A site-specific, stimuli-responsive nanocarrier has been synthesized by conjugating folate, magnetic particles and doxorubicin to the backbone of norbornene polymer. Monomers, namely, cis-5-norbornene-6-(diethoxyphosphoryl)hexanote (mono 1), norbornene grafted poly(ethyleneglycol)-folate (mono 2), and norbornene derived doxorubicin (mono 3) are carefully designed to demonstrate the smart nanorcarrier capabilities. The synthesis and complete characterization of all three monomers are elaborately discussed. Their copolymerization is done by controlled/living ring-opening metathesis polymerization (ROMP) to get the triblock copolymer PHOS-FOL-DOX. NMR spectroscopy and gel permeation chromatography confirm the formation of the triblock copolymer, while FT-IR spectroscopy, thermogravimetric analysis, along with transmission electron microscope confirm the anchoring of iron particle (Fe3O4) to the PHOS-FOL-DOX. Drug release profile shows the importance of having the hydrazone linker that helps to release the drug exactly at the mild acidic conditions resembling the pH of the cancerous cells. The newly designed nanocarrier shows greater internalization (about 8 times) due to magnetic field. Also, increased intracellular DOX release is observed due to the folate receptor. From these results, it is clear that PHOS-FOL-DOX has the potential to act as a smart nanoreservoir with the magnetic field guidance, folate receptor targeting, and finally pH stimulation.
Biocompatible nanocarriers conjugated with magnetic nanoparticle, doxorubicin and poly(ethylene oxide) (PEG) motif have been designed (PVLPEG-PVLDOXI-PCL-PHOS) to create a magnetic vector under magnetic field. Acylhydrazine linker is used to release the drug exactly at the mild acidic conditions resembling the pH of the cancerous cells. All the monomers and polymers are characterized carefully by the routine analytical techniques. Thermogravimetric analysis (TGA), FT-IR spectroscopy and scanning electron microscope (SEM) techniques are employed to confirm the anchoring of iron particle (Fe 3 O 4 ) to the PVLPEG-PVLDOXI-PCL-PHOS. Reservoir capabilities of the newly designed biodegradable nanocarrier are tested by both dynamic light scattering (DLS) and transmission electron microscopy (TEM). Drug release profile from nanocarrier is monitored by fluorimeter. The release profile shows the importance of having the acylhydrazine linker that helps to release the drug at the mild acidic conditions similar to cancerous cells. Confocal laser scanning microscopy (CLSM) and flow cytometry studies on 4T cells indicate that nanocarriers from PVLPEG-PVLDOXI-PCL-PHOS polymer are internalized efficiently. It is very interesting to note that the nanocarriers have exhibited both biologically and magnetically targeting abilities toward 4T cells in vitro.
Pendent functionalization of biodegradable
polymers provides unique
importance in biological applications. In this work, we have synthesized
a polymeric nanocarrier for the controlled release of the anticancer
drug doxorubicin (DOXI). Inspired by the pH responsiveness of acylhydrazine
bonds along with the interesting self-assembly behavior of amphiphilic
copolymers, this report delineates the development of a PEG-SS-PCL-DOXI copolymer consisting of DOXI, PEG, and a caprolactone backbone.
First, the inclusion of a PEG moiety in the copolymer helps to achieve
biocompatibility and aqueous solubility as well as a prolonged circulation
time of the nanocarrier. Second, an acid-sensitive acylhydrazine-based
linkage is chosen to attach DOXI to trigger sustained drug release,
whereas the inclusion of an enzymatically cleavable disulfide linkage
in the backbone adds to the advantage of backbone biodegradability
at the intracellular level.
Poly(ethylene glycol) functionalized with tetra-acetylene (PTETACT) and pentaerythritol (3-mercaptopropionic acid) (PETM) are cross-linked by a thiol–yne reaction to create robust, tuneable networks.
The newly developed polymeric nanocarrier could open a new avenue for cancer therapy, due to its unique design as well as, most importantly, its biocompatible and biodegradable nature.
The first example of simple nanoaggregates, self-assembled from a new class of polymer has been explored. The synthesis and characterization of polyethylene glycol and doxorubicin attached to a 1,6-heptadiyne derivative (Macromonomer) are clearly described. Macromonomer is polymerized via olefin metathesis living cyclopolymerization method using the Grubbs-Hoveyda catalyst to produce a water-soluble Dox-Peg-Rcp-Fmoc polymer. All of the monomers and polymers are carefully characterized by GPC and 1 H NMR spectroscopy. Fmoc deprotection of the Dox-Peg-Rcp-Fmoc polymer is carried out to produce the Dox-Peg-Rcp polymer. The newly designed Dox-Peg-Rcp polymer shows nanoaggregation in water. In addition to that, they are soluble in water as well as biological media. The drug release profile at mild acidic condition shows the importance of having ester linker. CLSM images of Dox-Peg-Rcp nanoaggregates clearly show the efficient internalization into the living cells. MTT assays of Dox-Peg-Rcp nanoaggregates show that these nanoaggregates have a greater anticancer efficacy. To the best of our knowledge, this is the first report on water-soluble polyacetylenes for biological/drug delivery applications.
A pH-responsive, multiple chemotherapeutic agent derived nanocarrier has been synthesized by conjugating doxorubicin, indomethacin, and folate to the backbone of norbornene polymer. Drug molecules are connected to the norbornene backbone by an ester linker to demonstrate the pH-responsive capabilities. The complete chemical and biological properties of the new norbornene-based polymeric nanocarrier, intended for combination cancer chemotherapy, are discussed.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.