We compared two clinical video-assisted specular microscopes (Zeiss, noncontact, and the wide-field Keeler Konan sp 3300, contact) with an autofocus microscope (Konan noncon Robo-ca sp 8000, noncontact) with built-in analyzing software by studying the morphometry of the central corneal endothelium of 12 eyes in 12 patients. The mean coefficients of variation of the cell size analysis of three successive images per cornea for the three methods were 3.9%, 4.0%, and 2.2%, respectively. One-way analysis of variance showed a significant difference in the mean cell area measured with the three methods; there was no significant difference in the results of hexagonality (pleomorphism) and coefficient of variation (polymegathism). Although each microscope showed a high reproducibility, we do not recommend using the three interchangeably. The Konan noncon Robo-ca sp 8000 specular microscope provides a rapid morphometric endothelial analysis. The Keeler Konan sp 3300 is better for more detailed studies and photography of the corneal endothelium.
The central corneal endothelium of 13 eyes in 13 subjects was visualized with a non-contact specular microscope. This report describes the computer-assisted morphometric analysis of enhanced digitized images, using a direct input by means of a frame grabber. The output consisted of mean cell area, cell density, frequency distribution of the individual cell area, and cell polygonality. Results showed that the mean coefficient of variation of images analyzed three times consecutively was 0.95 percent. The cell analysis of three different images of the same subject was accurate, with a mean coefficient of variation of 4.2 percent.
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