Posterior capsule opacification (PCO) is one of the most common complications of phacoemulsification with intraocular lens (IOL) implantation. This retrospective study evaluated the incidence of neodymium: YAG (Nd:YAG) laser capsulotomies for PCO in two groups of patients with different sizes of poly(methyl methacrylate) (PMMA), capsular bag-fixated IOLs. We evaluated 437 eyes that had phacoemulsification with continuous curvilinear capsulorhexis over a four-year period. Patients were placed in one of two groups, based on IOL size: large IOL (lens length 13.5 mm or greater) and small IOL (lens length less than 13.5 mm). In the large IOL group, 106 of 280 patients (38%) required YAG laser posterior capsulotomy; in the small IOL group, 25 of 157 patients (16%) did. This difference was statistically significant even though the average follow-up was 84.1 weeks in the large IOL group and 54.1 weeks in the small IOL group.
Membrane vesicles from the envelope of Escherichia coli were separated by electrophoresis through dilute agarose and by sizing chromatography through Sephacryl S-1000. These techniques revealed that proteins were associated with different subsets of vesicles. In particular, dilute agarose electrophoresis clearly separated the inner membrane penicillin-binding proteins (PBPs) into different vesicle groups. Vesicles containing PBPs 4, 6, 7, and 8 migrated rapidly through agarose; vesicles with PBPs 1a, 1b, 2, 3, and 5 eluted later. With the exception of PBP 4, which migrated with PBPs 1 through 5, chromatography through Sephacryl S-1000 was able to distinguish the same two vesicle sets, though the extent of separation was poorer than with agarose. The existence of these associations among vesicles and proteins suggests that there is an organization to the inner membrane of E. coli which is not observed when membrane vesicles are separated solely on the basis of density in sucrose gradients.
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