SummaryHuman apolipoprotein (apo) E plays an important role in the metabolism of cholesterol and other lipids. Apo E5 and apo E7 are genetic variants of apo E and have been detected in about 5~ of Japanese patients with hyperlipidemia and ischemic heart disease. The existence of apo E5 and apo E7, however, had not been reported in apparently healthy individuals except for a few family members of the patients with apo E5 or apo E7. It has been suggested that apo E5 and apo E7 are closely related to the development of atherosclerosis. The purpose of this study is to investigate the frequency of apo E5 and apo E7 in apparently healthy Japanese and to analyze serum lipid levels of the individuals with apo E5 or apo E7.The apo E phenotypes of 197 apparently healthy Japanese adults were determined by two-dimensional gel electrophoresis. The gene frequencies ofapo E were: ~3, 0.843; ~4, 0.112; ~2, 0.038; e5, 0.006; ~7, 0.0035. Three out of 187 subjects (1.5~) were found to have apo E5 or apo E7 in heterozygous state. Two of them were heterozygous with apo E3 (apo E3/5 and apo E3/7) and the both had normal serum lipid levels, though they were more than 50 years old. The other individual was heterozygous with apo E2 (apo E2/5) and had mild hypertriglyceridemia. As to myocardial infarction, angina pectoris and cerebral infarction, no clinically abnormal findings were detected in all the three individuals with apo E5 or apo E7.The data suggest that the frequency of the individuals with apo E5 or apo E7 is of the order of 1 X and much higher than that of the homozygotes with apo E2/2 in Japanese. The data also indicate that further genetic, epidemiologic and clinical studies are required to determine whether ~5 and ~7 act as a dominant major gene, as a recessive major gene, or as
SummaryApolipoprotein (apo) CIII Sst-I genotypes and apo AI Msp-I genotypes were investigated in 82 unrelated healthy Japanese, using genomic hybridization analysis with a 2.2 kb fragment of the human apo AI gene. The frequencies of the $2 and M2 alleles were 0.34 and 0.40, respectively, and much higher than those in Caucasians. The alleles identified by the apo CIII Sst-I and apo AI Msp-I polymorphisms were observed to be in linkage disequilibrium (A=0.206+0.012, p<0.001). Three of the four possible haplotypes were identified: the frequencies of the haplotype were S1-M1=0.604, $2-M2=0.341, and S1-M2=0.055. The data indicate that Japanese are characterized by the common presence of the haplotype $2-M2 as compared with Caucasians and that the haplotypes identified by the apo CIII Sst-I and apo AI Msp-I polymorphisms are useful genetic markers for Japanese.
SummaryThis study was performed to investigate whether the ~4 allele of the apolipoprotein (apo) E predisposes Japanese male adults in large cities to hyperlipidemia. The apo E phenotypes and serum total lipid levels were determined using blood samples obtained after an overnight fast from 85 apparently healthy Japanese male civil servants in Tokyo. The frequency of hyperlipidemia was 50.0~ in 20 subjects with the apo E4-positive phenotype and 25.8~ in 62 subjects with the apo E3/3 phenotype. The difference is statistically significant (p<0.05). The mean (_+SD) serum triglyceride levels, which were calculated on the subjects with serum triglyceride lower than 250 mg/dl, was 132.8_+5.1 mg/dl in 18 subjects with the apo E4-positive phenotype and 108.4_+43.0 mg/dl in 58 subjects with the apo E3/3 phenotype. The difference is also statistically significant (p<0.05). The data suggest that the e4 allele might predispose Japanese adults in large cities to hyperlipidemia.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.