Cytotoxicity of Justicia Gendarussa Burm F. Leaf Extracts on Molt-4 Cell

Abstract

Justicia gendarussa Burm f. (Acanthaceae) is known for its activity as a male contraceptive and anti-HIV properties. The present study was designed to evaluate extracts of J. gendarussa for cytotoxicity activity against MOLT-4 cells. The cytotoxic activity of the fractionated-extract and 70% ethanol extracts of J. gendarussa leaves on MOLT-4 cells were evaluated using a WST-1 assay. The treatment cells, control cells without treatment and control media were also tested in duplicate. The absorbance was measured at a wavelength of 450 nm using a microplate absorbance reader (Bio-Rad). The average absorbance measures formazan produced by viable cells that metabolize the WST-1 reagent. Then the data was analyzed with regression analysis Microsoft Excel 2007 program to determine the concentration with 50% cell viability (50% Cytotoxicity Concentration, CC50

Prediksi Farmakokinetik, Toksisitas, dan Aktivitas Enzim Protease HIV-1 Inhibitor dari Daun J. gendarussa

“…Hasil uji secara in vitro menggunakan ekstrak etanol 70%, ekstrak etanol 70% terfraksinasi, dan ekstrak air daun J. gendarussa menunjukkan aktivitas menghambat enzim reverse transcriptase HIV (Woradulayapinij et al, 2005;Prajogo et al, 2014). Penelitian terbaru uji ekstrak daun J. gendarussa pada kultur sel MOLT-4 yang terinfeksi HIV, hasilnya menunjukkan bahwa daun J. gendarussa dapat menghambat replikasi HIV melalui penurunan jumlah p24 dan penghambatan pembentukan syncytia (Widiyanti et al, 2015).…”