Draft Genome Sequence of Bacillus velezensis GF610, a Producer of Potent Anti- Listeria Agents

Abstract

Bacillus velezensis GF610 was isolated from soil in Illinois, USA, and found to produce amyloliquecidin GF610, a potent two-component antimicrobial peptide. We report here the GF610 strain draft genome sequence, which contains 4.29 Mb and an overall GC content of 45.91%.

Detection and characterization of a rare two‐component lantibiotic, amyloliquecidin GF610 produced by Bacillus velezensis , using a combination of culture, molecular and bioinformatic analyses

“…Bacteriocin mining software (BAGEL3; van Heel et al, 2013, BAGEL4; van Heel et al, 2018) was used to reveal amyloliquecidin synthetic gene cluster and post‐translation‐related genes in B . velezensis GF610 genome (Gerst et al, 2017). Predicted masses based on the peptide sequences obtained from bioinformatics analysis were compared to those obtained by mass spectrometry of purified amyloliquecidin fractions.…”

“…The genome of the isolate with potential for novel lantibiotic genes (B. velezensis GF610) was sequenced using Illumina MiSeq platform, and results were published as a draft genome announcement (Gerst et al, 2017). Bacteriocin mining software (BAGEL3; van Heel et al, 2013, BAGEL4;van Heel et al, 2018) was used to reveal amyloliquecidin synthetic gene cluster and post-translation-related genes in B. velezensis GF610 genome (Gerst et al, 2017). Predicted masses based on the peptide sequences obtained from bioinformatics analysis were compared to those obtained by mass spectrometry of purified amyloliquecidin fractions.…”

Simultaneous Production of Β-glucanase and Protease From Bacillus Velezensis Strain Identified in the Manure of Piglets.

“…Bacillus velezensis was rstly isolated from the the mouth of the river Vélez in Málaga (Southern Spain), has originally described by Ruiz-García et al (14). Many currently known Bacillus velezensis were isolated from plant roots and soil (15,16). To the best of our knowledge, this is the rst report that Bacillus velezensis has been isolated from the manure of piglets.…”

The “Three Cs” of Novel Antibiotic Discovery and Production through Synthetic Biology: Biosynthetic Gene Clusters, Heterologous Chassis, and Synthetic Microbial Consortia

“…Recent examples of antiSMASH being utilized for antibiotic BGC discovery include the analysis of the genome of Streptomyces albolongus YIM101047 by Yin et al, yielding clusters with high or moderate similarity to multiple known antibiotic gene clusters for clavulanic acid, collismycin A, frontalamides, kanamycin, streptomycin, and streptothricin; the identification of the BGC of a two‐component lantibiotic anti‐ Listeria agent in the genome of Bacillus velezensis GF610, hypothesized to be similar to previously described amyloliquecidin AD 2; and the discovery of a novel circularin‐like bacteriocin cluster from the genome of Geobacillus stearothermophilus DSM 458 . antiSMASH was also utilized by Nielsen et al to mine the genomes of 24 Penicillium species to yield 1317 putative BGCs for further analysis—a reminder that antibiotic gene cluster discovery is not solely limited to bacterial genomes …”

“…[10] With advances in bioinformatics tools in the genomic era, the mining of microbial genomes for antibiotic production genes-arranged in the genome as "biosynthetic gene clusters" (BGCs)-is beginning to yield novel natural products with antimicrobial potential. [21][22][23] Previously undiscovered due to their poor expression in standard culture conditions, these clusters were described as "silent," "orphan," or "cryptic." [24,25] As the cost of genome sequencing decreases rapidly (down to 1121 USD per 3000 Mb as of July 2017), [26] the availability of microbial genome sequences increases: ≈130 000 prokaryotic genome sequences have been uploaded to National Center for Biotechnology Information (NCBI) as of the time of writing.…”