Detection and characterization of a rare two‐component lantibiotic, amyloliquecidin GF610 produced by
            <i>Bacillus velezensis</i>
            , using a combination of culture, molecular and bioinformatic analyses

Gerst, Michelle M.; Somogyi, Árpád; Xu, Yang; Yousef, Ahmed E.

doi:10.1111/jam.15290

Cited by 11 publications

(6 citation statements)

References 50 publications

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“…Although many operational Group B. amyloliquefaciens strains are known to produce antimicrobial compounds ( Lv et al, 2020 ; Gerst et al, 2021 ), we sought to discover the direct protective effects of ADS024 and DSM7 in toxin-treated human colonic epithelial cells. C. difficile toxins kill human colonic epithelial cells via apoptosis ( Fiorentini et al, 1998 ; Brito et al, 2002 ), leading to intestinal injury.…”

Section: Resultsmentioning

confidence: 99%

ADS024, a Bacillus velezensis strain, protects human colonic epithelial cells against C. difficile toxin-mediated apoptosis

et al. 2023

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Clostridioides difficile infection (CDI) causes intestinal injury. Toxin A and toxin B cause intestinal injury by inducing colonic epithelial cell apoptosis. ADS024 is a Bacillus velezensis strain in development as a single-strain live biotherapeutic product (SS-LBP) to prevent the recurrence of CDI following the completion of standard antibiotic treatment. We evaluated the protective effects of the sterile filtrate and ethyl acetate extract of conditioned media from ADS024 and DSM7 (control strain) against mucosal epithelial injury in toxin-treated human colonic tissues and apoptosis in toxin-treated human colonic epithelial cells. Ethyl acetate extracts were generated from conditioned culture media from DSM7 and ADS024. Toxin A and toxin B exposure caused epithelial injury in fresh human colonic explants. The sterile filtrate of ADS024, but not DSM7, prevented toxin B-mediated epithelial injury in fresh human colonic explants. Both sterile filtrate and ethyl acetate extract of ADS024 prevented toxin-mediated apoptosis in human colonic epithelial cells. The anti-apoptotic effects of ADS024 filtrate and ethyl acetate extract were dependent on the inhibition of caspase 3 cleavage. The sterile filtrate, but not ethyl acetate extract, of ADS024 partially degraded toxin B. ADS024 inhibits toxin B-mediated apoptosis in human colonic epithelial cells and colonic explants.

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Section: Resultsmentioning

confidence: 99%

ADS024, a Bacillus velezensis strain, protects human colonic epithelial cells against C. difficile toxin-mediated apoptosis

et al. 2023

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“…Amyloliquecidin gene clusters within the SZA14 and SZA16 genomes are present in contigs 14 and 9, respectively. While Gerst et al ( 2022 ) in their study reported the presence of amyloliquecidin in contig 19. So far, the gene cluster for lantibiotic amyloliquecidin has been reported in four strains from different locations: Bacillus spp .…”

Section: Discussionmentioning

confidence: 86%

“…Amyloliquecidin is a relatively new two-component (α and β peptides) lantibiotic (Gerst et al 2022 ). It is active against most of the tested gram-positive species, which include Listeria monocytogenes, Clostridium sporogenes , Clostridioides difficile, S. aureus (Van Staden et al 2016 ) , and Alicyclobacillus acidoterrestri (Gerst et al 2022 ) . Amyloliquecidin gene clusters within the SZA14 and SZA16 genomes are present in contigs 14 and 9, respectively.…”

Section: Discussionmentioning

confidence: 99%

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Whole-genome sequencing and antimicrobial potential of bacteria isolated from Polish honey

