In order to study the effect of feeding non-starch polysaccharides (NSP) on the production and absorption of the malodorous compounds skatole and indole, eighteen male pigs, representing nine littermate groups, were used. One pig from each litter was assigned to either a low-NSP diet (87 g/kg of NSP) or a high-NSP diet (160 g/kg of NSP). Faecal samples and blood samples from vena jugularis were collected during a three-day period. The production of indolic compounds in different sections of the gastro-intestinal tract of six littermate groups was measured at slaughter (after 1 month on the diets). To investigate the absorption of indole and skatole, three catheterized pigs, acting as their own control (2 weeks on each diet) were used. Parallel determinations in portal blood, peripheral blood and in faeces of skatole and indole were conducted. Faecal, intestinal and blood samples were analysed for indolic compounds by high-performance liquid chromatography (HPLC). The production of indole and skatole in the proximal and distal part of the hind gut, respectively, was significantly reduced in pigs given the high-NSP diet. Similarly, both blood and faecal samples revealed that dietary NSP-inclusion reduced skatole concentration, whereas a dietary effect of NSP on the indole concentration was reflected in blood samples only. The absorption of skatole and indole was significantly lower in pigs given the high-NSP diet compared with those offered the low-NSP diet. The skatole concentrations in blood and faeces were highly correlated when measured within the individual animal, suggesting that a proportional amount of the skatole produced was absorbed. In contrast, only weak correlations were demonstrated when determined between animals. This emphasizes the great impact that individual hepatic clearance rate would have on the level of skatole in backfat, and consequently the importance of applying cross-over designs, when studying the absorption of indolic compounds.
Short-chain fatty acids (SCFA), viz. acetate, propionate and butyrate are quantitatively important substrates in ruminant energy metabolism. In the reviewed literature, 16 44% of ME intake was recovered as portal appearance of SCFA. This is considerably lower than expected when related to the estimated intragastric flux of SCFA. The discrepancy is caused by portal drained viscera metabolism of arterially abundant metabolites e.g., acetate and the metabolism of acetate and butyrate to acetoacetate and D-3-hydroxybutyrate in the absorptive epithelia. Even though considerable variations between experiments on acetate and propionate appearance are found, there seems to be a great deal of evidence that the proportion of gastrointestinally produced acetate and propionate absorbed to the portal blood is 50-75%. The portal recovery of butyrate has been found to be between 10 and 36% dependent on intraruminal infusion rate. It is concluded that major parts of acetate and propionate are directly absorbed to the portal vein. The true absorption rate of acetate can only be estimated by taking the portal drained viscera metabolism of arterial acetate into account. Butyrate is generally found to have a low recovery in the portal vein, but the production of D-3-hydroxybutyrate seems to be underestimated in major parts of the literature. It is therefore necessary to measure portal appearance as well as portal drained viscera metabolism to assess the quantitative as well as the qualitative contribution of SCFA and SCFA metabolites to whole animal metabolism.
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