Background Bone morphogenetic protein receptor 1B (BMPR1B) gene is one of candidate genes for reproductive and growth traits in sheep. The present study was aimed to detect the Booroola (FecB) allele in BMPR1B gene and its association with growth traits in MEGA (Merino × Garut) sheep. A total of 82DNA samples collected from individual lamb (mixed-sex) blood were genotyped for allelic polymorphism using a PCR–RFLP method. Results The PCR analysis in BMPR1B gene resulted the amplicons with size of140 bp. The RFLP analysis with AvaII restriction enzymeresultedtwo allelic types of wildtype (A/Fec+) and mutant or Booroola (G/FecB) with frequency of 0.89 and 0.11, respectively. However, the genetic diversity in BMPR1B/AvaII gene of animal studies was categorized tolow category (PIC = 0.18)and under in a genetic equilibrium (χ2 = 1.25). Conclusions Itshowed us that carrying FecB allele in the heterozygous sheep were not associated with growth traits in MEGA sheep.
Toba Lake is one of the largest caldera lakes in the world, formed by an enormous eruption about 74,000 years ago. The emergence of hot spring manifestations around Lake Toba is closely related to volcanic activity, which is also controlled by regional geological structures. There are two areas in Lake Toba: Simbolon and Pusuk Buhit, with several geothermal manifestations, such as fumaroles, hot springs, and steaming ground. With the large caldera area, there should be other locations with geothermal potential. Therefore, we analyzed the regional gravity anomaly to find specific patterns that might indicate a possible geothermal field. First, we obtained the data of Free Air Anomaly (FAA) with a resolution of 1.85 km from altimetry satellites and acceleration of gravity anomaly data of 0.1 mGal at 1 minute per grid with an altitude accuracy of 1 meter. In order to acquire the Simple Bouguer Anomaly, the data were corrected. Then, we analyzed the results based on derivative analysis. The result shows that the location has simple Bouguer anomaly values between - 26.2 mGal-36.0 mGal with an initial density of 1.74 gr/cc. Furthermore, we speculated that indicated regional patterns of structures and sub-surface properties might be associated with that prospective geothermal.
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