Background: Tooth extraction is the process of removing a tooth from the oral cavity potentially triggering a wound healing response in the body. As a result, many methods have been applied to improve the wound healing process, especially in wounds resulting in complications. One such method involves the application of amniotic membrane which has anti-inflammatory, anti-bacterial, anti-fibrosis, anti-scarring properties with low immunogenicity, epithelialization effects, and secretory leukocyte protease inhibitor (SLPI). It also contains collagen, various growth factors, transferrin, fibronectin, nidogen, proteoglycans, hyaluronan, and laminin. Purpose: This study aimed to determine the effects of sponge amnion on the number of VEGF expressions and new blood vessels in post-tooth extraction wounds of Wistar rats. Methods: Sponge amnion was produced by mixing freeze-dried amnion membrane from the Tissue Bank at RSUD Dr. Soetomo with 1% gelatin before freeze drying the mixture. Wistar rats were then divided into two groups. In Group 1, referred to as the control group, the post-extraction wounds of the rats received no treatment. Meanwhile, in Group 2, the treatment group, the subjects’ post-extraction wounds were treated with sponge amnion. The rats of both groups were sacrificed on day 3 to allow observation of the number of VEGF expressions and new blood vessels. A statistical analysis test, a t-test, was subsequently conducted. Results: There was a significant difference in the number of new blood vessels in the control group and that of the treatment group with a p value of 0.018 (p<0.05). There was also a significant difference in VEGF expression between the two groups with a p value of 0.000 (p <0.05). Conclusion: Sponge amnion can generate a number of VEGF expressions and new blood vessels in the post-extraction wounds of Wistar rats.
Background: Calcium is a material that is mostly contained in the Anadara-granose shell. Beta-TCP can be obtained from the hydrothermal process from the Anadara-granose shell. Beta-TCP has a chemical composition that approximates the structure of bones and teeth. Objective:The microencapsulation technique aims to increase stability, reduce side effects and toxic effects of drugs, and prolong the release of ingredients. The encapsulation process is an attempt to inhibit the dissolution speed of Calcium to prevent tunnel defects. Methods: Anadara-granose shell powder was subjected to hydrothermal processing for 18 hours and sintering for 3 hours. The beta-TCP powder was dissolved with aquadest using a magnetic stirrer until it was homogeneous, Na-alginate was dissolved in aquadest until it was homogeneous with a magnetic stirrer then the two solutions were mixed and the CaCl2 solution was dropped. The sample was divided into 3 groups; Pure Beta-TCP(K-); 7 hours stirring (P1); 8 hours stirring (P2). After completion of the stirrer, the samples were centrifuged at 2500 rpm for 6 minutes, then freeze-dried for 12 hours. The level test was carried out using complexometry comparing the pure Beta-TCP group with the Beta-TCP stirrer encapsulation process for 7 hours and 8 hours. Results: The data showed that the average calcium level in K(-) group with pure Beta-TCP was 8.63%, the P1 Beta-TCP group with 7 hours stirrer 2.86%, and the P2 Beta-TCP group with 8 hours stirrer 2.12%. Conclusion: In the Anadara-granosa shell nanoencapsulation process, the calcium level gradually decreased with the longer duration of stirring time
Introduction: Cross-infection is the transfer of microorganisms that can occur from individual to individual or to object. Candida albicans is one of the infectious pathogens that can cause cross-infection in dentistry. One example is the manufacture of working models of gypsum material, which can cause cross-infection from patients to dentists or technicians, so it is necessary to control infection by means of disinfection of type III gypsum material work models. Objective: To determine the effectiveness of soaking type III Gypsum in a 0.5% Sodium hypochlorite and 2% Povidone Iodine solution against the growth of Candida albicans. Methods: A laboratory experiment with a total of 21 samples, consisting of 7 samples for each group. The sample is made of a type III gypsum plate measuring 2 x 1 x 0.5 cm. The sample consisted of 3 groups: treatment 1, treatment 2, and treatment 3. The study used the diffusion method. Gypsum plate type III was contaminated with Candida albicans and then immersed in a solution of sterile distilled water (C), 0.5% sodium hypochlorite (P1), and 2% povidone-iodine (P2). The soaking results were planted in SDA media, incubated for 2x24 hours, and then counted using a colony counter. The data were analyzed using Kruskal- Wallis and Mann-Whitney post hoc analyses. Results: In the Kruskal- Wallis analysis of significance (p), between each group, there was a significant difference (p<0.05). The Mann-Whitney post hoc analysis test showed a correlation between group K and P1 and P2 (p<0.05). Conclusion: 0.5% Sodium Hypochlorite and 2% Povidone Iodine solutions are effective as disinfectants using the immersion method in Type III Gypsum against the growth of Candida albicans.
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