Authors' Contribution SMH supervised the project and provided the facilities for the research. MZHA collected the data and compiled and analyzed the results. AJ helped in statistical analysis. AIH analyzed the nutrients. NA did biochemical analysis. QA analyzed the data statistically and interpreted the results. MH collected the samples. MMHR prepared the manuscript. MMS helped in research work and collection of growth data. AK assisted in collection of fecal matter. DR conducted feeding trials.
Authors' Contribution MMS conducted the research and wrote the manuscript. SMH planned and supervised the research. FJ provided the research facilities. MIH helped in chemical analysis of minerals. AJ helped in statistical analysis and interpretation of the results. MA helped in manuscript preparation. MZHA helped in conducting feeding trial and feces collection.
Phytic acid’s presence in low-cost Moringa by-products effect the availability of important nutrients, diminishing the fish quality and blood composition in fish. Phytate having chelating effects with nutrients and minerals, can be reduced by the supplementation of phytase enzyme. Without the use of enzyme, plant meal may cause water pollution and decrease the fish health that results in higher culture cost. Therefore, current study was designed to check improvement in overall performance of
Catla catla
fingerlings fed Moringa by product-based diets supplemented with phytase (0, 300, 600, 900, 1200 and 1500, FTU/kg). All diets were integrated with non-digestible marker (Cr
2
O
3
) at the rate of 1%. The fingerlings were fed couple of times a day (4% of live wet weight). Results showed significant (
p < 0.05
) improvement in nutrient digestibility (i.e. EE, CP and GE), carcass composition and hematological parameters (i.e. RBCs, PLT and Hb) at 900, FTU/kg of phytase in contrast with other treatments. Moreover, phytase addition improves the water quality by reducing the nutrients leaching through feces at low cost. Current results indicated that, using mixture of Moringa seed meal and Moringa leaf meal based diet supplemented with phytase at 900, FTU/kg concentration is the most optimum level to develop a cost-effective as well as eco-friendly fish feed with maximum absorption of important nutrients and minerals in fish body resultantly high higher fish performance.
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