Freeze-dried egg yolk based extenders containing various antioxidants improve post-thawing quality and incubation resilience of goat spermatozoa

Alçay, Selim; Gökçe, Elif; Toker, Mehmed Berk; Onder, N. Tekin; Üstüner, Burcu; Uzabacı, Ender; Gül, Zülfiye; Cavus, Seda

doi:10.1016/j.cryobiol.2016.03.007

Cited by 35 publications

(44 citation statements)

References 30 publications

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“…This could be originated from our base extender include cysteine and this already have an antioxidant capacity. Our results agreed with previously published studies (3,14,24) Demircan et al (17) recently reported that incubating H2O2 and DCFH-DA in oxygenated physiological medium causes a significant increase in fluorescence intensity, and antioxidants can attenuate this increment. Thus, the effectiveness of antioxidant compounds against the H2O2-induced increase in fluorescence intensity was also tested in the medium.…”

Section: Discussionsupporting

confidence: 83%

“…Therefore, it is a key product of lipid peroxidation in the cells (3,35). GSH is a multifunctional non-protein which is known as a thiol group antioxidant in mammalian cells (12,33,36).…”

Section: Discussionmentioning

confidence: 99%

“…Determination of oxidative stress markers: The MDA levels of each group were measured according to the thiobarbituric acid method (3,35). For this purpose, thiobarbituric acid solution (0.67%) was added to supernatant in a glass tube with 2:1 volume and then tubes were hold at 100 °C for 60 min.…”

Section: Methodsmentioning

confidence: 99%

“…Lipid peroxidation and its final product Reactive Oxygen Species (ROS) are other limiting factors in semen preservation (1,3,27). Their depredatory effects can be specified as irreversible reduction on motility and viability of spermatozoa, and moreover, permanent damages on plasma membrane, acrosome and DNA (11,12).…”

Section: Introductionmentioning

confidence: 99%

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Positive effect of BSA supplemented soybean lecithin based extender on liquid storage of ram semen at 5C

ALÇAY

2017

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:The aim of the study was to evaluate different BSA doses in soybean-lecithin based extenders for liquid storage of ram semen. Pooled semen was divided into four equal volumes and diluted in a single-step dilution method with BSA supplemented (BSA5, BSA7.5, BSA10 groups supplemented 5mg/mL, 7.5mg/mL, 10mg/mL BSA respectively) and BSA-free (control) extenders. Semen samples were assessed for sperm motility, plasma membrane functional integrity using hypo-osmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) at 0, 24, 48 and 72h of storage at 5 °C. Antioxidant activities of groups at 0 and 72h were measured with glutathionine (GSH) concentration, membrane lipid peroxidation status was analyzed using the malondialdehyde (MDA) concentration. Antioxidant capacity of extender also was measured with 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assays. In the study, BSA (5mg/mL) supplementation has beneficial effect on ram sperm parameters at all evaluation times. In addition, the increasing doses of BSA supplementation has a negative effect on sperm parameters especially 48 and 72h of storage.Keywords: BSA, lecithin, ram semen, short-term storage.BSA ilave edilmiş lesitin bazlı sulandırıcının koç spermasının 5 C sıcaklıkta muhafaza edilmesine olumlu etkisi Özet: Bu çalışmada, farklı dozlarda BSA ilave edilmiş lesitin bazlı sulandırıcının koç spermasının 5°C'ta kısa sureli saklanması üzerindeki etkisinin değerlendirilmesi amaçlandı. Çalışma amacıyla alınan spermalar pooling yapılarak dört eşit hacme bölündü ve 5mg/mL, 7.5mg/mL, 10mg/mL BSA ilave edilmiş (BSA5, BSA7.5, BSA10 sırasıyla) ve edilmemiş (kontrol) sulandırıcılarla sulandırıldı. Sulandırılmış spermanın sıcaklığı 5°C'a düştükten sonra 0, 24, 48 ve 72. saatlerde; motilite, plazma membran fonksiyonel bütünük [hipoozmotik şişme testi (HOST)] ve akrozomal hasar [FITC-Pisum sativum agglutinin (PSA-FITC)] muayeneleri yapıldı. Çalışmanın başlangıcı ve sonunda antioksidan aktivitenin ve lipid peroksidasyonun değerlendirilmesi için malondialdehyde (MDA) ve glutathionine (GSH) konsantrasyonları ölçüldü. Ayrıca 2,2-Diphenyl-1-picrylhydrazyl (DPPH) ve Dichloro-dihydro-fluorescein diacetate (DCFH-DA) yöntemleri ile her bir sulandırıcının antioksidan kapasiteleri değerlendirildi. Yapılan muayenelerin sonucunda; 5mg/mL BSA ilavesinin koç spermasının kısa süreli muhafazasında etkili olduğu gözlemlendi. Ayrıca artan BSA konsantrasyonunun spermanın soğutularak saklanmasında olumsuz etki oluşturduğu belirlendi. Anahtar sözcükler: BSA, kısa süreli muhafaza, koç sperması, lesitin.

