This study demonstrates that binding of activated platelets occurs to monocytes and neutrophil granulocytes but not to T- and B-cells in the circulation after transfusion. In addition, the interaction of platelets and WBCs is dependent on the degree of P-selectin expression. Platelets showing a higher degree of activation adhere to WBCs to a higher degree than nonactivated platelets.
Background and Objectives: In this study we investigated whether platelet activation during apheresis results in the binding of platelets to white blood cells. Material and Methods: Analysis of platelet–leukocyte interaction was performed using multiparameter, three–color flow cytometry. Results: Over the duration of the procedure, there was an increase in the surface expression of CD62p (P–selectin) and CD63 (p<0.05), and also in the binding of platelets to monocytes (p<0.05), neutrophilic granulocytes (p<0.05) and to CD3+ cells (initially to a low degree; p<0.05). Platelet binding to CD19+ cells did not change significantly. Conclusion: This study demonstrates that platelets become activated during apheresis and that following this process, interaction with monocytes and neutrophilic granulocytes occurs.
This study demonstrates that platelets become activated during apheresis and that following this process, interaction with monocytes and neutrophilic granulocytes occurs.
Background: Long-term plateletpheresis has been suspected to cause depression in cell-mediated immunity. To prove this hypothesis, we analyzed blood samples by flow-cytometric immunophenotyping in plateletpheresis donors over a period of 26 months. Methods: Donors were included beginning with their first donation (n = 20). A non-donor control group was matched for age and sex (n = 20). Samples were taken before each apheresis and at 3-month intervals (control group: ± 2 days), and analyzed by flow cytometry. A minimum of 6 procedures per year (range 6–14) was the precondition for each donor to be included in the study. Results: No significant differences were seen within the two groups regarding the absolute white blood cell count, total lymphocyte count, relative and absolute counts of CD3+ (p = 0.82), CD4+ (p = 0.91), CD8+ (p = 0.74), CD4:CD8 ratio (p = 0.65), CD16+56+ (p = 0.54), CD25+ (p = 0.82) and CD3+/HLA-DR+ cells (p = 0.66). Conclusions: Long-term plateletpheresis does not seem to alter the absolute white blood cell count, total lymphocyte count or lymphocyte subsets in donors. Regarding the cells and subsets analyzed, plateletpheresis is a safe procedure and does not seem to have a detrimental effect on the cellular immune competence.
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