As low concentrations of paenibacillin can inhibit Listeria in a food matrix, paenibacillin has potential to be used as a natural food preservative to deter this pathogen’s growth in food, after FDA approval.
Antimicrobials are needed to combat antibiotic- and preservative-resistant bacteria. Spore-forming bacteria are prolific producers of antimicrobials. This assay will speed the discovery of antimicrobials from spore-forming bacteria; these new antimicrobials are urgently needed in food and medicinal applications.
Aim: To detect and characterize novel lantibiotics from a collection of Bacillus spp. using a multifaceted analytical approach.
Methods and Results: A previously completed microassay identified 45 Bacillus isolates with anti-Listeria activity. The isolates were PCR screened using degenerate primers targeting conserved sequences in lanM-type lantibiotics. B. velezensis GF610 produced a PCR product whose sequence, along with genome mining and bioinformatics, guided the liquid chromatographic analysis of strain's cell-free extracts and the mass spectrometry of purified fractions. Results revealed a new amyloliquecidin variant (designated GF610) produced by the strain. Amyloliquecidin GF610 is a two-component lantibiotic with α and β peptides having monoisotopic masses of 3026 and 2451 Da, and molecular formulae C 130 H 191 N 35 O 39 S 5 and C 110 H 158 N 26 O 30 S 4 , respectively. Amyloliquecidin GF610 is active against Listeria monocytogenes, Clostridium sporogenes, Clostridioides difficile, Staphylococcus aureus and Alicyclobacillus acidoterrestris with minimum inhibitory concentrations (MICs) in the range of 0.5-7.0 µmol l -1 .
Conclusions:The proposed multifaceted analytical approach was valuable to provide a deep and proper characterization of a novel bacteriocin, amyloliquecidin GF610, with high antimicrobial activity against Gram-positive bacteria.
Bacillus velezensis GF610 was isolated from soil in Illinois, USA, and found to produce amyloliquecidin GF610, a potent two-component antimicrobial peptide. We report here the GF610 strain draft genome sequence, which contains 4.29 Mb and an overall GC content of 45.91%.
Bacillus velezensis OSY-GA1 is a Gram-positive soil bacterium that exhibits antagonistic activities against Gram-positive and Gram-negative foodborne pathogens. Here, we present the strain’s draft genome, which is 4,009,999 bp long with an average G+C content of 46.2%.
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