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A total of 100 different E. coli isolates collected from 10 different biological and environmental sources (10 isolates from each source) such as human faces, human urine, cattle, sheep, goat, chicken, duck, pigeon, drain sewage and soil were used for in-vitro drug sensitivity test in the

To examine the groundwater quality a total of 120 groundwater samples were collected from two southern districts; Barguna (27 points) and Patuakali (33 points) of Bangladesh in two seasons viz., pre-monsoon (60 samples) and post-monsoon (60 samples) in 2012. A comprehensive analysis has been conducted to explore the groundwater hydrochemistry and effects of major ions on the suitability for potable water and agricultural uses. Geochemical analyses have clearly shown that the seasonal effect does change the order of abundance of major ions in the groundwater, while principal component analysis confirmed the reasons of extreme salinity and hardness. Piper diagram showed that the predominant hydrochemical facies for the groundwater were Na ? -Cl --SO 4 2-and Na ? -K ? -HCO 3 -types. The chloroalkaline index confirmed that the ion exchange was governed mainly in water phase. Equiline diagram explained the relationship among the alkali with total cations and anions where cations were more dominant than anions at 1:1 ratio. The Gibb's diagram illustrated the chemical composition of water mainly influenced by precipitation from the water sources. The molar ratio Cl -/Ranions and Na ? /Na ? ?Cl -indicated that the groundwater of the study area was affected by seawater intrusion. In addition to unsuitable salinity ([5000 ls/cm), Kelly's ratio, total hardness, sodium absorption ratio, residual Sodium carbonate, and Na % clearly indicated that the groundwater in the study area were not suitable for drinking, irrigation, and domestic uses. Moreover, the spatial distribution maps were drawn to observe the spatial extent of EC, TDS, SAR, and SSP using IDW technique. Thus, considering all the above findings this area needs special attention to ensure the supply of potable water and to maintain sustainable agricultural activities.

The study was undertaken with a view to compare clinical and laboratory diagnoses of various bacterial diseases of poultry during the period from March 2009 to February 2010 in the laboratory of the Dept. of Microbiology and Hygiene, Bangladesh Agricultural University (BAU), Mymensingh. A total of 135 sick and dead chickens (47 broilers and 88 layers) were collected from 12 different poultry farms (4 broilers and 8 layers) of Mymensingh and Gazipur districts which were subjected for clinical followed by laboratory diagnosis. Clinical diagnosis was made on the basis of clinical history, clinical signs and postmortem findings of the affected birds whereas; confirmatory diagnosis was made by using cultural examination, Gram's staining and different biochemical tests. In this study, out of 47 broilers, 16 (34%) chickens were clinically diagnosed as colibacillosis, 11 (23.40%) as salmonellosis and 2 (4.25%) as fowl cholera. In the same way, out of 88 layer chickens, 28 (31.82%) were diagnosed as colibacillosis, 16 (18.18%) as salmonellosis and 11 (12.50%) as fowl cholera. In laboratory, out of 47 suspected broiler chickens, 12 (25.53%) chickens were diagnosed as colibacillosis, 7 (14.89%) as salmonellosis and 0 (0%) as fowl cholera. Correspondingly of the 88 layer chickens 22 (25%) were diagnosed as colibacillosis, 11 (13.64%) as salmonellosis and 8 (9.09%) as fowl cholera. So the findings concluded that clinical diagnosis is not always accurate like laboratory diagnosis because in most cases clinical history, clinical signs and post-mortem lesions of different bacterial diseases including mixed infections are almost similar to other related diseases and it is recommended to confirm laboratory diagnosis before treatment of the diseases.

