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Cryopreservation has a key role to achieve a success in insemination of ewes as well as in other animals (Evans, 1988). During the cryopreservation procedure, ram semen gets affected physically (more intense in rams than bull), biochemically and functionally. After the freeze-thaw process of ram semen, about 40%-60% of the spermatozoa maintain the motility. However, only about 20%-30% stay unharmed (Salamon & Maxwell, 2000).Cryopreservation's harmful effects mainly result from ice formation in cells and lipid peroxidation (Fayyaz et al., 2017;Pesch & Bergmann, 2006). Lipid peroxidation leads to the formation of reactive oxygen species (ROS), and the ROS level increases with incubation due to cellular respiration. As there are many harmful agents present, reduction in viability, motility, integrity of acrosome and