No abstract

There are many limitations to image acquisition, using conventional radiography, of the temporomandibular joint (TMJ) region. The Computed Tomography (CT) scan is a better option, due to its higher accuracy, for purposes of diagnosis, surgical planning and treatment of bone injuries. The aim of the present study was to analyze two protocols of cone beam computed tomography for the evaluation of simulated mandibular condyle bone lesions. Spherical lesions were simulated in 30 dry mandibular condyles, using dentist drills and drill bits sizes 1, 3 and 6. Each of the mandibular condyles was submitted to cone beam computed tomography (CBCT) using two protocols: 1) axial, coronal and sagittal multiplanar reconstruction (MPR); and 2) sagittal plus coronal slices throughout the longitudinal axis of the mandibular condyles. For these protocols, 2 observers analyzed the CBCT images independently, regarding the presence or not of injuries. Only one of the observers, however, performed on 2 different occasions. The results were compared to the gold standard, evaluating the percentage of agreement, degree of accuracy of CBCT protocols and observers' examination. The z test was used for the statistical analysis. The results showed there were no statistically significant differences between the 2 protocols. There was greater difficulty in the assessment of small-size simulated lesions (drill # 1). From the results of this study, it can be concluded that CBCT is an accurate tool for analyzing mandibular condyle bone lesions, with the MPR protocol showing slightly better results than the sagittal plus coronal slices throughout the longitudinal axis.

Improving nutrient utilization efficiency is essential for livestock, given the current scenario of increasing demand for animal protein and sustainable resource use. In this context, understanding the biology of feed efficiency (FE) in beef cattle allows the development of markers for identification and selection of best animals for animal production. Thus, 98 young Nellore bulls were evaluated for FE and at the end of the experiment liver samples from six High Feed Efficient (HFE) and six Low Feed Efficient (LFE) animals were collected for protein extraction, digestion and analysis by HPLC-MS/MS. Data were analyzed for differential abundant proteins (DAPs), protein networks, and functional enrichment. Serum endotoxin was also quantified. We found 42 DAPs and 3 protein networks significantly related to FE. The main pathways associated with FE were: microbial metabolism; biosynthesis of fatty acids, amino acids and vitamins; glycolysis/gluconeogenesis; xenobiotic metabolism and; antigen processing and presentation. Serum endotoxins were significantly higher in LFE animals supporting the results. Therefore, the findings presented here confirmed the altered hepatic metabolism and pronounced hepatic inflammation in LFE animals supporting that the increased bacterial load is at least in part responsible for the hepatic lesions and inflammation in LFE animals.

Objetivou-se comparar equações matemáticas descritas na literatura, identificar a que melhor descreve a produção de leite e estimar alguns componentes da curva de lactação de vacas de alta variabilidade genética, resultante de cruzamento taurino (Bos taurus) x zebuíno (Bos indicus), em sistema de produção semi-extensivo em Sergipe. Foram utilizados 1496 registros de produção de leite. Os controles leiteiros foram realizados em intervalos de 30 dias no período de 2011 a 2012. O ajuste foi feito para a curva média, fazendo-se o uso de quatro modelos matemáticos: modelo de Nelder, 1966 (ND), Wood, 1967 (WD), Bianchini Sobrinho, 1984 (BS) e Wilmink, 1987 (WK). Os critérios utilizados para verificar a qualidade do ajuste para cada função foram: coeficiente de determinação ajustado (Ra2), gráfico de distribuição dos resíduos e porcentagem de desvios entre a produção observada e as estimadas. Os valores médios de Ra2 foram maiores que 0,80 para os modelos empregados, exceto para o modelo WK. Todas as curvas apresentaram padrões típicos. As funções WD e WK subestimaram a produção de leite acumulada aos 305 dias e apresentaram maiores desvios em relação à produção observada. As demais funções apresentaram comportamento semelhante no gráfico de distribuição dos resíduos, no entanto, função polinomial inversa (ND) proporcionou melhor estimativa das variáveis que compõem a curva de lactação de vacas provenientes de cruzamentos de Bos taurus x Bos indicus.

Background Maspin (SERPINB5) is a potential tumor suppressor gene with pleiotropic biological activities, including regulation of cell proliferation, death, adhesion, migration and gene expression. Several studies indicate that nuclear localization is essential for maspin tumor suppression activity. We have previously shown that the EGFR activation leads to maspin nuclear localization in MCF-10A cells. The present study investigated which EGFR downstream signaling molecules are involved in maspin nuclear localization and explored a possible role of cell–cell contact in this process. Methods MCF-10A cells were treated with pharmacological inhibitors against EGFR downstream pathways followed by EGF treatment. Maspin subcellular localization was determined by immunofluorescence. Proteomic and interactome analyses were conducted to identify maspin-binding proteins in EGF-treated cells only. To investigate the role of cell–cell contact these cells were either treated with chelating agents or plated on different cell densities. Maspin and E-cadherin subcellular localization was determined by immunofluorescence. Results We found that PI3K-Akt and JAK2-STAT3, but not MAP kinase pathway, regulate EGF-induced maspin nuclear accumulation in MCF-10A cells. We observed that maspin is predominantly nuclear in sparse cell culture, but it is redistributed to the cytoplasm in confluent cells even in the presence of EGF. Proteomic and interactome results suggest a role of maspin on post-transcriptional and translation regulation, protein folding and cell–cell adhesion. Conclusions Maspin nuclear accumulation is determined by an interplay between EGFR (via PI3K-Akt and JAK2-STAT3 pathways) and cell–cell contact.

