A challenge for bioprinting tissue constructs is enabling the viability and functionality of encapsulated cells. Rationally designed bioink that can create appropriate biophysical cues shows great promise for overcoming such challenges. Here, a nanoparticle-stabilized emulsion bioink for direct fabrication of porous tissue constructs by digital light processing based 3D bioprinting technology is introduced. The emulsion bioink is integrated by the mixture of aqueous dextran microdroplets and gelatin methacryloyl solution and is further rendered stable by š·-lactoglobulin nanoparticles. After bioprinting, the printed tissue constructs create the macroporous structure via removal of dextran, thereby providing favorable biophysical cues to promote the viability, proliferation, and spreading of the encapsulated cells. Moreover, a trachea-shaped construct containing chondrocytes is bioprinted and implanted in vivo. The results demonstrate that the generated macroporous construct is of benefit to cartilage tissue rebuilding. This work offers an advanced bioink for the fabrication of living tissue constructs by activating the cell behaviors and functions in situ and can lead to the development of 3D bioprinting.
In article number 2102810 by Rui Liu and co-workers, a nanoparticle-stabilized emulsion bioink via integration of two immiscible aqueous phases is developed to directly bioprint macroporous hydrogel constructs. The bioprinted macroporous hydrogel constructs do excellent service to the proliferation, spreading, and maturation of encapsulated cells in vitro and in vivo.
Subperiosteal
implants represent an alternative implant approach
for cases with severe bone atrophy. Although some successful clinical
cases have been reported, the biomechanical stability of subperiosteal
implants remains unclear, and more data are needed to confirm the
feasibility of this approach. Therefore, this study investigated the
biomechanical characteristics of subperiosteal implants based on histological
observation, clinical cases, and finite element analysis. Finite element
analysis indicated that subperiosteal implants with a lattice-like
structure could better disperse the stress to the underlying bone
surface. A novel customized subperiosteal implant was then digitally
designed and fabricated using an additive manufacturing technology.
Six beagle dogs received such customized subperiosteal implants. Histological
and microcomputed tomography examination showed new bone growth into
and around the implant. Patient-specific subperiosteal implants were
placed into the edentulous mandibular bone, with immediate loading.
The implant was functional, without pain or infection, over a 12 month
observation period. Images taken 12 months post-operatively showed
new bone formation and osseointegration of the device. This indicated
that 3D-printed lattice-like subperiosteal implants have sufficient
stability for the rehabilitation of severely atrophic ridges.
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