Müllerian inhibiting substance (MIS), also known as anti-Müllerian hormone (AMH), is a member of the transforming growth factor-β (TGF-β) superfamily that plays an important role in the mesenchymal-epithelial interaction, cell growth and proliferation, extracellular matrix production and tissue remodeling. Previously, we demonstrated that MIS suppressed ovarian cancer cell growth and suggested large-scale genetic elements that could be responsible for anti-neoplastic effects of MIS on ovarian cancer cells. In this study, we demonstrated the expression of MIS type II receptor (MISRII) in the human papillomavirus (HPV)-16-related cervical cancer cell lines CaSki and SiHa, and a non-HPV-related cervical cancer cell line, C33A. We also showed that MIS inhibited growth of cervical cancer cells, and induced cellular apoptosis of C33A. In addition, we identified a characteristic molecular signature of MIS in CaSki cells by using whole genome expression analysis. Of the 1,690 genes that showed significant expression changes by MIS, 21 genes were related to cell cycle; 13 genes to apoptosis; and 52 genes to the cancer pathway. On performing a search for cell cycle pathways in the KEGG pathway database, several gene expressions at the G1/S checkpoint were found. In particular, the expression of p16 and p107 increased and that of E2F2 and E2F3 decreased at an early stage, whereas the expression of E2F4 and E2F5 decreased at a later stage after MIS treatment. These data suggest that MIS produces activity against HPV16-related cervical cancers in vitro, and MIS may also be an effective targeted therapy for HPV16-related cervical cancer. Genetic data obtained here could be useful in determining the treatment strategy of MISR-expressing cervical tumors in the future.
Leiomyosarcomas comprise fewer than 2% of all malignant vaginal neoplasms. Due to their rarity, treatment for vaginal leiomyosarcomas have not been determined. We describe a 66 year old woman with vaginal leiomyosarcoma, which presented as a large palpable mass with vaginal spotting. Complete surgical excision was accomplished and after surgery, she underwent radiation therapy. Tumor recurrence was not detected for the last 5 years and now her general condition is very good. This is a rare case of leiomyosarcoma arising in vagina and we report the results of successful treatment.
Primary uterine non-Hodgkin's lymphoma is a rare malignancy and the most common histological subtype is the diffuse large B-cell lymphoma. This case is a patient underwent surgery for early gastric cancer in November 2007, and determined to be completely cured, and subsequently, during the follow up observation, in pelvic computed tomography, a uterine mass suspicious to be either uterine cervical cancer or uterine sarcoma was observed. She was transferred to department of gynecologic oncology, modified radical hysterectomy and pelvic lymph node dissection were performed. Histologically the uterine mass was diagnosed as diffuse large B-cell lymphoma. Currently she is received the rituximab, cyclophosphamide, adriamycin, vincristine, and prednisone chemotherapy. Thus we report the case with a brief review of the literature. Primary malignant lymphoma in the uterus is very rare, and its diagnosis as well as treatments has not been established. Therefore, in the future, through continuous clinical discussion on this disease, a guideline for the diagnosis and treatment for primary uterine lymphoma should be established.
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