Inhibitory effects of Euphorbia tirucalli latex on murine B16/F10 melanoma cells and lung metastasis

Brunetti, Rafael Lanciani; Paz, Diego Pinha Alves da; Fonseca, Ivone Izabel Mackowiak da; Nagamine, Márcia Kazumi; Mori, Cláudia Madalena Cabrera; Grande, Murilo Penteado Del; Queiroz‐Hazarbassanov, Nicolle; Salles-Gomes, Cristina O. M.; Dagli, M.L.; Hernandez‐Blazquez, Francisco Javier

doi:10.3892/mco.2019.1916

Cited by 2 publications

(3 citation statements)

References 15 publications

(23 reference statements)

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“…Recently, our group reported that DETL not only reduced the cell viability in vitro but was also effective in inhibiting metastasis of murine B16/F10 melanoma cells [29].…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effects of Euphorbia tirucalli Lineu (Euphorbiaceae) Diluted Latex on Human and Canine Melanoma Cells

Paz

Nagamine

Grande

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Euphorbia tirucalli Lineu (Euphorbiaceae) is a tropical and subtropical ornamental and toxic plant. E. tirucalli produces a latex that is commonly used to treat neoplasms. This study aimed to evaluate the effects of diluted E. tirucalli latex (DETL) on human (SK-MEL-28) and canine (CBMY) melanoma cells. SK-MEL-28 (3 × 103 cells/well) and CBMY (6 × 103 cells/well) were cultivated in 96-well plates. The cells were treated with 50 μl/well of dilutions (1/2, 1/4, 1/8, 1/16, 1/32, 1/64, 1/128, 1/256, and 1/512) of a standard solution containing 1 mg/mL of the E. tirucalli latex (ETL) in DMEM. Control group cells received 50 μl/well of DMEM. After 24, 48, and 72 h of treatment, cell viability was assessed by the MTT assay. There was a significant decrease in viability at 48 and 72 hours after treatment for human melanoma cells and at 24, 48, and 72 hours for canine cells, mainly in higher dilutions of ETL. Human melanoma cells presented a typical U shape curve, characteristic of hormesis. To our knowledge, this is the first study showing inhibitory effects of DETL on canine melanoma cells. Therefore, DETL is a potentially new antineoplastic drug.

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“…Recently, our group reported that DETL not only reduced the cell viability in vitro but was also effective in inhibiting metastasis of murine B16/F10 melanoma cells [29].…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effects of Euphorbia tirucalli Lineu (Euphorbiaceae) Diluted Latex on Human and Canine Melanoma Cells

Paz

Nagamine

Grande

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…The U-shaped curve of cell viability, observed mainly in-human melanoma cells, suggests the hormesis effect in which a dose response of a cell or organism to a substance is biphasic. This effect means that cells benefit from low-dose stimulation by activation of the cellular stress response, outweighing the toxicity and suffer from a toxic or inhibitory effect when a high-dose of the agent is present ( Mattson and Cheng, 2006 ; Khaleghian et al., 2010 ; Archanjo et al., 2016 ; Altamimi et al., 2019 ; Brunetti et al., 2019 ). Further in vivo investigations of E. tirucalli latex had variable outcomes.…”

Section: Anticancer Properties Of Latex-bearing Plant Extractsmentioning

confidence: 99%

“…In the melanoma cancer model, induced using B16F10 cells inoculated into the tail vein of C57BL/6 mice the results were promising. Merely a 14-day treatment with a latex dilution was able to significantly reduce the volume of nodules in mice lungs resulting in only few melanoma colonies and no signs of congestion and hemorrhage within the lungs as well as no toxic alterations in kidneys, spleen or liver ( Brunetti et al., 2019 ). Other studies had presented E. tirucalli aqueous latex solution to act pro-angiogenic through increase in neoangiogenesis and promotion of formation of vascular networks in chorioallantoic membranes (CAMs) of fertilized chicken eggs ( Bessa et al., 2015 ).…”

Section: Anticancer Properties Of Latex-bearing Plant Extractsmentioning

confidence: 99%

Tap the sap – investigation of latex-bearing plants in the search of potential anticancer biopharmaceuticals

Mazur

Bałdysz²,

Warowicka³

et al. 2022

Front. Plant Sci.

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Latex-bearing plants have been in the research spotlight for the past couple of decades. Since ancient times their extracts have been used in folk medicine to treat various illnesses. Currently they serve as promising candidates for cancer treatment. Up to date there have been several in vitro and in vivo studies related to the topic of cytotoxicity and anticancer activity of extracts from latex-bearing plants towards various cell types. The number of clinical studies still remains scarce, however, over the years the number is systematically increasing. To the best of our knowledge, the scientific community is still lacking in a recent review summarizing the research on the topic of cytotoxicity and anticancer activity of latex-bearing plant extracts. Therefore, the aim of this paper is to review the current knowledge on in vitro and in vivo studies, which focus on the cytotoxicity and anticancer activities of latex-bearing plants. The vast majority of the studies are in vitro, however, the interest in this topic has resulted in the substantial growth of the number of in vivo studies, leading to a promising number of plant species whose latex can potentially be tested in clinical trials. The paper is divided into sections, each of them focuses on specific latex-bearing plant family representatives and their potential anticancer activity, which in some instances is comparable to that induced by commonly used therapeutics currently available on the market. The cytotoxic effect of the plant’s crude latex, its fractions or isolated compounds, is analyzed, along with a study of cell apoptosis, chromatin condensation, DNA damage, changes in gene regulation and morphology changes, which can be observed in cell post plant extract addition. The in vivo studies go beyond the molecular level by showing significant reduction of the tumor growth and volume in animal models. Additionally, we present data regarding plant-mediated biosynthesis of nanoparticles, which is regarded as a new branch in plant latex research. It is solely based on the green-synthesis approach, which presents an interesting alternative to chemical-based nanoparticle synthesis. We have analyzed the cytotoxic effect of these particles on cells. Data regarding the cytotoxicity of such particles raises their potential to be involved in the design of novel cancer therapies, which further underlines the significance of latex-bearing plants in biotechnology. Throughout the course of this review, we concluded that plant latex is a rich source of many compounds, which can be further investigated and applied in the design of anticancer pharmaceuticals. The molecules, to which this cytotoxic effect can be attributed, include alkaloids, flavonoids, tannins, terpenoids, proteases, nucleases and many novel compounds, which still remain to be characterized. They have been studied extensively in both in vitro and in vivo studies, which provide an excellent starting point for their rapid transfer to clinical studies in the near future. The comprehensive study of molecules from latex-bearing plants can result in finding a promising alternative to several pharmaceuticals on the market and help unravel the molecular mode of action of latex-based preparations.

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Inhibitory effects of Euphorbia tirucalli latex on murine B16/F10 melanoma cells and lung metastasis

Cited by 2 publications

References 15 publications

Inhibitory Effects of Euphorbia tirucalli Lineu (Euphorbiaceae) Diluted Latex on Human and Canine Melanoma Cells

Inhibitory Effects of Euphorbia tirucalli Lineu (Euphorbiaceae) Diluted Latex on Human and Canine Melanoma Cells

Tap the sap – investigation of latex-bearing plants in the search of potential anticancer biopharmaceuticals

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