Platelet function testing in apheresis products: flow cytometric, resonance thrombographic (RTG) and rotational thrombelastographic (roTEG) analyses

Gutensohn, K.; Geidel, K.; Kroeger, N.; Eifrig, B.; Crespeigne, N.; Kuehnl, P.

doi:10.1016/s1473-0502(02)00007-1

Cited by 19 publications

(22 citation statements)

References 38 publications

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“…8,9 In addition, a complementary analysis of platelet quality enables us to confirm that a higher centrifugation speed may severely alter platelet functionality. This fact was evidenced by the occurrence of platelet autoaggregation, reduction of platelet response to agonist, and transition from a resting to an activated morphology visualized by electron microscopy.…”

Section: Discussionmentioning

confidence: 99%

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Formulation and Storage of Platelet-Rich Plasma Homemade Product

Bausset¹,

Guy²,

Véran³

et al. 2012

BioResearch Open Access

113

116

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The platelet-rich plasma (PRP) is an autologous biotherapy based on platelet-healing properties. Here, we developed a simple and reproducible PRP purification protocol based on two successive centrifugations. We evaluated different centrifugation speeds and time-storage durations on the platelet quantity and quality. Sterility and stability of our PRP homemade product were also performed. We prepared PRP from 54 healthy volunteers. We tested activation state, reactivity, and stability of platelets by flow cytometry using basal and adenosine diphosphate (ADP)-induced P-selectin expression markers; growth factor release after platelet activation by an enzyme-linked immunosorbent assay (ELISA); platelet aggregation capacity by aggregrometry assays; clot formation and retraction by thromboelastography; and platelet morphology by ultrastructural analysis. About 130 and 250 g successive speed centrifugations further concentrated platelets while preserving their bioactivity during 6 h (after that, platelet functions were significantly altered). In these conditions, we obtained a highly concentrated pure PRP product (with a low leukocyte count) suitable to study platelet properties. To avoid the loss of efficacy, we recommend injecting PRP under 3 h after preparation.

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Section: Discussionmentioning

confidence: 99%

“…Regarding analysis of platelet concentrations, a short R, a short k, and a high MA demonstrate a good reaction of platelets, their ability to initiate a clot, and also their activation. 9 …”

Section: Methodsmentioning

confidence: 99%

Formulation and Storage of Platelet-Rich Plasma Homemade Product

Bausset¹,

Guy²,

Véran³

et al. 2012

BioResearch Open Access

113

116

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“…Various studies have investigated platelet activation, which has been used to evaluate platelet quality during processing and storage of plateletpheresies products. Activated platelet markers that have been used in studies of plateletpheresis include P-selectin (CD62P) [2,27,30], CD63 [20,22,47], gly- [12,13,19,28,62], and coated platelets [1,11,57]. Trima Accel: this suggests that increased P-selectin expression may result from delayed processing times within the apheresis device [27,30].…”

Section: Platelet Activationmentioning

confidence: 99%

“…It is considered to be a platelet activation marker [23]. Gutensohn et al [22] reported that the CD63 levels of platelets collected using Amicus, Cobe Spectra, and Trima devices increased after storage for 5-8 days [20,47].…”

Section: Platelet Activationmentioning

confidence: 99%

Plateletpheresis: the Process, Devices, and Indicators of Product Quality

Jang¹,

Kim²,

Kim³

et al. 2014

Journal of Life Science

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Platelet products are used to treat hemorrhagic or platelet dysfunction diseases. Plateletpheresis involves collecting the platelet components of blood using an apheresis blood-collection system. Various indicators are available for evaluating the qualities of the apheresis platelets. The productivity of platelet collection is evaluated through both the collection efficiency and collection rates. Platelet storage quality can be evaluated in vitro using several indicators, including visual appearance, metabolic activities, volume, platelet count, white blood cell count, microparticles, and various platelet activation markers. Platelet activation markers have been used as indicators of storage quality in various studies. Post-transfusion platelet quality can be evaluated based on the corrected count increment and the percentage of platelet recovery. Although various studies have investigated the aspects of plateletpheresis, no article has systemically presented assessments of the platelet products obtained from different plateletpheresis devices. The present study provides a review of plateletpheresis, including the specifics of the process, the types of devices employed, the platelet quality, the overall efficacy, and the evaluation indicator qualities. Furthermore, the differences in functionality among the different apheresis devices are discussed. Although adverse reactions to the citrate anti-coagulant have been reported, apheresis processing may provide a safer option for donors who are at a high risk for presyncopal or syncopal reactions related to whole blood collection.

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“…Thromboelastography (TEG) is a technique that has been used predominantly in surgical patients to demonstrate the kinetics of clot formation and dissolution and to document the viscoelastic properties of the clot [5,6]. It may be used to assess abnormalities in clot production and stability and may help determine their cause.…”

Section: Introductionmentioning

confidence: 99%

Storage of Blood Components Does Not Decrease Haemostatic Potential: In vitro Assessment of Fresh versus Stored Blood Components Using Thromboelastography

Bartfeld

Ellis²,

Lubetzky³

et al. 2010

Transfus Med Hemother

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Background: Major surgery and severe trauma typically lead to massive blood loss requiring rapid transfusion of large amounts of blood products. It has been suggested that fresh, unrefrigerated whole blood provides a haemostatic advantage in this setting. The aim of the current study was to compare the clot formation parameters of fresh, unrefrigerated whole blood and whole blood reconstituted from components stored for varying periods of time, using rotational thromboelastography (ROTEM®). Methods: Fresh whole blood and reconstituted whole blood using combinations of nonleucoreduced red cell units (stored for 7, 14, 21, 28, or 35 days), platelet concentrates (stored for 1, 3 or 5 days), and fresh frozen plasma (stored for 6 months) were analysed using ROTEM. Measurements of the clotting time (CT), clot formation time (CFT), and maximal clot firmness (MCF) were compared between units of fresh whole blood and reconstituted whole blood samples. Results: There was no difference in the haemostatic parameters measured of fresh whole blood and reconstituted whole blood using red cell units stored for less than 21 days. ROTEM demonstrated that the CT and CFT were significantly shorter for reconstituted whole blood samples using red cells stored for longer than 21 days when compared to fresh whole blood and to reconstituted whole blood samples using red cell units stored for less than 21 days. The CT was inversely correlated to the duration of platelet storage. The MCF was unchanged regardless of duration of blood product storage. Conclusion: Fresh unrefrigerated whole blood and blood products stored for short duration (less than 21 days) were not superior to those stored for longer durations.

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Platelet function testing in apheresis products: flow cytometric, resonance thrombographic (RTG) and rotational thrombelastographic (roTEG) analyses

Cited by 19 publications

References 38 publications

Formulation and Storage of Platelet-Rich Plasma Homemade Product

Formulation and Storage of Platelet-Rich Plasma Homemade Product

Plateletpheresis: the Process, Devices, and Indicators of Product Quality

Storage of Blood Components Does Not Decrease Haemostatic Potential: In vitro Assessment of Fresh versus Stored Blood Components Using Thromboelastography

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