Optimal conditions for studying phytohemagglutinin(PHA)-induced transformation
of baboon peripheral blood lymphocytes were determined. Maximal stimulation,
as determined by uptake and incorporation of tritiated thymidine (total cell and trichloroacetic-
acid-precipitable radioactivity), occurred at a PHA level of 12.5 μg in a culture
volume of 0.25 ml containing 2 x 10^5 lymphocytes. Optimal stimulation occurred after a
total incubation period of 114 h, the last 18 h of which was in the presence of 1 μCi of
the labeled DNA precursor per culture. While there was considerable variation in the extent
of responsiveness of lymphocytes from individual animals, the shape of the doseresponse
and time-course curves for most mitogen concentrations was generally similar.
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