Because the phosphoenolpyruvate:sugar phosphotransferase system plays multiple regulatory roles in addition to the phosphorylation-coupled transport of many sugars in bacteria, synthesis of its protein components is regulated in a highly sophisticated way. Thus far, the cAMP receptor protein (CRP) complex and Mlc are known to be the major regulators of ptsHIcrr and ptsG expression in response to the availability of carbon sources. In this report, we performed ligand fishing experiments by using the promoters of ptsHIcrr and ptsG as bait to find out new factors involved in the transcriptional regulation of the phosphoenolpyruvate:sugar phosphotransferase system in Escherichia coli, and we found that the anaerobic regulator ArcA specifically binds to the promoters. Deletion of the arcA gene caused about a 2-fold increase in the ptsG expression, and overexpression of ArcA significantly decreased glucose consumption. In vitro transcription assays showed that phospho-ArcA (ArcA-P) represses ptsG P1 transcription. DNase I footprinting experiments revealed that ArcA-P binds to three sites upstream of the ptsG P1 promoter, two of which overlap the CRP-binding sites, and the ArcA-P binding decreases the CRP binding that is essential for the ptsG P1 transcription. These results suggest that the response regulator ArcA regulates expression of enzyme IICB Glc mediating the first step of glucose metabolism in response to the redox conditions of growth in E. coli.The bacterial phosphoenolpyruvate:sugar phosphotransferase system (PTS) 1 consists of two general cytoplasmic proteins, enzyme I and histidine phosphocarrier protein HPr, and some sugar-specific components collectively known as enzyme II (1, 2). Glucose-specific enzyme II of Escherichia coli consists of two subunits, soluble enzyme IIA Glc (EIIA Glc ) and membrane-bound enzyme IICB Glc . Thus, glucose transport in E. coli involves three soluble PTS components (enzyme I, HPr, and EIIA Glc , encoded by the ptsHIcrr operon) and one membranebound protein, enzyme IICB Glc (EIICB Glc ), encoded by the ptsG gene. During translocation of glucose, a phosphoryl group derived from phosphoenolpyruvate is transferred sequentially along a series of proteins to the transported glucose molecule, eventually converting it into glucose 6-phosphate. The sequence of phosphotransfer is from phosphoenolpyruvate to the general PTS proteins enzyme I and HPr and further to the carbohydrate-specific cytoplasmic EIIA Glc , membrane-bound EIICB Glc , and glucose.The PTS takes an important part in metabolic adaptation to environmental changes to compete effectively with ambient organisms by sensing the availability of nutrients in the environment. In addition to sugar transport, multiple roles are exerted by the PTS and these include chemoreception (3), catabolite repression (4), carbohydrate transport and metabolism (1, 5, 6), carbon storage (7,8), and the coordination of carbon and nitrogen metabolism (9). More recently, we found that EIIA Glc of the PTS also regulates the flux between respirat...

Multi-layered material (sandwich panel) consists of double-sided steel plate which is incombustible material or similar material and core material which is not incombustible material. In case of sandwich panel which uses combustible material as insulation, flames spread inside the steel plate at the time of fire so that it is difficult to extinguish fire from the outside and flames spread rapidly and may cause the building to collapse. The current Building Act requires the sandwich panel to secure fire-retardant performance according to the purpose and size of building. In this study, the fire spreading prevention structure applied to partial exterior walls was applied to multi-layered material and its effect was measured through full scale fire test and the possibility to secure fire safety of buildings by applying the fire spreading prevention structure to multi-layered material in future was presented.

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PurposeThe purpose of this research is to explore the ways of integrating situational awareness into business process management for the purpose of realising hyper automated business processes. Such business processes will help improve their customer experiences, enhance the reliability of service delivery and lower the operational cost for a more competitive and sustainable business.Design/methodology/approachOntology has been deployed to establish the context modelling method, and the event handling mechanisms are developed on the basis of event calculus. An approach on performance of the proposed approach has been evaluation by checking the cost savings from the simulation of a large number of business processes.FindingsIn this research, the authors have formalised the context presentation for a business process with a focus on rules and entities to support context perception; proposed a system architecture to illustrate the structure and constitution of a supporting system for intelligent and situation aware business process management; developed real-time event elicitation and interpretation mechanisms to operationalise the perception of contextual dynamics and real-time responses; and evaluated the applicability of the proposed approaches and the performance improvement to business processes.Originality/valueThis paper presents a framework covering process context modelling, system architecture and real-time event handling mechanisms to support situational awareness of business processes. The reported research is based on our previous work on radio frequency identification-enabled applications and context-aware business process management with substantial extension to process context modelling and process simulation.

