Clinical and Laboratory Diagnoses of Newcastle and Infectious Bursal Diseases of Chickens

Hasan, A.; Ali, M.Y.; Siddique, Mahbubul Pratik; Rahman, Muhammad Mahbubur; Islam, Mohammad Jahidul

doi:10.3329/bjvm.v8i2.11196

Cited by 20 publications

(31 citation statements)

References 26 publications

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“…Our study (95) observes that, there might be the possible difference among the bursal samples for pathogenesis of IBD were irreversible bursal follicle damage and IgM-bearing B lymphocytes and others cell damage (Rodriguez-Chavez et al, 2002), those were more severe in haemorrhagic bursa compared to oedematous and atrophied bursa which increased the sensitivity in haemorrhagic bursa compared to oedematous and atrophied bursa for IBDV. RT-PCR is used to detect viral RNA in homogenates of infected organs or embryos without considering the viability of the virus present (Hasan et al, 2010). RT-PCR works on hypervariable region (VP2).…”

Section: Resultsmentioning

confidence: 99%

“…RT-PCR performed on a MJ Mini Thermocycler, Bio-Rad, USA. Primers Vv-fp775 (forward primer, 5′-AATTCTCATCACAGTACCAAG-3′) and Vv-rp1028 (reverse primer, 5′-GCTGGTTGGAATCACAAT-3′) were used to amplify a 253 bp fragment of the VP2 gene (Hasan et al, 2010). RT-PCR was performed at 42°C for 1 h followed by activation of Taq polymerase at 94°C for 2 min, then 35 cycles of denaturation at 94°C for 30 s, annealing at 45°C for 1 min and elongation at 60°C for 1.5 min.…”

Section: Rt-pcrmentioning

confidence: 99%

“…The molecular methods like RT-PCR is used to amplify the reverse transcription of the DNA code (Saiki et al, 1985). RT-PCR is the method which helps for precise and early diagnosis of viruses from field samples (Hasan et al, 2010). Among these diagnostic methods, in this study we chose ODIT and RT-PCR as ODIT is simplest of all, inexpensive and has the ability for detecting specific antibodies (Dey et al, 2019;Hornbeck, 2017;Ouchterlony, 1948;Ouchterlony and Nilsson, 1986) and RT-PCR is specific, sensitive, speedy, reliable and accurate techniques for detection of IBD viruses (Hasan et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Fresh samples from the affected chickens are usually more suitable to detect the virus but in rural areas of Bangladesh, there is very limited opportunity for the identification of poultry disease at the field level. The diagnosis is done mainly based on the clinical history, sign-symptoms and necropsy findings where there is a high possibility of misinterpretation and wrong diagnosis, because many poultry diseases produce similar clinical signs, symptoms and postmortem findings which are sometimes very difficult to differentiate (Hasan et al, 2010;Sali, 2019). Therefore, this study was designed to compare the sensitivity of the molecular technique (RT-PCR) and serological technique (ODIT) for the detection of IBDV from three types of bursal samples.…”

Section: Introductionmentioning

confidence: 99%

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Efficacy of two diagnostic tests for the detection of infectious bursal disease viruses in chicken from different types of bursal samples

Afrin¹,

Chowdhury²,

Cho³

et al. 2020

Afr. J. Microbiol. Res.

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Infectious Bursal Disease (IBD) is an economically important irreversible immunosuppressive disease of young birds. The present study was designed to confirm the efficacy of two common diagnostic tests for the detection of Infectious Bursal Disease Virus (IBDV) from the three types of bursal samples collected from a recent outbreak in layer and broiler chickens of Gazipur district, Bangladesh. This study compared the degree of sensitivity between Ouchterlony Double Immunodiffusion Test (ODIT)and Reverse Transcription Polymerase Chain Reaction (RT-PCR) for the detection of IBD viral antigen from the bursal samples. A total of 180 field bursal samples (80 broiler and 100 from layer chicken) from suspected IBDV infected dead chickens were collected from 50 different poultry farms. Bursal homogenates were used to detect IBDV using ODIT and RT-PCR. Three types of bursal samples, hemorrhagic bursa (90), edematous bursa (78) and atrophied bursa (12) were selected for the detection of viral antigen. A panel of anti-sera and IBDV specific primer for VP2 gene was used for RT-PCR. The data demonstrated that, out of 180 field samples, 164 (91%) were positive by RT-PCR and 120 (67%) were positive by ODIT. Haemorrhagic bursas were more sensitive compared to oedematous bursas while no virus was detected from the samples of atrophied bursa. This study demonstrated that, RT-PCR was more sensitive and effective diagnostic tool compared to that of ODIT.

