BackgroundClock genes regulate circadian rhythm and are involved in various
physiological processes, including digestion. We therefore investigated the
association between the CLOCK 3111T/C single nucleotide
polymorphism and the Period3 (PER3)
variable-number tandem-repeat polymorphism (either 4 or 5 repeats 54 nt in
length) with morning gastric motility.MethodsLifestyle questionnaires and anthropometric measurements were performed with
173 female volunteers (mean age, 19.4 years). Gastric motility, evaluated by
electrogastrography (EGG), blood pressure, and heart rate levels were
measured at 8:30 a.m. after an overnight fast. For gastric motility, the
spectral powers (% normal power) and dominant frequency (DF, peak of the
power spectrum) of the EGG were evaluated. The CLOCK and
PER3 polymorphisms were determined by polymerase chain
reaction (PCR) restriction fragment length polymorphism analysis.ResultsSubjects with the CLOCK C allele (T/C or C/C genotypes: n =
59) showed a significantly lower DF (mean, 2.56 cpm) than those with the T/T
genotype (n = 114, 2.81 cpm, P < 0.05). Subjects
with the longer PER3 allele
(PER3
4/5 or
PER3
5/5 genotypes: n = 65) also showed a
significantly lower DF (2.55 cpm) than those with the shorter
PER3
4/4 genotype (n = 108, 2.83 cpm,
P < 0.05). Furthermore, subjects with both the
T/C or C/C and PER3
4/5 or
PER3
5/5 genotypes showed a significantly
lower DF (2.43 cpm, P < 0.05) than subjects with
other combinations of the alleles (T/T and
PER3
4/4 genotype, T/C or C/C and
PER3
4/4 genotypes, and T/T and
PER3
4/5 or
PER3
5/5 genotypes).ConclusionsThese results suggest that minor polymorphisms of the circadian rhythm genes
CLOCK and PER3 may be associated with
poor morning gastric motility, and may have a combinatorial effect. The
present findings may offer a new viewpoint on the role of circadian rhythm
genes on the peripheral circadian systems, including the time-keeping
function of the gut.
The 3111T/C single nucleotide polymorphism (SNP) of Circadian Locomotor Output Cycles Kaput (CLOCK) gene reportedly affects gastric motility before breakfast. It is of interest to know whether this SNP can affect the motility during the daytime. We investigated the association between the CLOCK 3111T/C SNP and several gastric motility parameters during the time period from 8:00 to 20:00 in 34 young women with scheduled meals. There were similar daytime fluctuations in gastric motility before and after the meals between the major (T/T) and minor (T/C) allele carriers. The CLOCK SNP may affect daytime gastric motility less than food stimulation.
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