Electrochemical cell-based chip for the detection of toxic effects of bisphenol-A on neuroblastoma cells

Kafi, Md. Abdul; Kim, Tae-Hyung; An, Jeung Hee; Choi, Jeong‐Woo

doi:10.1016/j.bios.2010.12.030

Cited by 38 publications

(28 citation statements)

References 24 publications

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“…Recently, cell-based electrochemical method that is simple, sensitive, rapid, label-free, and non-toxic has gained attention for toxicity assessment192021. An in-situ electrochemical method was successfully developed and applied in drug screening and toxicity evaluation of heavy metals in our previous study222324, which was based on the electrical signals of the intermediates of cellular purine metabolism, and objectively revealed the cell viability at the molecular level25.…”

mentioning

confidence: 99%

Quantitative determination and toxicity evaluation of 2,4-dichlorophenol using poly(eosin Y)/hydroxylated multi-walled carbon nanotubes modified electrode

Zhu

Zhang

Wang

et al. 2016

Sci Rep

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This study aimed at developing simple, sensitive and rapid electrochemical approach to quantitatively determine and assess the toxicity of 2,4-dichlorophenol (2,4-DCP), a priority pollutant and has potential risk to public health through a novel poly(eosin Y, EY)/hydroxylated multi-walled carbon nanotubes composite modified electrode (PEY/MWNTs-OH/GCE). The distinct feature of this easy-fabricated electrode was the synergistic coupling effect between EY and MWNTs-OH that enabled a high electrocatalytic activity to 2,4-DCP. Under optimum conditions, the oxidation peak current enhanced linearly with concentration increasing from 0.005 to 0.1 μM and 0.2 to 40.0 μM, and revealed the detection limit of 1.5 nM. Moreover, the PEY/MWNTs-OH/GCE exhibited excellent electrocatalytic activity toward intracellular electroactive species. Two sensitive electrochemical signals ascribed to guanine/xanthine and adenine/hypoxanthine in human hepatoma (HepG2) cells were detected simultaneously. The sensor was successfully applied to evaluate the toxicity of 2,4-DCP to HepG2 cells. The IC50 values based on the two electrochemical signals are 201.07 and 252.83 μM, respectively. This study established a sensitive platform for the comprehensive evaluation of 2,4-DCP and posed a great potential to simplify environmental toxicity monitoring.

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mentioning

confidence: 99%

Quantitative determination and toxicity evaluation of 2,4-dichlorophenol using poly(eosin Y)/hydroxylated multi-walled carbon nanotubes modified electrode

Zhu

Zhang

Wang

et al. 2016

Sci Rep

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“…The three-electrode system composed of a cell-attached microgap electrode, a platinum (Pt) auxiliary electrode, and an Ag/AgCl/1M KCl reference electrode were used as previously described [35]. For electrochemical measurement, cells were washed with PBS (0.01M, pH 7.4).…”

Section: Methodsmentioning

confidence: 99%

3D graphene oxide-encapsulated gold nanoparticles to detect neural stem cell differentiation

2013

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Monitoring of stem cell differentiation and pluripotency is an important step for the practical use of stem cells in the field of regenerative medicine. Hence, a new non-destructive detection tool capable of in situ monitoring of stem cell differentiation is highly needed. In this study, we report a 3D graphene oxide-encapsulated gold nanoparticle that is very effective for the detection of the differentiation potential of neural stem cells (NSCs) based on surface-enhanced Raman spectroscopy (SERS). A new material, 3D GO-encapsulated gold nanoparticle, is developed to induce the double enhancement effect of graphene oxide and gold nanoparticle on SERS signals which is only effective for undifferentiated NSCs. The Raman peaks achieved from undifferentiated NSCs on the graphene oxide (GO)-encapsulated gold nanoparticles were 3.5 times higher than peaks obtained from normal metal structures and were clearly distinguishable from those of differentiated cells. The number of C=C bonds and the raman instensity at 1656cm−1 was found to show a positive correlation, which matches the differentiation state of the NSCs. Moreover, the substrate composed of 3D GO-encapsulated gold nanoparticles was also effective at distinguishing the differentiation state of single NSC by using electrochemical and electrical techniques. Hence, the proposed technique can be used as a powerful non-destructive in situ monitoring tool for the identification of the differentiation potential of various kinds of stem cells (mesenchymal, hematopoietic, and neural stem cells).

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“…7). It is well known that living cells have distinct cell line and cell cycle stage specific redox property [15,36]. Here, the peak is derived from the heathy cell that remained attached with electrode since dead cells are not able to bridge the gap [37].…”

Section: Electrical Read-out To Monitor Cell Proliferationmentioning

confidence: 99%

Chitosan-Graphene Oxide-Based Ultra-Thin and Flexible Sensor for Diabetic Wound Monitoring

et al. 2020

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This work presents a Chitosan-Graphene Oxide (CS-GO) based array of ultra-thin biosensors with gold (Au) based microgap (60µm) electrode. The cross-linked GO is shown to improve the stability of chitosan substrate in aqueous medium and compatibility with microfabrication steps. The sensor patch has been evaluated for label free monitoring by immobilizing the CS-GO surface with human dermal fibroblast (HDF) cells. The cyclic voltammetry (CV) of HDF cell immobilized CS-GO surface show quasi-reversible nature with characteristic cathodic peak at +300mV and anodic peak at-300mV. Both peaks are stable and repeatable up to 50-scan cycle without any potential shift. The device shows steady state peak enhancement (1.923-11.195nA) during the DHF cell growth period (0-96h). The redox peak enhancement correlates with the cell proliferation rates over time, indicating that it could be employed for investigation of cyto-physiological state against any endo and exogenous stimulation. In addition, the developed sensor-patch was used to detect a wide range of glucose from 1μM to 20mM in vitro with a sensitivity of 0.17µA/mM. Considering these, the presented sensor-patch has a great potential for the detection of glucose level, cell-health proliferation rate at the wound site and diabetic wound monitoring applications.

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Electrochemical cell-based chip for the detection of toxic effects of bisphenol-A on neuroblastoma cells

Cited by 38 publications

References 24 publications

Quantitative determination and toxicity evaluation of 2,4-dichlorophenol using poly(eosin Y)/hydroxylated multi-walled carbon nanotubes modified electrode

Quantitative determination and toxicity evaluation of 2,4-dichlorophenol using poly(eosin Y)/hydroxylated multi-walled carbon nanotubes modified electrode

3D graphene oxide-encapsulated gold nanoparticles to detect neural stem cell differentiation

Chitosan-Graphene Oxide-Based Ultra-Thin and Flexible Sensor for Diabetic Wound Monitoring

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