SUMMARY:The current study aimed to provide the topography and renal biopsy of the standing mare kidneys by laparoscopy w ithout CO 2 insufflation and to compare between the use of biopsy needle and forceps. Five clinically healthy adult nonpregnant mares weighing 250-300 Kg and aging 7-9 years were used in the current work. The gasless laparoscopic renal biopsy appeared simple, safe, reliable, minimal invasive, timesaving and economical technique. The parallel biopsy portals provided easy and accessible biopsy procedure than dorsal or ventral portals. The biopsies taken from the lateral surface were less hemorrhagic than those taken from the caudal pole. This study recommended the laparoscopic forceps because the biopsy forceps provided satisfactory and representative specimens with minimal hemorrhage than Tru-cut needle.
Natural resources are rich in collagen and growth factors that can perfectly fit in tissue engineering and contribute to the extracellular matrix of many tissues. The thin membrane that coats the inner surface of the eggshell is one of these resources that meets the need of tissue engineering as it contains high collagen, growth factors, and glycosaminoglycans. With advanced technology, eggshell membrane (EgS-M) has been available in many forms like powder, solution, and thin sheets for broad applications. Furthermore, integrating nanoparticles has provided a broad-spectrum EgS-M biomaterial with antimicrobial, anti-inflammatory, biocompatible, and biodegradable properties. Herein, we discuss the current status of EgS-M's contribution to tissue engineering of skin, cartilage, bone, periodontal, myocardium, and nerve.
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