Cytotoxicity of Justicia Gendarussa Burm F. Leaf Extracts on Molt-4 Cell

Abstract

Justicia gendarussa Burm f. (Acanthaceae) is known for its activity as a male contraceptive and anti-HIV properties. The present study was designed to evaluate extracts of J. gendarussa for cytotoxicity activity against MOLT-4 cells. The cytotoxic activity of the fractionated-extract and 70% ethanol extracts of J. gendarussa leaves on MOLT-4 cells were evaluated using a WST-1 assay. The treatment cells, control cells without treatment and control media were also tested in duplicate. The absorbance was measured at a wavelength of 450 nm using a microplate absorbance reader (Bio-Rad). The average absorbance measures formazan produced by viable cells that metabolize the WST-1 reagent. Then the data was analyzed with regression analysis Microsoft Excel 2007 program to determine the concentration with 50% cell viability (50% Cytotoxicity Concentration, CC50). The CC50 values of the fractionated-extract and 70% ethanol extract of J. gendarussa leaves were 94 μg/ml and 78 μg/ml, respectively. The cytotoxicity of fractionated-extract and 70% ethanol extract of J. gendarussa leaves were not significantly different (p > 0.05). It can be concluded that the fractionated-extract and 70% ethanol extract of J. gendarussa leaves are not toxic to MOLT-4 cells.

Chemical Constituents and Bioactivities of Several Indonesian Plants Typically Used in Jamu

“…The result showed an elimination half-life (t 1/2) of 243 in urinary excretion of 4.44±2.14 h, and the rate constant of elimination (Kel) was 0.18±0.07/h. 84) The 70% ethanol extract of these leaves is not toxic to MOLT-4 cells using a water-soluble tetrazolium-1 (WST-1) assay, with CC 50 values of 78 µg/mL. 85) The extract of this plant reduced cumulus oophorus dispersibility in vitro and testosterone concentration in mouse serum; therefore it may reduce mouse spermatozoa hyaluronidase.…”

“…84) The 70% ethanol extract of these leaves is not toxic to MOLT-4 cells using a water-soluble tetrazolium-1 (WST-1) assay, with CC 50 values of 78 µg/mL. 85) The extract of this plant reduced cumulus oophorus dispersibility in vitro and testosterone concentration in mouse serum; therefore it may reduce mouse spermatozoa hyaluronidase. A pre-clinical study of an alkaloid free 70% ethanol extract of leaf extract has confirmed its male contraceptive activity.…”

Overview of the Justicia Genus: Insights into Its Chemical Diversity and Biological Potential

“…It is worth mentioning that there is currently relevant interest among research groups in evaluating a possible analgesic, anti-inflammatory and antiulcerogenic activity. A diversity of special metabolites is found in Justicia , mainly alkaloids [ 46 , 47 , 48 , 49 , 50 , 51 ], steroids [ 52 , 53 , 54 ], tannins [ 32 , 41 , 46 , 51 ], terpenoids [ 47 , 48 , 51 , 54 , 55 , 56 , 57 , 58 ], lignans [ 59 , 60 , 61 , 62 , 63 , 64 ] and flavonoids [ 16 , 20 , 21 , 22 , 23 , 24 , 31 , 32 , 51 , 65 , 66 , 67 ].…”

The Effect of Different Extracts of Beetroots as Antioxidant and Anti-Anaemia On Phenylhydrazine-Induced Rats

“…The first part, namely releasing-alkaloid beetroot powder. The dried powder (820.0 g) was added with a weak citric acid solution to the formed water-soluble alkaloids salt (Widiyanti et al, 2016). Releasing-alkaloid beetroot powder was then extracted using hydroethanolic (ethanol: water, 70:30) for 3´24 h in a macerator.…”