This randomised study aimed to compare the biochemical tolerance of three parenteral regimens administered during the first 48 hours of life. Twenty nine infants were randomised to either: (a) glucose 10%; (b) glucose 100/o/amino acids; (c) glucose 100/olamino acids/lipid.

This study aimed to investigate energy substrate utilisation and nitrogen balance in low birthweight infants receiving total parenteral nutrition during the first days of life, and in particular, to determine the effect of two different glucose intakes on carbon dioxide production. Twenty infants (mean (SE) birthweight 1314 (65) g, mean (SE) gestation 30 9 (0.4) weeks) were recruited to the study. Immediately after birth they were randomised to a carbohydrate intake of 8 glkglday (5.5 mg/kg/minute) or 12 g/kg/ day (8.3 mg/kg/minute). After 24 hours they were changed to the alternative regimen which was continued for a further 24 hours. Fat and protein intakes were kept constant throughout the study. Indirect calorimetry was performed during each of the regimens, urine was coliected for urinary nitrogen, and substrate utilisation calculated for 12 infants.The carbohydrate utilisation rate was increased during the higher carbohydrate intake. Lipid utilisation rates were significantly different, with net lipid synthesis occurring during high carbohydrate intake. Protein utilisation rates were not influenced by the different carbohydrate intakes. The mean plasma glucose concentration was higher during the high carbohydrate intake but the mean highest and lowest values were not significantly different during the two study periods. A plasma glucose below 2-6 mmol/l was recorded more frequently during the low glucose intake (9/20 v 5/20). Capillary Pco2 values measured during high and low glucose intakes were similar (5.9 (0.2) v 6-2 (0.3) kPa. Carbon dioxide production rates were increased during the higher carbohydrate intake but the differences were not significant. Similarly, there was no significant difference in the respiratory quotients (RQ), oxygen consumption, or energy expenditure during the two study periods. (Arch Dis Child 1995; 73: F13-F16)

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ContextThe high incidence of lamb mortality in Merino sheep is a persistent issue for the industry. Poor-quality colostrum or inadequate colostrum consumption increases the risk of low energy intake and failed passive immunity transfer, resulting in lamb mortality. The rapid assessment of colostrum quality and intake would be a valuable tool for producers which house ewes for lambing.AimsThis experiment determined the relationship and accuracy of analysis techniques to measure immunoglobulin G (IgG) and total protein (TP) concentrations within ewe colostrum and lamb serum and compared those results against digital hand-held refractometers.MethodsMerino ewe colostrum and lamb blood were sampled at 4 and 24 h post-partum during autumn (n=93 ewes) and spring (n=79 ewes) in 2018. Colostrum was analysed for total milk solids (%) and lamb serum was analysed for total blood serum protein (%), and both colostrum and lamb serum were analysed for IgG and TP using in-house laboratory analysis techniques. Correlation coefficients were performed to identify the relationship between the different assessment methods.Key resultsThere was a significant Pearson’s correlation (P<0.05) between 4 h total milk solids and TP (R=0.19), between total milk solids and IgG (R=0.19), and TP and IgG (R=0.38). For 24 h colostrum samples, the significant correlations (P<0.001) between TP and IgG (R=0.54) and total milk solids and IgG (R=0.56) were higher than the correlation between total milk solids and TP (R=0.24; P=0.006). Further, 4 h serum IgG (R=–0.14; P=0.026) and 24 h total blood serum protein (R=0.21; P=0.009) were correlated with lamb survival to 72 h.ConclusionsWhile the refractometer provided a crude measure of colostrum IgG and TP and was valuable in identifying lambs with low IgG transfer at 24 h, further research is required regarding analysis techniques to allow for reliable measures need to be refined.ImplicationsThe use of refractometers to assess colostrum quality and intake could benefit researchers or producers who practice indoor lambing.

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