Justicia gendarussa Burm f. (Acanthaceae) is known for its activity as a male contraceptive and anti-HIV properties. The present study was designed to evaluate extracts of J. gendarussa for cytotoxicity activity against MOLT-4 cells. The cytotoxic activity of the fractionated-extract and 70% ethanol extracts of J. gendarussa leaves on MOLT-4 cells were evaluated using a WST-1 assay. The treatment cells, control cells without treatment and control media were also tested in duplicate. The absorbance was measured at a wavelength of 450 nm using a microplate absorbance reader (Bio-Rad). The average absorbance measures formazan produced by viable cells that metabolize the WST-1 reagent. Then the data was analyzed with regression analysis Microsoft Excel 2007 program to determine the concentration with 50% cell viability (50% Cytotoxicity Concentration, CC50). The CC50 values of the fractionated-extract and 70% ethanol extract of J. gendarussa leaves were 94 μg/ml and 78 μg/ml, respectively. The cytotoxicity of fractionated-extract and 70% ethanol extract of J. gendarussa leaves were not significantly different (p > 0.05). It can be concluded that the fractionated-extract and 70% ethanol extract of J. gendarussa leaves are not toxic to MOLT-4 cells.
Justicia gendarussa Burm f. (Acanthaceae) is known for its activity as a male contraceptive and anti-HIV properties. The present study was designed to evaluate extracts of J. gendarussa for cytotoxicity activity against MOLT-4 cells. The cytotoxic activity of the fractionated-extract and 70% ethanol extracts of J. gendarussa leaves on MOLT-4 cells were evaluated using a WST-1 assay. The treatment cells, control cells without treatment and control media were also tested in duplicate. The absorbance was measured at a wavelength of 450 nm using a microplate absorbance reader (Bio-Rad). The average absorbance measures formazan produced by viable cells that metabolize the WST-1 reagent. Then the data was analyzed with regression analysis Microsoft Excel 2007 program to determine the concentration with 50% cell viability (50% Cytotoxicity Concentration, CC50
ABSTRACT Yacon (Smallanthus sonchifolius (Poepp.) H.Rob) leave has an effect on blood glucose level and lipid profiles in the alloxan-induced rat. This study aimed to determine the activity of ethanol extract of yacon leaves in increasing glycogen synthesis in the liver and muscles and decreasing blood glucose levels on hyperglycemic hamsters with a high-fat diet. Twenty-four of male Syrian hamsters divided into 6 groups consisting of normal control, negative control without treatment, positive control was given metformin (61.67 mg/kg), dose I group treatment that given extract at 180 mg/kg, dose II group treatment that given extract at 360 mg/kg, and dose III group treatment that given extract at 720 mg/kg. All groups were induced by alloxan monohydrate and high-fat diet, except for the normal control group. Blood glucose levels were measured using a clinical spectrophotometer. The glycogen deposits were added with 0,2% anthrone-sulfuric acid and measured using spectrophotometer UV-Vis. Data were analyzed using one-way ANOVA and continued with the Tukey test. The ethanol extract of yacon leaves at 360 mg/kg and 720 mg/kg can increase glycogen synthesis in liver and muscle compare to metformin (P>0,05), and also can decrease blood glucose levels on hamster by 53.32% and 57.81%, respectively which is comparable (P> 0.05) with metformin by 60.12%. Keywords: blood glucose level, liver, muscle, glycogen, Smallanthus sonchifolius ABSTRAK Daun yakon (Smallanthus sonchifolius (Poepp.) H.Rob) memiliki pengaruh terhadap kadar glukosa darah dan profil lipid pada tikus yang diinduksi aloksan. Penelitian ini bertujuan untuk mengetahui aktivitas ekstrak etanol daun yakon dalam meningkatkan sintesis glikogen di hati dan otot, serta menurunkan kadar glukosa darah pada hamster hiperglikemia dengan diet tinggi lemak. Penelitian ini menggunakan hamster Syrian jantan sebanyak 24 ekor yang dikelompokan menjadi 6 kelompok, yaitu kelompok kontrol normal, kontrol negatif tanpa perlakuan, kontrol positif yang diberi metformin (61,67 mg/kg), kelompok ekstrak dosis I (180 mg/kg), ekstrak dosis II (360 mg/kg), dan ekstrak dosis III (720 mg/kg). Seluruh kelompok diinduksi aloksan monohidrat dan pakan tinggi lemak kecuali kelompok kontrol normal. Serum darah direaksikan dengan glucose liquicolor dan kadar glukosa darah diukur menggunakan spektrofotometer klinikal. Endapan glikogen ditambahkan dengan antrone-asam sulfat 0,2% dan diukur menggunakan spketrofotometer UV-Vis. Data dianalisis menggunakan ANOVA satu arah dan dilanjutkan dengan uji Tukey. Ekstrak etanol daun yakon dengan dosis 360 mg/kg dan 720 mg/kg dapat meningkatkan sintesis glikogen di hati dan otot hamster yang sebanding dengan metformin (P>0,05), serta dapat menurunkan kadar glukosa darah sebesar 53,3275% dan 57,8125% yang sebanding (P>0,05) dengan metformin sebesar 60,12%. Kata Kunci: kadar glukosa darah, hati, otot, glikogen, Smallanthus sonchifolius
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