O objetivo deste estudo foi avaliar a eventual alteração na posição craniocervical de pacientes orientados na Posição Natural da Cabeça (PNC), após o procedimento de expansão rápida da maxila (ERM). Utilizaram-se telerradiografias em norma lateral, selecionadas do acervo de documentação da Clínica de Ortodontia Preventiva da Faculdade de Odontologia da Universidade de São Paulo, de 20 pacientes, com idades entre 5 a 11 anos (média de 9,0 anos), sendo 7 do gênero masculino e 13 do feminino, obtidas no início do tratamento e imediatamente após a fase ativa da expansão da maxila. Os pacientes eram portadores de atresia da maxila e foram tratados com aparelho disjuntor tipo Biederman modificado. A partir dos cefalogramas pré e pós-ERM foram obtidas as grandezas angulares craniovertical (SN.VER), craniocervical (OPT.SN e CVT.SN), cervicohorizontal (OPT.HOR, CVT.HOR) e o ângulo da curvatura cervical (OPT.CVT). Após análise estatística e interpretação dos resultados obtidos concluímos que a posição da cabeça não apresentou alteração estatisticamente significativa. Não foram observadas diferenças estatisticamente significativas entre os gêneros quando comparadas as fases pré e pós-ERM para as grandezas estudadas. O método de obtenção de telerradiografias laterais em Orientação Natural da Cabeça mostrou-se confiável e reproduzível na amostra estudada, mesmo na presença de intervenção ortodôntica-ortopédica entre as séries de radiografias.

Agradecimentos À Deus pelo dom da vida e por me guiar durante essa caminhada. À minha mãe, Silvanice Alexandre Pereira, por toda garra e amor em todas as fases da minha vida. Aos meus avós (in memorian), José Alexandre Sobrinho e Jovelina Alexandre Pereira, por todo amor e proteção seja nesse ou em um outro plano espiritual. À Madalena Lima Menezes pelo companheirismo e amor durante todos esses anos, sem o seu apoio nada seria possível. Aos meus anjinhos (in memorian) Shelinho e Milinha, por me ensinarem o real significado do amor incondicional, perdão pela ausência durante a minha jornada acadêmica. À minha Tininha por todo o amor dedicado e pelo apoio e incentivo que não requerem palavras para serem sentidos. Ao meu orientador, professor Dr. Joanir Pereira Eler, pela confiança a mim depositada na condução deste trabalho. À Leydiana Duarte Fonseca pela amizade e parceria construída ao longo dos nossos experimentos. Obrigada por toda a ajuda na execução das análises! À Drª Alessandra Fernandes Rosa pelas orientações, confiança e parceria durante a execução desse trabalho. As integrantes do LEACC: Drª Cristina Tschorny Moncau, Drª Mirele Daiana Poleti e Drª Bárbara Silva Vignato, por todos os ensinamentos, trocas de conhecimentos e pela parceria nas discussões sobre as análises. Aos integrantes do GMAB da FZEA/USP: Yuri, Isa, Elisângela, Gerardo, Lenin e D. Vera, pelos momentos compartilhados. Aos amigos da Zootecnia UFS turma de 2007, em especial a Roberta, Shara, Neto, Karen, Camila e Marise, que mesmo distantes sempre se mantiveram presentes. Obrigada pela amizade de uma vida! Aos amigos Lúcio e Maíra pela presença constante em todos os momentos. À Bárbara, Bruno, Zen, Simone e Júnior pelo acolhimento e construção de uma amizade. Ao meu sempre orientador professor Dr. Anselmo Domingos Ferreira Santos pela amizade e por sempre acreditar em meu potencial. Obrigada por todos os ensinamentos, o mérito sempre será nosso! Palavras-chave: biomarcadores, gliconeogênese, Longissimus thoracis, maciez da carne, western blotting ABSTRACT PEREIRA, M. A. Ultimate pH and its alterations in HSP27 and HSP70 concentrations and crossbred cattle skeletal muscle proteomic profile. 2018. 80p. Thesis (PhD)

Were evaluated the microbiological and physicochemical characteristics of fresh and aged semimembranosus muscle from 40 uncastrated male Ile de France lambs with 17.15 ± 1.56 kg of initial body weight. Treatments involved fresh and meat aged for 14 days at 4 ºC from lambs receiving four diets, as follows: control; PM - diet with 20% peanut meal replacing soybean meal; G - diet with 25% glycerin replacing corn; and PMG - diet with 10% peanut meal and 12.5% glycerin. The forage:concentrate ratio was 40:60, and corn silage was used as roughage. The diets had similar values of crude protein and metabolizable energy (17.47% and 2.72 Mcal kg/dry matter). A completely randomized design was adopted in a 4×2 factorial arrangement (four diets × fresh and aged meat). Diets and aging did not favor the development of microorganisms in the meat. There was an interaction effect between diets and aging for pH, color, cooking weight loss (CL), and shear force (SF). The fresh meat from animals fed G was lighter compared with the meat from those consuming PM and PMG (lightness of 38.45, 36.26 and 37.88 respectively). Aging increased lightness (37.63 to 40.87), red intensity (15.12 to 15.93), yellow intensity (2.48 to 3.42) and CL (23.84 to 32.59%), reduced pH (5.66 to 5.45), water holding capacity (61.85 to 58.87%) and SF (22.60 to 16.95 N). By-products from biodiesel and peanut production can be used to replace corn and soybean meal in diets for lambs without compromising their meat quality.