The present study focused on the beneficial effects of Lactococcus lactis subsp lactis CNRZ 1427 with possible use as a therapeutic agent against Sal-monella sp.; also we have proposed different therapeutic possibilities of our situation against a pathogen Salmonella sp. We have conducted two tests In Vitro and In Vivo; where it is noted that treatment in the presence of this lac-tic strain is effective since it causes a remarkable decrease of the pathogen agent. At the end, the effectiveness of this lactic strain was confirmed by testing for resistance to gastrointestinal conditions (pH, bile salts and diges-tive enzymes).
Salmonella enterica serovar typhimurium is the first cause identified of food poisoning in humans; new data indicate that the resistant strains of Salmonella sp. can be inhibited by a bacterial population present in foods have been called probiotics. Our study was conducted on Swiss albino mice in order to know the effect of probiotics or biotherapeutic agents, and suggest the different therapeutic possibilities of intestinal complications related to the presence of Salmonella enterica serovar typhimurium; Reference strains, Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus acidophilus and Lactobacillus paracasei in combination with Bifidobacterium sp. were tested for their health benefits. The number of Salmonella enterica serovar typhimurium decreases in intestine, in the stomach and in the liver for group 2, 3 in case of preventive treatment and group 4, 5 in case of a therapeutic treatment due to the use of a culture of probiotics, in comparison with group 1 where we recorded a high number. The bio-therapeutic treatment was finally confirmed by macroscopic and microscopic observations of different target organs before and after treatment for the experiment period.
This study focused on the benefices effects of micro-organisms called probiotics that is Lactococcus lactis sbsp cremoris CNRZ 107, and propose different therapeutic possibilities of our situation against a pathogen Salmonella sp.We conducted two tests in vitro and in vivo; where it was noted that treatment in the presence of this lactic strain is effective because it causes a remarkable decrease of the pathogen agent.In the end, the effectiveness of this lactic strain was confirmed by testing for resistance to gastrointestinal conditions (pH, bile salts and digestive enzymes).
Within the framework of our research of medicinal plants a phytochemical and biological study of Asphodelus tenuifolius has been conducted. The study consisted of evaluating the antifungal activities of most of the chemical constituents extracted from leaves by many solvents and tested on Aspergil-lus flavus, A. parasiticus, A. ochraceus, A. fumigatus, A. niger, Penicillium expansum, Fusarium oxysporum and Alternaria sp.The inhibitory activity of phytochemicals was evaluated on fungi isolating from the imported France soft wheat marketed in Algeria, according to two methods: in agar medium and in Y.E.S. medium. The antifungal activity of the four extracts was evalu-ated against eight mycotoxicogenic fungi, by using agar dilution method. The phytochemical analysis conducted on A. tenuifolius leaves and roots re-vealed the presence of many compounds and the antifungal test results indi-cated a great potential of antifungal activity. Aspergillus species proved the most sensitive but F. oxysporum was the most resistance fungus. These re-sults explain that we can use A. tenuifolius as a natural antifungal agent to inhibit and to control mycotoxicogenic fungi.
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