ABSTRACT:A simple, precise and sensitive reverse-phase high performance liquid chromatographic (RP-HPLC) method has been developed for the quantitation of Escitalopram oxalate in pharmaceutical formulations. Chromatographic separation was achieved on a 250 × 4.6 mm, 5µ, C 18 column. The flow rate was 1ml/min and eluent was monitored by absorbance at 240 nm using a mixture of Acetonitrile and Buffer (pH 4.0) in the ratio of 25:75 (v/v). The retention times of Escitalopram oxalate was found to be 3.2 min. Calibration plots were linear in the concentration range of 2.5-80 µg mL -1 for Escitalopram oxalate. The total run time is 10 min. The proposed method was validated by testing its linearity, recovery, specificity, system suitability, precision (Interday and intraday), robustness and LOD/LOQ values and it was successfully employed for the determination of Escitalopram oxalate in pharmaceutical tablet formulations.
This paper presents a RP-HPLC method for simultaneous estimation of Escitalopram and clonazepam in pharmaceutical formulations. The process was carried out on on a 250 × 4.6 mm, 5µ, C 18 column. The flow rate was 1ml/min and eluent was monitored by absorbance at 248 nm using a mixture of Methanol and Buffer (pH 4.0) in the ratio of 90:10 (v/v). The retention times of Escitalopram and Clonazepam was found to be 3.22 and 4.29 min respectively. Calibration plots were linear in the concentration range of 2.5-80 µg mL -1 and 0.125-4 µg mL -1 for Escitalopram and clonazepam respectively. The total run time is 10 min. The proposed method was validated by testing its linearity, recovery, specificity, system suitability, precision (Interday and intraday), robustness and LOD/LOQ values and it was successfully employed for the simultaneous estimation of Escitalopram and clonazepam in pharmaceutical tablet formulations.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.