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A new strategy for on-site monitoring of cell cycle progression was proposed using cell chip technology. Cell synchronization has been utilized in intensive cellular research due to the fact that cells in different phases of the cell cycle exhibit different behaviors even when exposed to the same concentrations of drugs or toxicants. However, confirmation of cell cycle arrest in research is usually dependent on fluorescence-assisted cell sorting (FACS), which is laborious, time-consuming, and expensive. In this study, we employed a cell-chip-based electrochemical method to detect the cell-cycle-dependent electrochemical properties of cells. Electron transfer at the cell-electrode interface played a key role in our strategy and accurately reflected the redox activity of the cells in different phases. Rat pheochromocytoma cells were synchronized with thymidine and nocodazole, and well-defined current peaks from cells in the G1/S- and G2/M-phases were significantly different as determined by differential pulse voltammetry. FACS assay and Western blot analysis were used to validate the electrochemical findings. Hence, our cell-chip-based electrochemical method can be a useful tool in determining cell cycle progression easily and economically.

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