Hafeez,

Pełka,

Buzun

et al. 2023

Appl Microbiol Biotechnol

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The aim of this study was the whole-genome analysis and assessment of the antimicrobial potential of bacterial isolates from honey harvested in one geographical location—the north of Poland. In total, 132 strains were derived from three honey samples, and the antimicrobial activity of CFAM (cell-free after-culture medium) was used as a criterion for strain selection and detailed genomic investigation. Two of the tested isolates (SZA14 and SZA16) were classified as Bacillus paralicheniformis, and one isolate (SZB3) as Bacillus subtilis based on their ANI and phylogenetic analysis relatedness. The isolates SZA14 and SZA16 were harvested from the same honey sample with a nucleotide identity of 98.96%. All three isolates have been found to be potential producers of different antimicrobial compounds. The secondary metabolite genome mining pipeline (antiSMASH) identified 14 gene cluster coding for non-ribosomal peptide synthetases (NRPs), polyketide synthases (PKSs), and ribosomally synthesized and post-translationally modified peptides (RiPPs) that are potential sources of novel antibacterials. The BAGEL4 analysis revealed the presence of nine putative gene clusters of interest in the isolates SZA14 and SZA16 (including the presence of six similar clusters present in both isolates, coding for the production of enterocin Nkr-5-3B, haloduracin-alpha, sonorensin, bottromycin, comX2, and lasso peptide), and four in B. subtilis isolate SZB3 (competence factor, sporulation-killing factor, subtilosin A, and sactipeptides). The outcomes of this study confirm that honey-derived Bacillus spp. strains can be considered potential producers of a broad spectrum of antimicrobial agents. Key points • Bacteria of the genus Bacillus are an important component of honey microbiota. • Honey-derived Bacillus spp. strains are potential producers of new antimicrobials.

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“…The anti- Listeria molecule of SF214 is, therefore, highly specific and for this reason peculiar with respect to other AMPs that have a broad spectrum of action [ 1 – 3 ]. Examples are the recently discovered Amyloliquecidic GF610 of B. velenzensis [ 17 ] and a molecule produced by B. amyliquefaciens JFL21 [ 18 ], both active against Listeria but also against several Gram-positives and the latter also against some fungal pathogens. For its specificity and its proteinaceous nature the SF214 molecule will be therein indicate as s pecific a nti- Listeria m onocytogenes p eptide (SAMP) and all further analyses performed with L. monocytogenes ATCC 7644 as the target bacterium.…”

Section: Resultsmentioning

confidence: 99%

An antimicrobial peptide specifically active against Listeria monocytogenes is secreted by Bacillus pumilus SF214

et al. 2022

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Background Members of the Bacillus genus produce a large variety of antimicrobial peptides including linear or cyclic lipopeptides and thiopeptides, that often have a broad spectrum of action against Gram-positive and Gram-negative bacteria. We have recently reported that SF214, a marine isolated strain of Bacillus pumilus, produces two different antimicrobials specifically active against either Staphylococcus aureus or Listeria monocytogenes. The anti-Staphylococcus molecule has been previously characterized as a pumilacidin, a nonribosomally synthesized lipopetide composed of a mixture of cyclic heptapeptides linked to fatty acids of variable length. Results Our analysis on the anti-Listeria molecule of B. pumilus SF214 indicated that it is a peptide slightly smaller than 10 kDa, produced during the exponential phase of growth, stable at a wide range of pH conditions and resistant to various chemical treatments. The peptide showed a lytic activity against growing but not resting cells of Listeria monocytogenes and appeared extremely specific being inactive also against L. innocua, a close relative of L. monocytogenes. Conclusions These findings indicate that the B. pumilus peptide is unusual with respect to other antimicrobials both for its time of synthesis and secretion and for its strict specificity against L. monocytogenes. Such specificity, together with its stability, propose this new antimicrobial as a tool for potential biotechnological applications in the fight against the dangerous food-borne pathogen L. monocytogenes.

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Detection and characterization of a rare two‐component lantibiotic, amyloliquecidin GF610 produced by Bacillus velezensis , using a combination of culture, molecular and bioinformatic analyses

Cited by 11 publications

References 50 publications

ADS024, a Bacillus velezensis strain, protects human colonic epithelial cells against C. difficile toxin-mediated apoptosis

ADS024, a Bacillus velezensis strain, protects human colonic epithelial cells against C. difficile toxin-mediated apoptosis

Whole-genome sequencing and antimicrobial potential of bacteria isolated from Polish honey

An antimicrobial peptide specifically active against Listeria monocytogenes is secreted by Bacillus pumilus SF214

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