Introduction With recent advances in technology, many manual tests are being replaced by automated devices due to a wide range of advantages. One of these tests is the erythrocyte sedimentation rate (ESR) test that is used to determine inflammatory activity. This study aimed to evaluate the agreement between the Starrsed Interliner sedimentation device and the gold standard method, that is the Westergren method, used in ESR measurement. Methods One hundred fifty‐one patients who presented to Gaziantep University Faculty of Medicine, Şahinbey Training and Research Hospital were included in this study. ESR values were measured simultaneously within 2 hours using the ESR analyzer Starrsed Interliner device and the gold standard method of measuring ESR, that is the Westergren method, from blood samples collected from the same patients in EDTA and citrate tubes. Results Agreement between the results from the Starrsed Interliner device and the Westergren method was evaluated using the Intraclass Correlation method. Consequently, a poor correlation was observed at values <20 mm/h, a moderate correlation was observed at values 20 to 80 mm/h and >80 mm/h, and an excellent correlation was observed when all results were considered. Method comparison was conducted according to the Passing‐Bablok regression analysis (y = −1.50 + 0.75x) (P < .0001). The mean difference between the two methods was 10.1 according to the Bland‐Altman analysis. Conclusions Despite the advantages of the Starrsed Interliner device, such as lower laboratory workloads, lower costs and turnaround time, the difference between the two methods, as found in this study, may lead to different clinical interpretations for results in some patient.

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Background/aim: To evaluate the protective effect of melatonin on ovarian ischemia reperfusion injury in a rat model. Materials and methods: Forty-eight rats were separated equally into 6 groups. Group 1: sham; Group 2: surgical control with 3-h bilateral ovarian torsion and detorsion; Group 3: intraperitoneal 5% ethanol (1 mL) just after detorsion (as melatonin was dissolved in ethanol); Group 4: 10 mg/kg intraperitoneal melatonin 30 min before 3-h torsion; Group 5:10 mg/kg intraperitoneal melatonin just after detorsion; Group 6:10 mg/kg intraperitoneal melatonin 30 min before torsion and just after detorsion. Both ovaries and blood samples were obtained 7 days after detorsion for histopathological and biochemical analysis.Results: In Group 1, serum levels of total oxidant status (TOS) (μmol H2O2 equivalent/g wet tissue)were significantly lower than in Group2 (P = 0.0023), while tissue TOS levels were lower than in Group 3 (P = 0.0030). Similarly, serum and tissue levels of peroxynitrite in Group 6were significantly lower than those ofGroup 2 (P = 0.0023 and P = 0.040, respectively). Moreover, serum oxidative stress index (OSI) (arbitrary unit) levels were significantly increased in Group 2 when compared to groups 1 and 6 (P = 0.0023 and P= 0.0016, respectively) and in Group 3 with respect to groups 1, 4, 5, and 6 (P = 0.0023, P = 0.0026, P = 0.0008, and P = 0.0011, respectively). Furthermore, there was a significant decrease in histopathological scores including follicular degeneration, vascular congestion, hemorrhage, and inflammation in the melatonin and sham groups in comparison with control groups. Additionally, primordial follicle count was significantly higher in Group 6 than in Group 2 (P = 0.0002).Conclusion: Melatonin attenuates ischemia reperfusion damage in a rat torsion/detorsion model by improving histopathological and biochemical findings including OSI and peroxynitrite.

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This study aims to determine and compare oxidative stress responses in muscle and heart tissues of rainbow trout (Oncorhynchus mykiss) resulting from exposure (24, 48 and 96 hours) to sub-lethal concentrations of cadmium (1, 3 and 5 mg/L). Malondialdehyde (MDA) and Peroxynitrite (ONOOˉ) parameters were studied to determine oxidative/nitrosative stress, respectively. The MDA and ONOO− values of heart and muscle tissues showed statistically significant (P<0.05) differences between each other in terms of both hours and doses. The difference in muscle tissue ONOO− and MDA values between control and other groups was found to be statistically significant (P<0.05). Similarly difference in heart tissue ONOO− and MDA values between control and other groups was found to be statistically significant (P<0.05). For both parameters as the amount of the administered dose increased, the average of the ONOO− and MDA values obtained increased significantly. As a result, it was determined that cadmium increases ONOO− and MDA levels in heart and muscle tissues, causes stress and is toxic to fish even in small doses.

Objectives Our aim in this study was to examine the effects of different anti-inflammatory treatment strategies on the process of atherosclerosis, which is an important cause of mortality in ankylosing spondylitis (AS) patients, by examining the possible effect of treatments on inflammation, lipid profile and oxidative stress parameters in patients with AS. Methods In this study, 32 healthy volunteers served as the control group in addition to a total of 76 AS patients who were divided into two groups according to whether they were receiving anti-TNF medications or not. We assessed the levels of inflammation, oxidative stress, lipid profile parameters, and oxidized low-density lipoprotein (oxLDL). The atherogenic index of plasma (AIP), non-HDL cholesterol levels, BASDAI, and BASFI scores were also calculated. Results AS patient groups and the control group had similar lipid profile parameters, thiol-disulfide homeostasis metrics, non-HDL cholesterol, oxLDL, and AIP levels (p>0.05). In contrast to AS patients receiving non-anti-TNF treatment, the BASDAI and BASFI scores, ESR, and CRP levels were lower in AS patients receiving anti-TNF treatment (p<0.05). In contrast to the controls, AS patients demonstrated high TOS and OSI values (p<0.05). Conclusions AS patients face increased oxidative stress that does not correlate with inflammatory status and disease activation level. Anti-TNF alpha treatment may reduce some risk factors of atherosclerotic CVD in AS patients.

Objectives Serum thyroglobulin (Tg) is a biochemical marker used in the follow-up of patients with differentiated thyroid cancer (DTC), but heterophile antibody interference may limit the clinical use of Tg. Case presentation In the first-year follow-up of a 31-year-old female patient who received treatment for papillary thyroid CA, a clinically significant difference was found in the Tg values measured by two different brand devices with similar measurement methods. Conclusions Despite the use of state-of-the-art laboratory equipment, heterophile antibodies in immunoassay analysis can still cause false high Tg measurements. Our case report shows that analytical errors can be avoided with attention and effective communication between clinicians and laboratory specialists. In this way, patients will be prevented from being misdiagnosed and/or exposed to treatment.

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