Antihyperglycemic effect and phytochemical investigation of Rubia cordifolia (Indian Madder) leaves extract

Abstract

Medicinal plants are used as an important source of medicines in pharmaceutical industry. Rubia cordifolia is widely used to cure diabetes mellitus. Present study was aimed to investigate the antihyperglycemic effects of different fractions of R. cordifolia leaves and to analyze its antioxidant effect and phytochemical composition. Male albino mice were randomly distributed into seven groups (n = 7). Group-I was normal control, group-II was Alloxan (100 mg/kg)-induced diabetic control, and group-III was standard drug (Glibenclamide 0.5 mg/kg)-treated group. Animals in groups IV–VII were treated with n-hexane fraction, ethyl acetate fraction, n-butanol fraction and aqueous fraction of R. cordifolia, orally administered (100 mg/kg) once daily up to 28 days after Alloxan induction, respectively. Methanolic extract (ME) and fractions of R. cordifilia were analyzed for antioxidant activity and quantification of total phenolic content and total flavonoid content. HPLC of ME and most active fractions were performed. The results showed that RCEF (G-V) and RCBF (G-VI) have significantly (P < 0.05) reduced the increased level of glucose as compared to toxicant control group. It was further revealed that EF and BF have higher antioxidant activity (having IC50 34.9, 36.86 (µg/mL)) owing to phenolic and flavonoid identified by HPLC.

Overview of Effective Traditional Medicinal Plants having Antihyperlipidemic Activity

“…R. cordifolia's antidiabetic properties are linked to antioxidant activity and phytochemical components such as phenolics and flavonoids, particularly mangiferine and purpurine. Tannins, terpenoids, alkaloids, and saponins, among other phytochemicals, may have a synergistic effect 40 . Rubia cordifolia plays a vital role in treating atherosclerosis and other cardiovascular disorders which was confirmed based on their proven scientific evidence of antihyperlipidemic and antioxidant activities.…”

Role of Medicinal Plants in the Management of Diabetes Mellitus: A Review

“…It is herbaceous climbing plant with thin red bark, commonly known as majith, belonging to the family of Rubiaceae [74]. Further investigations showed that certain solvent fractions like ethyl acetate and and n-butanol fractions have significant antidiabetic effect [75]. Tripathi YB et al found Rubiadin, dihydroxy anthraquinone, is main active chemical constituent has the antioxidant activity.…”