Extracorporeal Plateletpheresis Induces the Interaction of Activated Platelets with White Blood Cells

Gutensohn, K.; Alisch, A.; Krueger, W.; Kroeger, N.; Kuehnl, P.

doi:10.1046/j.1423-0410.2000.7820101.x

Cited by 19 publications

(4 citation statements)

References 37 publications

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“…These interactions may activate platelets moreover contaminating small amount of endotoxin which occurs in PCs as storage time increase. [ 3 9 10 11 12 ] Can activate many types of cells in the concentrate to produce RANTES.…”

Section: Discussionmentioning

confidence: 99%

“…Gutensohn et al . [ 11 ] demonstrated that in apheresis using Amicus, there is activation of platelets and there is interaction with monocytes and granulocytes. After the preparation of SDP with Amicus, lots of big aggregates was found, this finding was also observed by Hagberg et al .,[ 13 ] who showed that platelets from Amicus have increased fibrinogen receptor activation, increased the size of platelet aggregates, increased the formation of microparticles, increased degranulation, decreased von Willebrand factor receptor surface expression, and also decreased responses to in vitro agonist stimulation.…”

Section: Discussionmentioning

confidence: 99%

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Regulated upon activation, normal T cells expressed and secreted (CCL5) in platelet concentrate: Role of mode of preparation and duration of storage

Shukla

Mody

Gupte

et al. 2021

Asian J Transfus Sci

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BACKGROUND AND OBJECTIVES: Platelet concentrates (PCs) can be prepared in several different ways, and they can be stored over few days before the use. Regulated on activation, normal T cells expressed and secreted (RANTES) levels in these concentrates may vary depending on the type of preparation and duration of storage of this component. We measured RANTES levels in platelet supernatants in different preparations and with different storage duration. MATERIALS AND METHODS: Fifteen PCs were prepared by platelet-rich plasma (PRP) and buffy coat (BC) method each. Forty-two single donor platelets (SDPs) were prepared using cell separators Cobe Spectra, Trima Accel, and Amicus. Filtered PCs were prepared using labside and bedside filters. The supernatants were collected after 1, 18, 65, and 112 h of preparation. SDP samples were taken on the 0 day, 3 rd day, and 5 th day. In filtered PC, pre- and post-filtration samples were taken, and aliquots were frozen at − 56°C for the measurement of RANTES. RESULTS: RANTES at 1 h was 1210 ± 560 pg/ml in PRP-PC, 1384 ± 463 pg/ml in BC-PC. At 112 h, 1617 ± 451 pg/ml and 1949 ± 134 pg/ml, respectively. In SDP, 0-day level was 1850 ± 278 pg/ml and >2000 pg/ml on 5 th day. In prestorage, filtered PC RANTES was 1035 ± 496 pg/ml, and in the poststorage sample, it was 310 ± 508 pg/ml. With bedside filters, presample showed 1243 ± 832 pg/ml and postsample showed 556 ± 748 pg/ml. CONCLUSION: The concentration of RANTES increased continuously from 1 h to 5 days of storage in all PCs. After 65 h, BC-PC showed higher levels of RANTES compared to PRP-PC. Filtered PRP-PCs appear to be the best in terms of low RANTES to prevent allergic reactions and cultures negative.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Regulated upon activation, normal T cells expressed and secreted (CCL5) in platelet concentrate: Role of mode of preparation and duration of storage

Shukla

Mody

Gupte

et al. 2021

Asian J Transfus Sci

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“…The procedure causes leukocyte activation, inducing an increase in plasma tumor necrosis factor (TNF)-alpha levels; however, this has not been shown to impair endothelial cell function [55]. In addition, platelets become activated during apheresis and have been shown to interact with leukocytes [56]. Contact of platelets with artificial surfaces induces transient increases in platelet-derived soluble growth factors [57].…”

Section: Discussionmentioning

confidence: 99%

The role of thrombocytapheresis in the contemporary management of hyperthrombocytosis in myeloproliferative neoplasms: A case-based review

et al. 2017

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Extreme thrombocytosis induces an acquired thrombotic-hemorrhagic diathesis, and left uncontrolled is a harbinger of potentially fatal vascular complications. Currently, cytoreduction with medical therapy remains the mainstay of hyperthrombocytosis management. However, it offers a less-than-ideal option in situations where a rapid reduction in platelets is urgently needed, as in the presence of vital end-organ ischemia or to ameliorate of life-threatening hemorrhage. The role of thrombocytapheresis, or plateletpheresis, in hyperthrombocytosis has become increasingly obsolete given the proactive titration of cytoreductive therapies and early identification and correction of reversible causes of reactive thrombocytosis. Despite its narrowed indications, plateletpheresis continues to offer a valuable temporizing measure in platelet count reduction before cytoreductive agents exert their maximal effect. In this context, it is important for the treating physician to be aware of the symptoms and risks associated with hyperthrombocytosis to inform best clinical practices. In this review, we discuss the role of plateletpheresis in the modern-day management of hyperthrombocytosis in patients with myeloproliferative neoplasms through a case based review of the literature. It becomes apparent throughout the discussion that the decision to perform plateletpheresis should be individualized based upon the clinical scenario, degree of thrombocytosis, available infrastructure and every patient’s risk profile.

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“…This function is attributed to professional phagocytes equipped with specific receptors for PS, such as the class B scavenger proteins SR‐ BI and CD36 (10–13). This phenomenon means that platelets with a high PS content in the outer plasma membrane could be removed after transfusion by the reticuloendothelial system independently of their functional status (10,11,14,15). These events may play an important role in the removal of senescent platelets from the circulation (12,13).…”

Section: Introductionmentioning

confidence: 99%

Platelet Function, Activation and Apoptosis During and After Apheresis

et al. 2010

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Platelets are known to undergo shape change, activation, release reaction and apoptosis/necrosis during processing and storage. Apheresis may have a deleterious impact on platelet achievability and functional integrity. Platelet concentrates from 50 male volunteers obtained by COBE spectra were screened for platelet activation (CD62 and CD154) and apoptosis (phosphatidylserine detected by Annexin V). Donor samples before separation, during apheresis and at the third day of storage were used as baseline donor samples. Platelet aggregation to adenosine diphosphate (ADP) and collagen was performed. There was a statistically significant increase in the expression of activation markers in two different samples (during separation samples and third day samples). Although the increase in Annexin V expression was not so observable, it showed a significant increase also. There was marked decline in the platelet aggregation. The correlations between the values of CD62, CD154 and Annexin V were detected in baseline samples and increased during separation and at the third day of platelets storage. Correlation between values of platelet aggregation to collagen and Annexin V was relevant only in the baseline samples. No other correlations were encountered between platelet aggregation and markers of activation and apoptosis during apheresis and storage. Initial platelet activation induced by apheresis may have an impact on phosphatidylserine expression with no impact on aggregation function of platelets during storage.

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Extracorporeal Plateletpheresis Induces the Interaction of Activated Platelets with White Blood Cells

Abstract: This study demonstrates that platelets become activated during apheresis and that following this process, interaction with monocytes and neutrophilic granulocytes occurs.

Cited by 19 publications

References 37 publications

Regulated upon activation, normal T cells expressed and secreted (CCL5) in platelet concentrate: Role of mode of preparation and duration of storage

Regulated upon activation, normal T cells expressed and secreted (CCL5) in platelet concentrate: Role of mode of preparation and duration of storage

The role of thrombocytapheresis in the contemporary management of hyperthrombocytosis in myeloproliferative neoplasms: A case-based review

Platelet Function, Activation and Apoptosis During and After Apheresis

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