Three sets of ADCP data obtained from the upper ocean are used to examine possible influence of parametric subharmonic instability (PSI) at ϕc ≈ 13–15°N. Both kinetic‐energy and shear spectra at ϕc reveal that significant peaks appear at subharmonic frequencies of diurnal internal tides. Especially, the 0.5 K1 (subharmonic of K1 tides) can be trapped poleward of its critical latitude (14.52°N), significantly distinguished from wind generated near‐inertial internal waves. Moreover, the enhanced 0.5 K1 motions are more or less subject to a fortnightly spring‐neap circle. Relative to a higher latitude (say 18°N), diurnal motions at ϕc are greatly weakened. In contrast, small vertical‐scale motions in the inertial band and the shear induced by these motions are significantly enhanced. Likely, PSI mechanism plays an important role in these observations, whilst trapped sub‐inertial waves (0.5 K1) may be associated with the presence of negative sub‐inertial eddies.
The ability of two freshly isolated Boletus stains to fruit under axenic conditions was tested using different solid and liquid nutrient media. One strain (YNCX04) produced numerous primordia from which fruiting bodies, 12 mm and 10 mm in length, with grey, convex pilei, and yellow-white, clavate stipes developed between 15 and 30 d after inoculation of fungal mycelium onto a solid medium consisting of mineral salts, thiamine, glucose, potato, an extract of Cunninghamia lanceolata root, and agar. The other strain (YNB200) produced numerous primordia but no sporophores. Strain YNCX04 lost the ability to form fruiting bodies in axenic culture 6 mo after initial isolation but retained the ability to form primordia for up to 18 mo. Based on internal transcribed spacer sequencing data, strains YNB200 and YNCX04 formed a sub-cluster together with four previously designated Boletus edulis strains from China. Phylogenetic analysis placed the Chinese strains closer to B. aestivalis than to European and North American strains of B. edulis, although a 29-bp fragment specific to all the B. aestivalis strains was absent from all the Chinese strains. Furthermore, partial 18S rDNA sequences from strains YNB200 and YNCX04 exhibited 98% similarity with an 18S rDNA sequence from B. edulis strain Be3. Further molecular studies are indicated to more accurately establish the taxonomic positions ofF3 and F4-3, as well as the Chinese strains designated as B. edulis.
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