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Section: Resultsmentioning

confidence: 99%

Section: Rt-pcrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Efficacy of two diagnostic tests for the detection of infectious bursal disease viruses in chicken from different types of bursal samples

Afrin¹,

Chowdhury²,

Cho³

et al. 2020

Afr. J. Microbiol. Res.

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“…The disease is characterized by neurological symptoms (e.g. tremors, tonic/clonic spasms, wing/leg paresis or paralysis, torticollis, and aberrant circling behaviour), weak limbs, cyanosis of the wattle and comb, nasal and eye discharges, greenish diarrhoea, weight loss, loss of egg production and high mortalities (Cattoli et al, 2010;Rakibul Hasan et al, 2010;OIE, 2012). At post-mortem, the characteristic lesions may include haemorrhages in the trachea, brain and spleen.…”

Section: Introductionmentioning

confidence: 99%

A retrospective study and predictive modelling of Newcastle Disease trends among rural poultry of eastern Zambia

Mubamba

Ramsay

Abolnik

et al. 2016

Preventive Veterinary Medicine

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Newcastle disease (ND) is a highly infectious disease of poultry that seriously impacts on food security and livelihoods of livestock farmers and communities in tropical regions of the world. ND is a constant problem in the eastern province of Zambia which has more than 740 000 rural poultry.Very few studies give a situational analysis of the disease that can be used for disease control planning in the region. With this background in mind, a retrospective epidemiological study was conducted using Newcastle Disease data submitted to the eastern province headquarters for the period from 1989 to 2014. The study found that Newcastle Disease cases in eastern Zambia followed a seasonal and cyclic pattern with peaks in the hot dry season (Overall Seasonal Index 1.1) as well as cycles every three years with an estimated provincial incidence range of 0.16 to 1.7% per year.Annual trends were compared with major intervention policies implemented by the Zambian government, which often received donor support from the international community during the study period. Aid delivered through government programmes appeared to have no major impact on ND * Dr Chrisborn Mubamba BVM, MTrVSc, PhD candidate, James Cook University, Queensland, Australia. 1 Solander Drive, QLD 4812, Phone-+610450009774, EmailChrisborn.mubamba@my.jcu.edu.au or chrisbornmw@yahoo.com 1 trends between 1989 and 2014 and reasons for this are discussed. There were apparent spatial shifts in districts with outbreaks over time which could be as a result of veterinary interventions chasing outbreaks rather than implementing uniform control. Data was also fitted to a predictive time series model for ND which could be used to plan for future ND control. Time series modelling showed an increasing trend in ND annual incidence over 25 years if existing interventions continue. A different approach to controlling the disease is needed if this trend is to be halted. Conversely, the positive trend may be a function of improved reporting by farmers as a result of more awareness of the disease.

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Determinants of Newcastle disease in commercial layer chicken farms in two districts of Bangladesh: A case-control study

Roky

Das

Akter

et al. 2022

Heliyon

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Clinical and Laboratory Diagnoses of Newcastle and Infectious Bursal Diseases of Chickens

Cited by 20 publications

References 26 publications

Efficacy of two diagnostic tests for the detection of infectious bursal disease viruses in chicken from different types of bursal samples

Efficacy of two diagnostic tests for the detection of infectious bursal disease viruses in chicken from different types of bursal samples

A retrospective study and predictive modelling of Newcastle Disease trends among rural poultry of eastern Zambia

Determinants of Newcastle disease in commercial layer chicken farms in two districts of Bangladesh: A case-control study

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