Euphorbia tirucalli (E. tirucalli) is a tropical and subtropical plant that produces a latex which is used for several purposes. The components of E. tirucalli latex include triterpenes, diterpenes and steroids. The aim of the present study was to evaluate the effects of diluted E. tirucalli latex on murine B16/F10 melanoma cells and lung metastasis. For this purpose, an in vitro study was first performed, in which B16/F10 cells were treated with diluted (1/2 to 1/11,192) E. tirucalli latex. In a second study, B16/F10 melanoma cells were inoculated into the tail vein of mice to generate lung metastases; the mice then received 0.467 µg of latex diluted in 200 ml saline by gavage for 14 days. A significant decrease in B16/F10 cell viability was observed using the MTT assay at 24 and 48 h after treatment with E. tirucalli latex. In addition, a significant decrease in the volume fraction occupied by B16/F10 metastatic colonies in the lungs was observed in mice treated with E. tirucalli latex. These results confirm the antineoplastic effects of diluted E. tirucalli latex.
Euphorbia tirucalli Lineu (Euphorbiaceae) is a tropical and subtropical ornamental and toxic plant. E. tirucalli produces a latex that is commonly used to treat neoplasms. This study aimed to evaluate the effects of diluted E. tirucalli latex (DETL) on human (SK-MEL-28) and canine (CBMY) melanoma cells. SK-MEL-28 (3 × 103 cells/well) and CBMY (6 × 103 cells/well) were cultivated in 96-well plates. The cells were treated with 50 μl/well of dilutions (1/2, 1/4, 1/8, 1/16, 1/32, 1/64, 1/128, 1/256, and 1/512) of a standard solution containing 1 mg/mL of the E. tirucalli latex (ETL) in DMEM. Control group cells received 50 μl/well of DMEM. After 24, 48, and 72 h of treatment, cell viability was assessed by the MTT assay. There was a significant decrease in viability at 48 and 72 hours after treatment for human melanoma cells and at 24, 48, and 72 hours for canine cells, mainly in higher dilutions of ETL. Human melanoma cells presented a typical U shape curve, characteristic of hormesis. To our knowledge, this is the first study showing inhibitory effects of DETL on canine melanoma cells. Therefore, DETL is a potentially new antineoplastic drug.
DEL-GRANDE, M. P. Photodynamic Therapy in the treatment of Ehrlich solid tumor in mice: efficacy evaluation and the systemic immune response. [Terapia Fotodinâmica no tratamento do tumor de Ehrlich inoculado em camundongos: Avaliação da eficácia e da resposta imunológica sistêmica]. 2013. 104 f. Dissertação (Mestrado em Ciências)
vidas de esforços, dedicação e trabalho me permitiram vivenciar oportunidades únicas, as quais palavras nunca serão o suficiente para agradecer. O seu exemplo de caráter, honestidade e companheirismo são fonte de inspiração para me tornar um ser humano melhor a cada dia. Agradeço a minha amada esposa Carolina de Lara Shecaira. Seu incrível companheirismo, suas palavras que me acalmam e seu esforço máximo em dar-me todo o apoio na confecção deste trabalho são evidências claras do quão afortunado sou por tê-la ao meu lado. Agradeço aos meus irmãos Heitor Penteado Del Grande e Mariana Penteado Del Grande Moré. A vida os escolheu como meus irmãos, eu os escolhi como amigos. Agradeço a toda a minha família, em especial as minhas avós Maria Jose Sanches e Pascoalina Del Grande e seus falecidos esposos, Francisco Penteado e Álvaro Del Grande.Agradeço a Kaya, minha querida amiga canina, falecida durante a confecção deste trabalho e que deixou muitas saudades. Obrigado por ter iluminado minha vida, Kaya.Agradeço do fundo de meu coração a minha queria orientadora Professora Maria Lucia Zaidan Dagli. Sem seus ensinamentos, sua dedicação e sua total confiança no meu potencial nada disso poderia ter sido possível. Muito obrigado por acreditar em mim.Agradeço ao Programa de pós-graduação Interunidades em Biotecnologia -USP -I. Butantan -IPT e a comissão de pós-graduação por todo apoio.
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