The present research work was undertaken for the characterization of the bacterial pathogens responsible for pneumonia in black Bengal goats. Nasal swab samples (n = 50) were collected from the pneumonic black Bengal goats in Mymensingh and Sirajgonj districts. Samples were inoculated onto nutrient agar, eosin methylene blue (EMB) agar, MacConkey agar, and blood agar media for isolation of bacteria. Identification of bacteria was performed by the Gram's staining method, cultural properties and biochemical tests. Antibiotic sensitivity of bacterial isolates was performed against 11 antimicrobial agents. Pasteurella spp were isolated from 25 cases, and Staphylococcus spp from 13 cases. Mixed infection caused by the Pasteurella spp and Staphylococcus spp. were recorded in 12 cases. Pasteurella spp produced whitish, opaque circular and translucent colonies on nutrient agar, smooth, convex, glistening colonies on EMB agar and no hemolysis on blood agar. Staphylococcus spp have shown gray white or golden yellowish colonies on  nutrient agar. Golden yellow colonies without hemolysis or whitish colonies with hemolysis were also produced by Staphylococcus spp. on the blood agar media. Pasteurella spp were indole positive, MR-VP negative and ferment dextrose, sucrose and mannitol with the production of acid. The Staphylococcus spp were positive to MR-VP, coagulase and catalase reactions, negative to indole test and fermented five basic sugars with acid production. Results of cultural and biochemical tests supported that these two isolates belonged to P. multocida and S. aureus. P. multocida were highly sensitive to ciprofloxacin and resistant to penicillin. S. aureus found to be highly sensitive to erythromycin, tetracycline, enrofloxacin, and norfloxacin and less sensitive to amoxicillin. DOI = http://dx.doi.org/10.3329/bjvm.v9i1.11215Bangl. J. Vet. Med. (2011). 9(1): 67-71

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The present study attempted to determine the economic returns of alternative farming system of integrated farming aiming to address the livelihood security of small farm households. One hundred and sixty small and medium farmers practicing in four different FS such as Crop-Poultry (C-P), Crop-Poultry-Pond fisheries (C-P-F), Crop-Livestock-Poultry (C-L-P) and Crop-Livestock-Poultry-Pond fisheries (C-L-P-F) were selected from Mymensingh, Jamalpur and Sherpur district. The main information collected were socio-demographic characteristics of farm households, household income from farm and non-farm sources, size of land holding and farming system followed, livestock and poultry rearing and pond fish culture, and effect of integrated farming on household income and livelihood security of small farm households. Data were collected covering one production period during 2009-2010. For estimating farm income of small farm household, whole farm approach was considered. Increased number of farm enterprises caused higher farm income. However, considering the small farm practices and whole farm approach, the farm income as well as net return was higher for C-L-P-F farmers (Tk. 57002), followed by C-L-P (Tk. 52978), C-P-F (Tk. 20447) and C-P (Tk. 13734) farmers. Some of the most commonly used factors in the assessment of livelihood security included those related to household income and expenditure, expenditure spent on food, adequacy of food taken, household structure and facilities, owning household assets, and access to drinking water and sanitation, and other basic needs of farm household. All these socioeconomic factors were found to be related livelihood security of small farmers in Bangladesh. Women were more involved in income earning activities and household decision making with the introduction of integrated farming in small farm condition.DOI: http://dx.doi.org/10.3329/agric.v10i1.11065The Agriculturists 2012; 10(1): 51-63

The decomposition rates of organic nitrogen and organic phosphorus in lakes and rivers can be important parameters in water quality model calibrations. Commonly, decomposition rate coefficients are calibrated by observing the changes in each component in the field survey. However, in natural field conditions, production and decomposition occur at the same time, and the calibrations may result in uncertainties. In this study, water samples collected from six major rivers in Korea were incubated for 20 days in a dark incubator and the decomposition rates of POP, DOP, LDOP, PON, DON, LPON, LDON, ammonia, and nitrite were determined. By excluding the interference of photosynthesis, animal grazing, and excretion, the decomposition rate coefficients were determined with less uncertainty than in natural conditions. The decomposition data were fit to first-order models of several decomposition pathways, including POP ! DIP,The decomposition rates of dissolved forms were five-seven times higher than particulate forms. And the rates of organic P (0.149 day �1 for LPOP and 0.718 day �1 for LDOP) were 1.6 times higher than those of organic N (0.093 day �1 for LPON and 0.472 day �1 for LDON). Traditionally, the decomposition rates of organic phosphorus and organic nitrogen are not discriminated; the same rate is applied to organic carbon, nitrogen, and phosphorus. The result of this study shows that organic phosphorus and nitrogen should be given different decomposition rates for more accurate models. The rate coefficients measured in this study will provide a guide to the selection of model parameters for water quality modeling.