the formation of sodium caseinate (CS) and gum arabic (GA) complexes through the Maillard reaction and to evaluate their effectiveness in improving the emulsification properties and stability of docosahexaenoic acid (DHA) nanoemulsions. First, the best target polysaccharides were selected, and the best modification conditions were determined using orthogonal experiments. Secondly, the response surface experiments were used to optimize the preparation process of the emulsion. The stability, in vitro digestion characteristics, and rheological characteristics of the emulsion prepared by means of CS–GA were compared with the emulsion prepared using a whey protein isolate (WPI). After the orthogonal test, the optimal modification conditions were determined to be a reaction time of 96 h, a CS–GA mass ratio of 1:2, a reaction temperature of 60 °C, and a degree of grafting of 44.91%. Changes in the infrared (IR), Raman, ultraviolet (UV), and endogenous fluorescence spectra also indicated that the complex structure was modified. The response surface test identified the optimal preparation process as follows: an emulsifier concentration of 5 g/L, an oil-phase concentration of 5 g/L, and a homogenization frequency of five, and the emulsion showed good stability. Therefore, the use of a nanoemulsion as a nanoscale DHA algal oil delivery system is very promising for extending the shelf life and improving the stability of food.

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Section: Methodsmentioning

confidence: 99%

Synthesis and Characterization of Emulsifiers Based on the Maillard Reaction and Its Application in Stabilized DHA Algal Oil Nanoemulsions

Yan,

Hu,

Gao

et al. 2024

Foods

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“…A leaf EO of P. eldarica was more active against HeLa and MCF-7 cells than two different hydro-alcoholic extracts from the same species [ 273 ]. A needle EO of Pinus morrisonicola Hayata showed a somewhat selective inhibitory effect on several malignant cell lines (A549—human lung cancer, HepG2—hepatoma, MCF-7—breast cancer, PC3—prostate cancer, and HT-29—colon cancer) compared with normal human fibroblasts (HFF cells) through apoptosis, as indicated by the upregulation of several pro-apoptotic genes [ 274 ]. A fresh needle EO from P. wallichiana (with β-pinene—46.8% and α-pinene—25.2% as the key components) exerted antiproliferative effects on several cancer cell lines: THP-1 (leukemia), A-549 (lung cancer), HEP-2 (liver cancer), IGR-OV-1 (ovarian cancer), and PC-3 (prostate cancer); IC 50 values for all lines varied between 5.6 and 9.9 μg/mL [ 72 ].…”

Section: Pharmacological Interest Of Pinaceae Eosmentioning

confidence: 99%

Antioxidant Activity of Essential Oils from Pinaceae Species

Ancuceanu,

Anghel,

Hovaneț

et al. 2024

Antioxidants

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With a widespread distribution throughout the Northern Hemisphere and 11 genera, Pinaceae is the largest family of Gymnosperms in the world. Essential oils are an important chemotaxonomic marker for the species of this family, although the degree of chemical and biological investigation has not been the same for all genera. Essential oils from Abies and Cedrus (from the abietoid clade) or Pinus and Picea (from the pinoid clade) have been more extensively investigated with respect to their chemical composition and biological or pharmacological properties, including their antioxidant effects. Instead, essential oils from the other genera of the family have been less explored in this respect or even have not been investigated at all. This is a narrative review looking into the knowledge acquired up to date, the variability and limitations of the current methods used to estimate antioxidant effects, and multiple comparisons between EOs obtained from different genera, species, and plant parts, as well as potential applications and future directions of research and utilization of essential oils derived from Pinaceae species.

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“…The cells' staining was carried out by adding 1 μL uorescent staining solution containing AO (100 μg/mL) and PI (100 μg/mL) to each cell suspension droplets (25 μL) and then covered with a coverslip. The apoptotic cell morphology was studied utilizing a uorescent microscope (OLYMPUS, Japan) (Khatamian et al 2021).…”

Section: Gene Expression Measurementsmentioning

confidence: 99%

In Vitro and In Vivo Anticancer Activity of Ferula Gummosa Essential Oil Nanoemulsions (FGEO-NE) for the Colon Cancer Treatment

et al. 2022

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This survey was performed to aim of synthesize Nano emulsion from Ferula gummosa essential oil (FEGO-NE) and to evaluate its anti-tumor effect.First, Ferula gummosa essential oil was analyzed by GC-MS method, then the Nano emulsion was synthesized as O/W and after characterization by DLS, Zeta potential, AFM, FESEM and TEM methods, its toxicity was evaluated by MTT method. Then its pro-apoptotic effects were evaluated by qPCR (Caspase3, 9, Bax and Bcl-2) method and AO/PI staining. The cancer induction model was used to evaluate the antitumor effects in Balb/C mice. The anti-angiogenic and antioxidant effects were evaluated by qPCR (VEGF, CAT and SOD) method.The results of physicochemical studies showed the formation of nanoparticles with dimensions of 24.6 nm, dispersion index of 0.41 and zeta potential of -28.5 mV with a spherical morphology. The Nano emulsion synthesized at a concentration of 2.9 μg/ml inhibited about 50% of ht-29 cells, while up to a concentration of 4 μg/ml showed no inhibitory effect on normal cells. Increase of caspase 3, 9 and Bax and decrease of BCL-2 gene expression along with increase of apoptotic cells in AP / PI staining con rmed induction of apoptosis by FEGO-NE. The FEGO-NE showed an inhibitory effect on angiogenesis and an additive effect on the expression of antioxidant genes. In addition, the reduction of tumor volume (69.72% in 14 days) in samples treated with FEGO-NE was con rmed. The results of this study showed that FEGO-NE by various mechanisms are able to inhibit cancer cells and have a reducing effect on induced tumors in the in vivo model. These results suggest FEGO-NE as a suitable candidate for cancer therapy studies.

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Pinus morrisonicola needles essential oil nanoemulsions as a novel strong antioxidant and anticancer agent

Cited by 17 publications

References 59 publications

Synthesis and Characterization of Emulsifiers Based on the Maillard Reaction and Its Application in Stabilized DHA Algal Oil Nanoemulsions

Synthesis and Characterization of Emulsifiers Based on the Maillard Reaction and Its Application in Stabilized DHA Algal Oil Nanoemulsions

Antioxidant Activity of Essential Oils from Pinaceae Species

In Vitro and In Vivo Anticancer Activity of Ferula Gummosa Essential Oil Nanoemulsions (FGEO-NE) for the Colon Cancer Treatment

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