The current research seeks to examine the anticancer effect of polyisoprenoids from mangrove palm, Nypa fruticans, leaves in WiDr cells by analyzing the cell cycles of cancer and regulating the expression of p53, epidermal growth factor receptor (EGFR), PI3K, AKT1, and mammalian target of rapamycin (mTOR) genes by using the reverse transcription-polymerase chain reaction (RT-PCR). An inhibited cell cycle analysis was conducted using the flow cytometry, and the upregulation or downregulation of the expression of p53, EGFR, PI3K, AKT1, and mTOR genes was obtained using RT-PCR. The data were then statistically analyzed using one-way analysis of variance by a post hoc test, a parametric statistical analysis using Tukey’s honest significant difference. Polyisoprenoids in N. fruticans extracts worked as chemotheraupetic in the G0-G1 cycle is 79.0%, however, with positive control 5-fluorouracil as 88.1% and are carried out by the specific upregulation of the expression of the p53 gene and the downregulation of the expression of the EGFR, PI3K, AKT1, and mTOR genes. This study can also explain the significant pharmacological properties of the leaves of the species N. fruticans that work specifically in the G0-G1 phase to upregulate the expression of the p53 gene and downregulate the expression of the EGFR, PI3K, AKT1, and mTOR genes. This study also revealed polyisoprenoid (100% dolichol), which blocked the growth and development of WiDr colon cancer cells.
BACKGROUND: Mangroves secondary metabolites are mostly consisting of sterols, ubiquinones, isoprenoids, and polyisoprenoids. Polyisoprenoid is divided into two types, namely, polyprenol and dolichol, which has been reported to have biological and pharmacological activities. AIM: This research was aimed to analyze apoptosis 48 h with double staining and immunocytochemistry (ICC) 48 h of P53 and cyclooxygenase-2 (COX-2) gene expression from chemical constituents of dolichol in three mangrove leaves of Ceriops tagal, Nypa fruticans, and Rhizophora mucronata. METHODS: Apoptosis with the double-staining method was employed to analyze the genes expression in growth and development of cancer cells, P53, and COX-2 with ICC and flow cytometry method. The data were statistically analyzed using one-way ANOVA parametric statistical analysis followed by Duncan’s test. RESULTS: The result revealed that the increased apoptosis of samples C. tagal was 70% fluorescence orange, while N. fruticans and R. mucronata were 35% and 30% fluorescence orange, respectively. However, it was compared with the positive control; it produced orange fluorescent as much as 75%, suggesting that C. tagal have a position similar to 5-FU. Predominance dolichol in N. fruticans and C. tagal leaves led the expression gene of p53 to have 1.57% M1 phase, indicating the domination in G0-G1 phase (70–80%). Inhibit the expression for 48 h in p53 and COX-2 showing that n-hexane extract of C. tagal had the most percentage (80.733 ± 0.11%) to upregulate the p53 and less percentage (20.16 ± 1.19%) to downregulate the COX-2, indicating positive extract belong to N. fruticans and R. mucronata leaves. CONCLUSION: The present study confirmed the pharmacological properties of dolichol from three mangrove leaves as an anticancer of tumor suppressor genes and significantly proliferated of cancer cell growth from mangrove leaves.
Mangrove plants produce polyisoprenoid alcohol. The polyisoprenoid consists of polyprenol and dolichol, which in pharmacological activity act as anticancer agents. The major polyisoprenoid compound of mangrove plants Ceriops tagal and Rhizophora mucronata was reported as dolichol. The present study was conducted to examine the anticancer effects of dolichol from C. tagal and R. mucronata leaves on WiDr cells and cell cycle-related cancer for 24 h and to evaluate the regulation of five genes, p53, EGFR, PI3K, Akt, and mTOR. The inhibited cell cycle was analysed by flow cytometry and the gene expression of p53, EGFR, PI3K, Akt, and mTOR was determined using reverse transcription-polymerase chain reaction (RT-PCR) method. Dolichol from C. tagal was more effective than that from R. mucronata,where it worked on the G0/G1 cycle for 87.94% and 82.36%, respectively, and regulated positive control 5-FU on the G0/G1 cycle (88.12%), S (9.52%) and G2-M (6.42%). The upregulation (p53) and downregulation (EGFR)contributed to the contracting cell cycle of colon cancer cells (WiDr) in PI3K, Akt and mTOR genes. To summarise, the current study suggests significant pharmacological properties of dolichols in C. tagal and R. mucronata leaves, which worked explicitly in the G0/G1 phase.
Objectives: The study aims to examine the anticancer effect of polyisoprenoid of Nypa fruticans, Ceriops tagal, and Rhizophora mucronata leaves in WiDr cells by evaluating the regulation of p21 and Akt 2 gene. Design: Nypa fruticans, Ceriops tagal, and Rhizophora mucronata leaves were dried and extracted with n-hexane, analyzed the increase or decrease in regulation of p21 gene and Akt 2 expression which was determined the Reverse Transcription Polymerase Chain Reaction (RT-PCR) method. Interventions: The variable that was intervened in this study was the 3 sample mangrove leaves. Main outcome measure: The main measurement results in this study were to study n-hexane extracts of mangrove leaves able to suppress the expression of p21 and Akt 2 genes so that cancer cell growth is inhibited. Results: n-hexane extract of Ceriops tagal leaves was more effective than Nypa fruticans and Rhizophora mucronata, in which there was up-regulated (p21) of 1.19 and down regulated (Akt 2) of 0.78 on colon cancer cells (WiDr). N-hexane extract of mangrove leaves has cancer chemoprevention activity with up-regulated and down-regulated on WiDr cells, in which the sample was more effective than n-hexane extract of Ceriops tagal leaves. Conclusion: N-hexane extract of mangrove leaves had cancer chemoprevention activity with up-regulated and down-regulated on WiDr cells, in which the sample was more effective than n-hexane extract of Ceriops tagal leaves.
BACKGROUND: Mangrove plants distributed in the intertidal of the tropical and subtropical region including in North Sumatra, Indonesia. The production of secondary metabolite compounds is well known to mangroves. Characterisation of prominent compounds from mangrove plants such as genus of Avicennia is required to explore for their biological and pharmacological properties of these compounds. AIM: The purpose of this research was to analyse the prominent secondary metabolites through the characterisation of phytochemical, physicochemical, and microscopic of the mangrove genus Avicennia leaves, particularly Avicennia alba, A. lanata, A. marina, and A. officinalis. METHODS: Phytochemical screening was carried out on Avicennia spp leaves to the established process. Physicochemical characters of mangrove leaves were investigated by simplicial powder consisting of moisture content, water-soluble, ethanol-soluble, ash content and ash soluble acid according to the WHO formula. Microscopic analysis on the simplicial powder was carried out based on the WHO procedure. RESULTS: The result showed that physicochemical feature displays diversity among the species and important findings on the water concentration was less than 10% as a prerequisite for the drug. The phytochemical search of simplified grain also depicted divergence among the species, only alkaloid, saponin, and triterpenoid or phytosterol were found entirely in Avicennia spp leaves. Microscopic search found a similar type of stoma in Avicennia spp leaves, namely diacytic. CONCLUSION: The prominent secondary metabolites in Avicennia spp leaves consisting of alkaloid and saponin in simplicial and triterpenoid/sterol was either in simplicial or hexane extract. The present study may provide significant pharmacological properties from mangrove Avicennia genus green foliages, which could accelerate another prospect for non-wood mangrove utilisation. nus green foliages, which could accelerate another prospect for non-wood mangrove utilization.
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