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Introduction. Coronavirus 2019 disease (COVID-19), caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), has spread to more than 200 countries worldwide. We aimed to present acute pancreatitis (AP) cases caused by SARS-CoV-2 viral infection. Methods. The study was conducted retrospectively between April 2020 and June 2020 in Necmettin Erbakan University Meram, Medical Faculty Hospital, and 150 hospitalized patients diagnosed with COVID-19 were included. The degree of acute pancreatitis was determined according to the Atlanta classification. Organ failures of the patients were evaluated in terms of respiratory, cardiovascular, and nephrology according to the modified Marshall scoring (MMS) system, and CTSI (Balthazar score) and Imrie score were determined. Modified   Marshall   score ≥ 2 was considered organ failure. Results. A total of 29 patients were diagnosed with acute pancreatitis. All 29 patients with pancreatitis had respiratory failure during hospitalization. After the diagnosis of pancreatitis, there was no change in respiratory failure. According to the Atlanta classification, 19 patients had mild AP and 10 patients had moderate AP. Patients with acute pancreatitis were scored according to the CTSI (Balthazar score), and there were no patients with ≥6 severe pancreatitis. The CTSI score of 4 patients was 3. In addition, the Imrie score of the patients was determined and 8 patients with Imrie   score ≥ 3 were identified. Conclusion. The rate of pancreatic damage in SARS-CoV-2 infection was found to be 19% ( n = 29 ) in our study. In our study, we highlight acute pancreatitis as a complication associated with COVID-19 and the importance of pancreatic evaluation in patients with COVID-19 and abdominal pain is demonstrated.

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Introduction and aim. Mutation(s) in the gene encoding the CD19 molecule affect CD19 protein expression and primary immunodeficiency (PID) occurs. The PCR-RFLP method, which is faster and cheaper than other mutation detection methods, is rarely used in the diagnosis of PID. The study aimed to genetically identify CD19 deficiency, which is a PID, using the PCR-RFLP method. Material and methods. A total of 8 patients and two healthy controls were included in the study and the relevant region genotypes in the CD19 gene were determined by performing PCR-RFLP analysis. Results. The index case, newborn baby and mother were also included in the study. It was determined that the index case (P6) was homozygous mutant, the newborn baby (P7) and mother (P8) had heterozygous genotype. Based on this situation, one child (P1) was found to be homozygous mutant, mother (P2), father (P3) and other children (P4 and P5) had heterozygous genotype in the family, which was determined to be related to the first case. Conclusion. In our study, it has been shown that PCR-RFLP is a method that can be used in the diagnosis of PID by determining genotypes using PCR-RFLP, and especially in terms of rapid genetic testing of family screenings.

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ÖzB lenfosit yüzeyinde bulunan CD19 molekülü olgun B hücrelerinde CD21, CD81, CD225 ile birlikte CD19 kompleksini oluşturur ve antijen uyarısı ile birlikte B lenfosit aktivasyonunu düzenler. CD19 molekülünü kodlayan gende oluşacak mutasyonlar, CD19 protein ekspresyonunu etkilemekte ve primer immün yetmezlik (PIY) tablosu ortaya çıkmaktadır. Bu çalışmada CD19 eksikliği tanısıyla izlediğimiz hastamızın 3 aylık bebeğinin, RFLP yöntemiyle aynı gen mutasyonu yönünden değerlendirilmesi amaçlanmıştır. Çalışmaya bilinen CD19 mutasyonlu hasta, bu hastanın yeni doğan bebeği ile hastanın annesi ve iki sağlıklı kontrol dahil edilmiştir. Mutasyon analizi için ilk olarak CD19 genindeki mutasyon bölgesini kapsayan primerler dizayn edilip PZR-RFLP işlemi gerçekleştirilmiştir. Oluşan DNA fragmentleri agaroz jel elektoroforezinde görüntülenip genotiplemesi yapılmıştır. Hastamızın CD19 geninin ekzon 6'da saptanan çerçeve kayması mutasyonu (c.973_973insA) yönünden bebeğinin ve annesinin taşıyıcı olduğu PZR-RFLP yöntemi ile gösterilmiştir. PZR-RFLP yönteminin, bilinen mutasyonların belirlenmesinde kullanılabilecek ucuz, hızlı ve güvenilir bir yöntem olduğu kanısındayız.

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