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Section: Discussionsupporting

confidence: 83%

“…Therefore, it is a key product of lipid peroxidation in the cells (3,35). GSH is a multifunctional non-protein which is known as a thiol group antioxidant in mammalian cells (12,33,36).…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Positive effect of BSA supplemented soybean lecithin based extender on liquid storage of ram semen at 5C

ALÇAY

2017

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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“…These undesirable effects may cause irreversible decrease on sperm function parameters (Nur et al, 2010; Ustuner et al, 2016). The success of sperm cryopreservation depends on the composition of the extenders which is a crucial factor to protect spermatozoon (Alcay, Gokce, et al, 2016; Toker, Alcay, Gokce, & Ustuner, 2016).…”

Section: Introductionmentioning

confidence: 99%

Goat semen cryopreservation with rainbow trout seminal plasma supplemented lecithin‐based extenders

et al. 2020

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The objective of this study was to determine the optimum concentrations of rainbow trout seminal plasma (RTS) supplemented extenders for goat semen quality at post‐thaw and after incubation. Five sexually mature Saanen goat (Capra aegagrus hircus) were used for semen collection. Pooled semen was diluted with soybean lecithin‐based extender without RTS (control) or supplemented with different concentrations of RTS (1%, 2%, 4% or 8%), at a final concentration of 150 × 106 spermatozoon/ml. Sperm motility, plasma membrane functional integrity (HOST), damaged acrosome (PSA‐FITC), mitochondrial activity (rhodamine123) and DNA integrity (TUNEL) were evaluated. Spermatological parameters were evaluated at post‐thaw and after 6 hr incubation. RTS8 group preserved sperm motility, acrosomal integrity, plasma membrane functional integrity and mitochondrial function better than the control group (p < .05). The study demonstrated that RTS supplemented lecithin‐based extenders have useful effects on goat spermatozoa. In addition, the results of the current study represented the positive effect of using 8% RTS supplemented extender.

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Methionine, cysteine, and butylated hydroxytoluene enhance cryosurvival of ram semen on post-thaw and post-incubation time points

Toker

Doğan

2023

Trop Anim Health Prod

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Freeze-dried egg yolk based extenders containing various antioxidants improve post-thawing quality and incubation resilience of goat spermatozoa

Cited by 35 publications

References 30 publications

Positive effect of BSA supplemented soybean lecithin based extender on liquid storage of ram semen at 5C

Positive effect of BSA supplemented soybean lecithin based extender on liquid storage of ram semen at 5C

Goat semen cryopreservation with rainbow trout seminal plasma supplemented lecithin‐based extenders

Methionine, cysteine, and butylated hydroxytoluene enhance cryosurvival of ram semen on post-thaw and post-incubation time points

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