Analysis of engagement of serotonin-modulating anticonsolidation protein (SMAP) in formation of drug addiction to morphine in self-administration model in the rats via application of the anti-SMAP antibodies MethodsIndirect ELISA-test, morphine self-administration model, conditioned model of alternative running, conditioned model of instrumental differentiation. ResultsUpregulation of SMAP by 67% in the brain cingulate cortex of the rats with stable level of morphine intake, while no changes of SMAP level were noticed in hypothalamus. Intramuscular administration of the anti-SMAP antibodies to the rats with stable level of morphine intake leads to significant suppression of morphine consumption for 8 days. Administration of non-immune γ-globulins does not have any effect on morphine consumption. Intra-cerebral administration of the anti-SMAP antibodies leads to significant acceleration and strengthening of memory formation in complicated conditioned alternative running and instrumental differentiation models. ConclusionApplication of the anti-SMAP antibodies induces suppression of elaborated drug addiction in the rats, first, through blockade of intra-cellular transduction of serotonin signal in the concerned nervous cells and, second, due to formation of negative memory on inefficacy of lever pressing to get bright positive emotions after morphine self-administration.
The article concerns application of embryonic model of Xenopus laevis for elaborating preparations directed either to suppression of cell proli fe ration, or, in opposite, to promoting forced cell differentiation. In the 1 st series of studies the dynamics of the serotonin-modulating anticonsolidation protein (SMAP), being in linear rela tions with serotonin, in the embryos of Xenopus laevis throughout the stages of embryo genesis and metamorphosis was pursued with application of indirect ELISA-test. Beginning from the blastula stage till the end of the neurula its level remained unchanged. Thereafter continuous downregulation of the SMAP level, inter rupted as a slight upregulation between 37 th stage and onset of the 39 th stage, was observed. In the 2 nd series of studies incubation of the embryos of Xenopus laevis on the blastula and gastrula stages in fresh water containing SMAP at a dose of 50 and 100 μg/ml led to delay in development and, finally, to death of all the em bryos within 4 days of observation. In the 3 rd series of studies blocking of SMAP activity with the anti-SMAP poly clonal antibodies at a dose of 50 μg/ml in the embryos of Xenopus laevis, being on the 37 th stage of deve lopment, resulted in their passing ahead (by two stages earlier) of the meta morphosis stage relatively to the rate of passing of this stage by the animals of the intact and control groups. So, if on the initial stages of embryogenesis SMAP realizes cytostatic activity, on the meta morphosis stage blocking its activity with antibodies, conversely, leads to passing ahead cell differen tiation.Correspondence to: Mekhtiev AA,
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.