As one of data mining techniques, outlier detection aims to discover outlying observations that deviate substantially from the reminder of the data. Recently, the Local Outlier Factor (LOF) algorithm has been successfully applied to outlier detection. However, due to the computational complexity of the LOF algorithm, its application to large data with high dimension has been limited. The aim of this paper is to propose grid-based algorithm that reduces the computation time required by the LOF algorithm to determine the k-nearest neighbors. The algorithm divides the data spaces in to a smaller number of regions, called as a “grid”, and calculates the LOF value of each grid. To examine the effectiveness of the proposed method, several experiments incorporating different parameters were conducted. The proposed method demonstrated a significant computation time reduction with predictable and acceptable trade-off errors. Then, the proposed methodology was successfully applied to real database transaction logs of Korea Atomic Energy Research Institute. As a result, we show that for a very large dataset, the grid-LOF can be considered as an acceptable approximation for the original LOF. Moreover, it can also be effectively used for real-time outlier detection.

A sandwich panel is a composite material composed of a double-sided noncombustible material and insulation core which is used in the inner, outer walls, and roof structure of a building. Despite its excellent insulation performance, light weight and excellent constructability, a flame is brought into the inside of the panel through the joint between the panels, melting the core easily and causing casualties and property damage due to the rapid spread of flame. The current Building Law provides that the combustion performance of finishing materials for buildings should be determined using a fire test on a small amount of specimen and only a product that passes the stipulated performance standard should be used. This law also provides that in the case of finishing materials used for the outer walls of buildings, only materials that secured noncombustible or quasi-noncombustible performance should be used or flame spread prevention (FSP) should be installed. The purpose of this study was to confirm the difference between the dangers of horizontal and vertical fire spread by applying FSP, which is applied to finishing materials used for the outer walls of buildings limitedly to a sandwich panel building. Therefore, the combustion behavior and effects on the sandwich panel according to the application of FSP were measured through the construction to block the spread of flame between the panels using a full scale fire according to the test method specified in ISO 13784-1 and a metallic structure. The construction of FSP on the joint between the panels delayed the spread of flame inside the panels and the flash over time was also delayed, indicating that it could become an important factor for securing the fire safety of a building constructed using complex materials.

Sandwich panels which are having the both sides are bonded with a heat insulating material with an iron plate are used as factories, warehouse structures as advantages of convenience in construction at economic efficiency of material cost. However, in a panel structure constructed by continuous joining of sandwich panels, a joint portion where a panel and a panel are connected is generated. The joint part is a part which is easily vulnerable to fire because flames easily flow into the melting and deformation of the iron plate during fire. The flames flowing into the panel induce diffusion of fire by rapid burning, causing damage of human life and property. In this research, we developed a flame spread prevention plate to prevent spreading of sandwich panel. This is an improvement of the workability by the anti-spreading construction method of the existing previous research, it can be applied independently to the connecting part where the panel and the panel are coupled, designed to prevent inflow and spreading of flame did. The actual fire test of the test method of KS F ISO 13784-1 of the sandwich panel specimen was conducted and the burning behavior corresponding to the presence or absence of application of the flame spread prevention plate was grasped at the panel connection part and its effect was measured. Inserting a fire spreading plate into the test result panel connecting part is measured by delaying the flashover, prevention of collapse of the specimen, and temperature rise of the opening, effectively improving the fire safety of the panel structure It was confirmed as a method that can be secured. It is judged that panel structure will contribute to ensuring fire safety by applying the fire spread prevention construction method of various methods ensuring the workability and economy of panel connection vulnerable to fire.