Application of embryonic models for elaboration of anti-carcinogenic preparations of desired action

Abstract

The article concerns application of embryonic model of Xenopus laevis for elaborating preparations directed either to suppression of cell proli fe ration, or, in opposite, to promoting forced cell differentiation. In the 1 st series of studies the dynamics of the serotonin-modulating anticonsolidation protein (SMAP), being in linear rela tions with serotonin, in the embryos of Xenopus laevis throughout the stages of embryo genesis and metamorphosis was pursued with application of indirect ELISA-test. Beginning from the blastula stage till the end of the neurula its level remained unchanged. Thereafter continuous downregulation of the SMAP level, inter rupted as a slight upregulation between 37 th stage and onset of the 39 th stage, was observed. In the 2 nd series of studies incubation of the embryos of Xenopus laevis on the blastula and gastrula stages in fresh water containing SMAP at a dose of 50 and 100 μg/ml led to delay in development and, finally, to death of all the em bryos within 4 days of observation. In the 3 rd series of studies blocking of SMAP activity with the anti-SMAP poly clonal antibodies at a dose of 50 μg/ml in the embryos of Xenopus laevis, being on the 37 th stage of deve lopment, resulted in their passing ahead (by two stages earlier) of the meta morphosis stage relatively to the rate of passing of this stage by the animals of the intact and control groups. So, if on the initial stages of embryogenesis SMAP realizes cytostatic activity, on the meta morphosis stage blocking its activity with antibodies, conversely, leads to passing ahead cell differen tiation.Correspondence to: Mekhtiev AA,