There are many methods used to fabricate the scaffolds for tissue regeneration, among which freeze casting has attracted a great deal of attention due to the capability to create a unidirectional structure. In this study, polycaprolactone (PCL) scaffolds were fabricated by freeze-casting technology in order to create porous microstructure with oriented open-pore channels. To induce biomineralization, and to improve hydrophilicity and cell interactions, mussel-inspired polydopamine (PDA) was coated on the surface of the freeze-cast PCL constructs. Then, the synergistic effects of oriented microstructure and deposited layer on efficient reconstruction of injured bone were studied. Microscopic observations demonstrated that, the coated layer did not show any special change in lamellar microstructure of the scaffolds. Water-scaffold interactions were evaluated by contact angle measurements, and they demonstrated strong enhancement in the hydrophilicity of the polymeric scaffolds after PDA coating. Biodegradation ratio and water uptake evaluation confirmed an increase in the measured values after PDA precipitation. The biomineralization of the PDA-coated scaffolds was characterized by field-emission scanning electron microscopy (FE-SEM), energy dispersive X-ray (EDX) and X-ray diffraction (XRD). Obtained results confirmed biomineralization of the constructs after a 28-day immersion in a simulated body fluid (SBF) solution. Mechanical analysis demonstrated higher compressive strength after PDA coating. L929 fibroblast cell viability and attachment illustrated that PDA-coated PCL scaffolds are able to support cell adhesion and proliferation. The increased secretion of alkaline phosphatase (ALP) after culturing osteosarcoma cell lines (MG-63) revealed the initial capability of scaffolds to induce bone regeneration. Therefore, the PDA-coated scaffolds introduce a promising approach for bone tissue engineering application.
Although many efforts have been made to regenerate the bone lesions, existing challenges can be mitigated through the development of tissue engineering scaffolds. However, the weak control on the microstructure of constructs, limitation in preparation of patient-specific and multilayered scaffolds, restriction in the fabrication of cell-laden matrixes, and challenges in preserving the drug/growth factors' efficacy in conventional methods have led to the development of bioprinting technology for regeneration of bone defects. So in this review, conventional 3D printers are classified, then the priority of the different types of bioprinting technologies for the preparation of the cell/growth factor-laden matrixes are focused. Besides, the bio-ink compositions, including polymeric/hybrid hydrogels and cell-based bio-inks are classified according to fundamental and recent studies. Herein, different effective parameters, such as viscosity, rheological properties, cross-linking methods, biodegradation biocompatibility, are considered. Finally, different types of cells and growth factors that can encapsulate in the bio-inks to promote bone repair are discussed, and both in vitro and in vivo achievement are considered. This review provides current and future perspectives of cell-laden bioprinting technologies. The restrictions and challenges are identified, and proper strategies for the development of cell-laden matrixes and high-performance printable bio-inks are proposed.
Hyaluronic acid (HA) is of immense importance to biomaterials science and biomedical engineering. It is finding applications in diverse areas of bioengineering ranging from scaffolds for disease modelling to tissue culture for reconstruction. This review focuses on recent research on the role of HA as a photo-cross-linked bioink and its importance in combating bone and cartilage-related disease, injury and disorders. Photo chemical modifications and 3D fabrication technologies employed to produce HA-modified materials are analysed to provide a fundamental understanding of the structure-function-property relationships that influence printability, shape fidelity and biological performance both invitro and in-vivo. The article concludes with a future vision for HA-based bioinks and their deployment in light-based bioprinting technologies for bone and cartilage repair.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.