The antioxidant potential of various extracts was evaluated using different antioxidant assays such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, ferric reducing antioxidant power (FRAP) assay, and 2,2-azinobis-ethylbenzothiozoline-6-sulphonic acid (ABTS) using UV spectrophotometer. The highest absorbance was observed in ethanolic extracts (EEs) of Euphrasia stricta 71.92 ± 1.22%, 65.77 ± 1.38%, and 67.88 ± 0.74%, followed by methanolic extracts (MEs) 70.14 ± 0.82%, 64.84 ± 0.74%, and 65.48 ± 1.40% for DPPH assay (517 nm), FRAP assay (700 nm), and ABTS assay (734 nm), respectively. The EEs of Euphorbia platyphyllos L. showed the antioxidant activity of 69.76 ± 1.48%, 64.42 ± 0.88%, and 65.54 ± 1.36% and MEs 68.00 ± 1.50%, 62.92 ± 0.64%, and 63.42 ± 0.94% for DPPH, FRAP, and ABTS assays, respectively. So, this research suggested that these medicinal plants possess a significant antioxidant potential and are important source of natural antioxidants and can be effectively used in treating oxidative stress disorders.
Medicinal plants are used as an important source of medicines in pharmaceutical industry. Rubia cordifolia is widely used to cure diabetes mellitus. Present study was aimed to investigate the antihyperglycemic effects of different fractions of R. cordifolia leaves and to analyze its antioxidant effect and phytochemical composition. Male albino mice were randomly distributed into seven groups (n = 7). Group-I was normal control, group-II was Alloxan (100 mg/kg)-induced diabetic control, and group-III was standard drug (Glibenclamide 0.5 mg/kg)-treated group. Animals in groups IV–VII were treated with n-hexane fraction, ethyl acetate fraction, n-butanol fraction and aqueous fraction of R. cordifolia, orally administered (100 mg/kg) once daily up to 28 days after Alloxan induction, respectively. Methanolic extract (ME) and fractions of R. cordifilia were analyzed for antioxidant activity and quantification of total phenolic content and total flavonoid content. HPLC of ME and most active fractions were performed. The results showed that RCEF (G-V) and RCBF (G-VI) have significantly (P < 0.05) reduced the increased level of glucose as compared to toxicant control group. It was further revealed that EF and BF have higher antioxidant activity (having IC50 34.9, 36.86 (µg/mL)) owing to phenolic and flavonoid identified by HPLC.
Current study was conducted to address the problem of quackery that is widespread in Dera Ismail Khan Pakistan. During this study number of quacks and Qualified professionals in these areas and different ways of malpractice, practiced by the quacks were included. The study was conducted in all the urban, rural and semi urban area of DIK. Survey based on questionnaire was conducted distributing one in general populations and 2nd distributed among the quacks in the rural, urban and semi urban areas of DIK. Sample size of 150 was set in this regard. The three months duration study was performed to assess their malpractice regarding quacks and what knowledge they have. A questionnaire to approach the behavior of quacks regarding their practice consisting of 11 questions was also designed to analyze ways of quackery, government role and awareness of people about the quackery. Quacks mostly cater to people who are lower middle and lower socioeconomic class, having no approach to qualified practitioners. The public is not quality conscious and they seek to relive the pains with restricted budgets. These quackers consider them a qualified practioners and people having less knowledge of its outcomes and possessing poverty, expose to poor health practices. This leads to spread of other fatal diseases like AIDS, hepatitis and cancer. In order to reduce probability of exposure to unsafe healthcare practices, Government must have proper check and balance so that it may be proactively addressed by shutting down all unlicensed practitioners and educating the community to discourage the malpractices in field of health.
Nystagmus is involuntary up and down, to and fro and oscillatory movement of eye. Nystagmus is caused by different inheritance pattern, which can be; AD (autosomal dominant), AR (autosomal recessive) and X-linked. This review elaborates the mutational canvas of FRMD7 gene involved in X-linked Nystagmus. In Pakistan the publication is either not available or no research findings has been reported for FRMD7 gene according to our knowledge.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.