Effects of nanopatterned RGD peptide layer on electrochemical detection of neural cell chip

Kafi, Md. Abdul; Kim, Tae-Hyung; Yea, Cheol-Heon; Kim, Hyuncheol; Choi, Jeong‐Woo

doi:10.1016/j.bios.2010.07.049

Cited by 36 publications

(68 citation statements)

References 32 publications

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“…Phosphate buffered saline (PBS) (pH 7.4, 10 mM) solution consisting of 136.7 mM NaCl, 2.7 mM KCl, 9.7 mM Na 2 HPO 4 and 1.5 mM KH 2 PO 4 was purchased from Sigma-Aldrich. (((Arg-Gly-Asp) 3 2 -Lys) 2 -Ala-Cys (RGD-MAP-C) peptide was previously designed by our group 23 and synthesized by Peptron (Deajeon, Korea). F3 cells were provided from Dr. Kim (Chung-Ang University, Korea).…”

Section: Methodsmentioning

confidence: 99%

“…25 26 Moreover, we recently found that RGD peptide nanopatterned surface is more effective than RGD monolayer surface for increasing the cell binding affinity between the cell and electrode interface, which is most important for the sensitive detection of cell viability based on electrochemical tools. 23 To confirm this effectiveness of RGD peptide nanopatterned surface for human neural stem cells, the redox signals obtained from F3 cells during cyclic voltammetric measurements in the potential range from −0.3 V to +0.6 V was analyzed as shown in Figure 3. The difference between the potential peaks (E pc − E pa exceeded 0.29 V and the peak current ratio I pa /I pc = 1, which are indicators for the quasi-reversible character of the cell electrode process.…”

Section: Cyclic Voltammetry Of F3 Cells Attached On Peptide Nanopattementioning

confidence: 99%

“…These results are consistent with our previous study that proved the superiority of peptide nanopatterned by electrochemical method and cell adhesion strength assay. 23 tions of F3 cells and the current intensities of reduction peak, F3 cells were seeded on RGD-MAP-C peptide coated surface with different cell concentrations. The peak current was found to be increased with increasing cell concentration (Fig.…”

Section: Cyclic Voltammetry Of F3 Cells Attached On Peptide Nanopattementioning

confidence: 99%

“…20 Moreover, we fabricated arginine-glycine-aspartate (RGD) peptide 21 22 nanopatterned surface by self-assembly technique through nanoporous anodic alumina oxide (AAO) mask to improve the stem cell adhesion on electrode surface that directly effects on the sensitivity of electrochemical measurements. 23 After the confirmation of peptide nanopatterned surface and enhanced electrochemical signals from neural stem cells, the correlation between the stem cell viability and concentrations of malathion and -cypermethrin was intensively studied by both cyclic voltammetry and MTT viability assay.…”

Section: Introductionmentioning

confidence: 99%

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Fabrication of Stem Cell Chip with Peptide Nanopatterned Layer to Detect Cytotoxicity of Environmental Toxicants

Cho¹,

Kim²,

Kim³

et al. 2012

J. Nanosci. Nanotech.

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A stem cell chip with peptide nanopatterned layer was fabricated to detect the effects of environmental toxins on human neural stem cells (HB1 x F3) electrochemically. The cell chip was recently developed as in vitro monitoring tool for determining the cell viability simply and rapidly compared to the conventional methods. However, cell chip composed of neural stem cells have not been reported due to the difficulties for maintaining its stemness and cell attachment on the artificial electrode surface, which is critical for sensitive detection of cell viability electrochemically. In this study, we fabricated peptide nanopatterned layer on gold electrode for increasing the affinity between the stem cell and an artificial electrode surface by self-assembly technique. After the confirmation of fabricated nanopatterned surface, neural stem cells were immobilized on chip surface and the viability was measured by electrochemical method. Thereafter, neural stem cells were treated with two kinds of common environmental toxins, and the intensities of reduction peak obtained by cyclic voltammetry (CV) were decreased with the increase of concentrations of environmental toxins. These electrochemical results were validated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Our newly developed stem cell chip can be used as useful label-free analysis tool for detecting drug effects or for assessing the toxicity electrochemically.

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Section: Methodsmentioning

confidence: 99%

Section: Cyclic Voltammetry Of F3 Cells Attached On Peptide Nanopattementioning

confidence: 99%

Section: Cyclic Voltammetry Of F3 Cells Attached On Peptide Nanopattementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fabrication of Stem Cell Chip with Peptide Nanopatterned Layer to Detect Cytotoxicity of Environmental Toxicants

Cho¹,

Kim²,

Kim³

et al. 2012

J. Nanosci. Nanotech.

Self Cite

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“…17 Au surfaces have been used as a substrate for the formation of self-assembled monolayers of thiocompounds, such as cysteine (Cys) proteins. 18 In particular, the cell adhesion motif, RGD, and its derivatives, which are found in a variety of cellular adhesive molecules, have been widely employed as substrates to increase cellular attachment, adhesion, and proliferation.…”

Section: Differentiated Cells On the Two-dimensional Aumentioning

confidence: 99%

Electrochemical Detection of Human Mesenchymal Stem Cell Differentiation on Fabricated Gold Nano-Dot Cell Chips

An¹,

Kim²,

Park³

et al. 2015

j nanosci nanotechnol

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Human mesenchymal stem cells (MSCs) have the capacity for self-renewal and maintain pluripotency, which is defined by their ability to differentiate into cells such as osteoblasts, neurons, and glial cells. In this study, we report a method for defining the status of human MSCs based on electrochemical detection systems. Gold nano-dot structures were fabricated using a nanoporous alumina mask, and the structural formations were confirmed by scanning electron microscopy (SEM). Human MSCs were allowed to attach to RGD (Arg-Gly-Asp) peptide nanopatterned surfaces, and electrochemical tools were applied to the MSCs attached on the chip surface. The cultured MSCs were shown to differentiate into neural cell types, as indicated by immunocytochemical staining for tyrosine hydroxylase and beta tubulin III. Following treatment with basic fibroblast growth factor (bFGF) for 14 days, most of the B10 cells exhibited bipolar or multipolar morphology with branched processes, and the proportion of B10 cells expressing neuronal cell markers considerably increased. Electrophysiological recordings from MSCs treated with bFGF for 5-14 days were examined with cyclic voltammetry, and the electrochemical signals were shown to increase during differentiation from MSCs to neuronal cells. This human MSC cell line is a useful tool for studying organogenesis, specifically neurogenesis, and in addition, the cell line provides a valuable source of cells for cell therapy. The electrochemical measurement system proposed here could be utilized in electrical cell chips for numerous applications, including cell differentiation, disease diagnosis, drug detection, and on-site monitoring.

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Nanopatterned Surfaces for Stem‐Cell Engineering

El‐Said

Kim

Lee

et al. 2015

Stem‐Cell Nanoengineering

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Effects of nanopatterned RGD peptide layer on electrochemical detection of neural cell chip

Cited by 36 publications

References 32 publications

Fabrication of Stem Cell Chip with Peptide Nanopatterned Layer to Detect Cytotoxicity of Environmental Toxicants

Fabrication of Stem Cell Chip with Peptide Nanopatterned Layer to Detect Cytotoxicity of Environmental Toxicants

Electrochemical Detection of Human Mesenchymal Stem Cell Differentiation on Fabricated Gold Nano-Dot Cell Chips

Nanopatterned Surfaces for Stem‐Cell Engineering

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