As a search for natural antioxidants from plant materials, strong antioxidative activity was observed in leaf waxes extracted from Eucalyptus species. A novel type of antioxidant was isolated from the leaf wax of Eucalyptus globulus and identified as «-tritriacontan-16,18-dione. Antioxidative activities were determined by different methods ; a thiocyanate method, a thiobarbituric acid method, a total carbonyl value method and a weighing test. The antioxidant showedremarkable antioxidative activity in a water/alcohol system and was more effective than a-tocopherol and BHA;however, it has no antioxidative activity in an oil system.
A lignan compound, P3, having strong antioxidative activity was found to be formed in high concentration during the industrial bleaching process of unroasted sesame seed oil. P3 (named sesaminol) was identical to a minor constituent previously isolated from acetone extract of sesame seed. It was shown that sesamolin in unprocessed sesame oil is the source of seaseminol, and the formation of seasaminol was confirmed by the model experiment with corn oil to which sesamolin had been added. Sesaminol was not so greatly removed by the deodorization process that follows bleaching as was sesamol, and it was shown to be at a concentration of ca. 100 mg/100g in commercial refined unroasted seed oil. The antioxidative activity of sesaminol was foughly equal to those of sesamol and γ‐tocopherol by the thiocyanate method. Therefore, it seems that the antionxidative activity of refined unroasted seed oil is mainly attributed to sesaminol.
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