The ability of two freshly isolated Boletus stains to fruit under axenic conditions was tested using different solid and liquid nutrient media. One strain (YNCX04) produced numerous primordia from which fruiting bodies, 12 mm and 10 mm in length, with grey, convex pilei, and yellow-white, clavate stipes developed between 15 and 30 d after inoculation of fungal mycelium onto a solid medium consisting of mineral salts, thiamine, glucose, potato, an extract of Cunninghamia lanceolata root, and agar. The other strain (YNB200) produced numerous primordia but no sporophores. Strain YNCX04 lost the ability to form fruiting bodies in axenic culture 6 mo after initial isolation but retained the ability to form primordia for up to 18 mo. Based on internal transcribed spacer sequencing data, strains YNB200 and YNCX04 formed a sub-cluster together with four previously designated Boletus edulis strains from China. Phylogenetic analysis placed the Chinese strains closer to B. aestivalis than to European and North American strains of B. edulis, although a 29-bp fragment specific to all the B. aestivalis strains was absent from all the Chinese strains. Furthermore, partial 18S rDNA sequences from strains YNB200 and YNCX04 exhibited 98% similarity with an 18S rDNA sequence from B. edulis strain Be3. Further molecular studies are indicated to more accurately establish the taxonomic positions ofF3 and F4-3, as well as the Chinese strains designated as B. edulis.
On the basis of analyzing the synthesis application of p-q (instantaneous reactive power) theory and unit vector template, a multi-functional grid-connected inverter (MFGCI) with auxiliary services on power quality enhancement in micro-grid (MG) is presented. The novel control strategy for achieving maximum benefits from these MFGCI when installed in AC MG access to 3-phase 4-wire distribution systems by isolated transformer at the point of common coupling (PCC). This kind of inverter can not only deliver the power generation of renewable energy sources (RESs), but also can perform as active power filter (APF) at their PCC as well as can improve the efficiency of inverter and decrease the hardware investment. Finally, both feasibility and effectiveness of this new topologies and control strategies of MFGCI are verified by PSCAD/EMTDC.
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