Polymorphism in genes encoding cytokines and proteins involved in lipid metabolism can provide insights into the genetics of the disease and may contribute to assess the associated risk of cardiovascular diseases (CVD), dyslipidemia and metabolic syndrome (MetS) associated with PCOS.

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Direct shoot regeneration was established from leaf, petiole and nodal explants from in vitro and field grown plants of Solanum nigrum on Murashige and Skoog (MS), and Gamborg's (B5) media containing different levels of plant growth regulator, Thidiazuron (TDZ). Comparison of the individual effect of basal media, levels of TDZ, explant type or source of explants on shoot regeneration was tested. Percent shoot regeneration from leaf explant was highest (86.66%) followed by in vitro source of explant (83.65%), B5 basal medium (83.20%) or 2.27 µM TDZ (73.70%) after 60 days of initial culture. The number of shoots was highest (35.10) with 9.13 cm longest shoot at 2.27 µM TDZ. There was a little correlation between predictors and dependent variables was found; whereas, GLM analysis demonstrated significant correlation between the factors. In vitro shoots were transferred to a half-strength MS medium containing different concentrations of NAA or IBA in MS or B5 medium. MS medium produced 100% roots supplemented with 0.5 µM NAA after 28 days. However, stout and hardy rooted shoots were obtained at 0.5 µ IBA with 10.19 mean number and 5.57 cm long roots. Rooted shoots were shifted to ex vitro conditions in polyethylene-cups filled with peat moss: sand: garden soil (1:1:1) with 85.50% survival with true-totype morphology under the natural conditions. The present investigation demonstrated that TDZ may be used for high frequency direct shoot regeneration from leaf explants of S. nigrum on B5 basal medium. This protocol may be useful for subsequent high yielding metabolites producing plants.

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